Publication Date:
2004-06-19
Description:
Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA)expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4765737/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4765737/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Shav-Tal, Yaron -- Darzacq, Xavier -- Shenoy, Shailesh M -- Fusco, Dahlene -- Janicki, Susan M -- Spector, David L -- Singer, Robert H -- DOE63056/PHS HHS/ -- EB2060/EB/NIBIB NIH HHS/ -- GM42694/GM/NIGMS NIH HHS/ -- R01 EB002060/EB/NIBIB NIH HHS/ -- R01 GM042694/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Jun 18;304(5678):1797-800.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departments of Anatomy and Structural Biology and Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15205532" target="_blank"〉PubMed〈/a〉
Keywords:
Active Transport, Cell Nucleus
;
Adenosine Triphosphate/metabolism
;
Capsid Proteins/genetics/metabolism
;
Cell Line, Tumor
;
Cell Nucleus/*metabolism
;
Chromatin/metabolism
;
Cytoplasm/metabolism
;
Diffusion
;
Energy Metabolism
;
Fluorescence Recovery After Photobleaching
;
Globins/genetics/metabolism
;
Green Fluorescent Proteins
;
Humans
;
In Situ Hybridization, Fluorescence
;
Luminescent Proteins/genetics/metabolism
;
Peroxisomes/metabolism
;
Protein Biosynthesis
;
Proteins/genetics/*metabolism
;
RNA, Messenger/genetics/*metabolism
;
RNA-Binding Proteins/genetics/metabolism
;
Recombinant Fusion Proteins/metabolism
;
Transcription, Genetic
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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