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  • 1
    Publication Date: 2015-07-16
    Description: Parallel changes in body shape may evolve in response to similar environmental conditions, but whether such parallel phenotypic changes share a common genetic basis is still debated. The goal of this study was to assess whether parallel phenotypic changes could be explained by genetic parallelism, multiple genetic routes, or both. We first provide evidence for parallelism in fish shape by using geometric morphometrics among 300 fish representing five species pairs of Lake Whitefish. Using a genetic map comprising 3438 restriction site–associated DNA sequencing single-nucleotide polymorphisms, we then identified quantitative trait loci underlying body shape traits in a backcross family reared in the laboratory. A total of 138 body shape quantitative trait loci were identified in this cross, thus revealing a highly polygenic architecture of body shape in Lake Whitefish. Third, we tested for evidence of genetic parallelism among independent wild populations using both a single-locus method (outlier analysis) and a polygenic approach (analysis of covariation among markers). The single-locus approach provided limited evidence for genetic parallelism. However, the polygenic analysis revealed genetic parallelism for three of the five lakes, which differed from the two other lakes. These results provide evidence for both genetic parallelism and multiple genetic routes underlying parallel phenotypic evolution in fish shape among populations occupying similar ecological niches.
    Electronic ISSN: 2160-1836
    Topics: Biology
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  • 2
    Publication Date: 1998-05-23
    Description: Surveys of risk behaviors have been hobbled by their reliance on respondents to report accurately about engaging in behaviors that are highly sensitive and may be illegal. An audio computer-assisted self-interviewing (audio-CASI) technology for measuring those behaviors was tested with 1690 respondents in the 1995 National Survey of Adolescent Males. The respondents were randomly assigned to answer questions using either audio-CASI or a more traditional self-administered questionnaire. Estimates of the prevalence of male-male sex, injection drug use, and sexual contact with intravenous drug users were higher by factors of 3 or more when audio-CASI was used. Increased reporting was also found for several other risk behaviors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Turner, C F -- Ku, L -- Rogers, S M -- Lindberg, L D -- Pleck, J H -- Sonenstein, F L -- R01-HD30861/HD/NICHD NIH HHS/ -- New York, N.Y. -- Science. 1998 May 8;280(5365):867-73.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Program in Health and Behavior Measurement at the Research Triangle Institute, 1635 M Street, NW, Washington, DC 20036, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9572724" target="_blank"〉PubMed〈/a〉
    Keywords: Adolescent ; Adolescent Behavior ; *Computers ; Data Collection/*methods ; Ethnic Groups ; Humans ; Interviews as Topic/*methods ; Male ; Privacy ; Risk-Taking ; *Sexual Behavior ; Socioeconomic Factors ; *Substance-Related Disorders ; Surveys and Questionnaires ; United States ; *Violence
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 2008-08-30
    Description: Experimental estimates of the effects of selection on genes determining adaptive traits add to our understanding of the mechanisms of evolution. We measured selection on genotypes of the Ectodysplasin locus, which underlie differences in lateral plates in threespine stickleback fish. A derived allele (low) causing reduced plate number has been fixed repeatedly after marine stickleback colonized freshwater from the sea, where the ancestral allele (complete) predominates. We transplanted marine sticklebacks carrying both alleles to freshwater ponds and tracked genotype frequencies over a generation. The low allele increased in frequency once lateral plates developed, most likely via a growth advantage. Opposing selection at the larval stage and changing dominance for fitness throughout life suggest either that the gene affects additional traits undergoing selection or that linked loci also are affecting fitness.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Barrett, Rowan D H -- Rogers, Sean M -- Schluter, Dolph -- New York, N.Y. -- Science. 2008 Oct 10;322(5899):255-7. doi: 10.1126/science.1159978. Epub 2008 Aug 28.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Zoology Department and Biodiversity Research Centre, University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada. rbarrett@zoology.ubc.ca〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18755942" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Biological Evolution ; Body Size ; Bone Development/*genetics ; Bone and Bones/anatomy & histology ; Ecosystem ; Ectodysplasins/*genetics ; Fresh Water ; Gene Frequency ; Genotype ; Microsatellite Repeats ; Oceans and Seas ; Reproduction ; Seawater ; *Selection, Genetic ; Sexual Maturation ; Smegmamorpha/*anatomy & histology/*genetics/growth & development
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Publication Date: 2009-01-31
    Description: Desert locusts, Schistocerca gregaria, show extreme phenotypic plasticity, transforming between a little-seen solitarious phase and the notorious swarming gregarious phase depending on population density. An essential tipping point in the process of swarm formation is the initial switch from strong mutual aversion in solitarious locusts to coherent group formation and greater activity in gregarious locusts. We show here that serotonin, an evolutionarily conserved mediator of neuronal plasticity, is responsible for this behavioral transformation, being both necessary if behavioral gregarization is to occur and sufficient to induce it. Our data demonstrate a neurochemical mechanism linking interactions between individuals to large-scale changes in population structure and the onset of mass migration.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Anstey, Michael L -- Rogers, Stephen M -- Ott, Swidbert R -- Burrows, Malcolm -- Simpson, Stephen J -- Biotechnology and Biological Sciences Research Council/United Kingdom -- New York, N.Y. -- Science. 2009 Jan 30;323(5914):627-30. doi: 10.1126/science.1165939.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19179529" target="_blank"〉PubMed〈/a〉
    Keywords: 5-Hydroxytryptophan/metabolism/pharmacology ; *Animal Migration ; Animals ; *Behavior, Animal/drug effects ; Crowding ; *Flight, Animal ; Ganglia, Invertebrate/*metabolism ; Grasshoppers/drug effects/*physiology ; Logistic Models ; Neural Pathways/physiology ; Odors ; Pest Control ; Photic Stimulation ; Physical Stimulation ; Population Density ; Serotonin/biosynthesis/*metabolism/pharmacology ; Serotonin Antagonists/pharmacology ; Serotonin Receptor Agonists/pharmacology ; Social Behavior
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 17 (1998), S. 656-660 
    ISSN: 1432-203X
    Keywords: Key wordsJuncus accuminatus ; Callus regeneration ; Medium pH ; Phytoremediation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A reliable callus regeneration and shoot multiplication system for wetland monocot Juncus accuminatus has been established. Callus was induced from 6-day-old seedlings on Murashige and Skoog medium supplemented with 5 mg/l picloram. The callus differentiated into shoots upon transfer to 5 mg/l benzyladenine (BA)-supplemented medium. Effects of medium pH (3.8–7.8) and source of callus (grown in the dark or continuous light) on regeneration were determined. Both parameters significantly influenced regeneration. Regenerated shoots were multiplied by subculturing shoots onto 5 mg/l BA medium at 4-week intervals. The regenerated shoots were rooted on 0.1 mg/l naphthaleneacetic-acid-supplemented medium. The rooted plants were transferred to pots containing a commercial potting mix and established in the greenhouse. Plants covered with plastic grew faster and flowered earlier than uncovered plants. All plants set viable seeds.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Photosynthesis research 21 (1989), S. 93-106 
    ISSN: 1573-5079
    Keywords: higher plant photosynthesis ; chlorophyll a fluorescence ; plastoquinone pool ; photoautotrophic soybean cell ; diuron ; atrazine ; primary (quinone) electron acceptor ; QB/QB -, (soybean, spinach)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report here the first measurements on chlorophyll (Chl) a fluorescence characteristics of photoautotrophic soybean cells (cell lines SB-P and SBI-P). The cell fluorescence is free from severe distortion problems encountered in higher plant leaves. Chl a fluorescence spectra at 77 K show, after correction for the spectral sensitivity of the photomultiplier and the emission monochromator, peaks at 688, 696 and 745 nm, representing antenna systems of photosystem II-CP43 and CP47, and photosystem I, respectively. Calculations, based on the complementary area over the Chl a fluorescence induction curve, indicated a ratio of 6 of the mobile plastoquinone (including QB) to the primary stable electron acceptor, the bound plastoquinone QA. A ratio of one between the secondary stable electron acceptor, bound plastoquinone QB, and its reduced form QB - was obtained by using a double flash technique. Owing to this ratio, the flash number dependence of the Chl a fluorescence showed a distinct period of four, implying a close relationship to the ‘S’ state of the oxygen evolution mechanism. Analysis of the QA - reoxidation kinetics showed (1) the halftime of each of the major decay components (∼ 300 μs fast and ∼ 30 ms slow) increases with the increase of diuron and atrazine concentrations; and (2) the amplitudes of the fast and the slow components change in a complementary fashion, the fast component disappearing at high concentrations of the inhibitors. This implies that the inhibitors used are able to totally displace QB. In intact soybean cells, the relative amplitude of the 30 ms to 300 μs component is higher (40:60) than that in spinach chloroplasts (30:70), implying a larger contribution of the centers with unbound QB. SB-P and SBI-P soybean cells display a slightly different sensitivity of QA - decay to inhibitors.
    Type of Medium: Electronic Resource
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  • 7
  • 8
    Publication Date: 2011-12-08
    Description: Greenstreet, S. P. R., Fraser, H. M., Rogers, S. I., Trenkel, V. M., Simpson, S. D., and Pinnegar, J. K. 2012. Redundancy in metrics describing the composition, structure, and functioning of the North Sea demersal fish community. – ICES Journal of Marine Science, 69: 8–22. Broader ecosystem management objectives for North Sea demersal fish currently focus on restoring community size structure. However, most policy drivers explicitly concentrate on restoring and conserving biodiversity, and it has not yet been established that simply restoring demersal fish size composition will be sufficient to reverse declines in biodiversity and ensure a generally healthy community. If different aspects of community composition, structure, and function vary independently, then to monitor all aspects of community general health will require application of a suite of metrics. This assumes low redundancy among the metrics used in any such suite and implies that addressing biodiversity issues specifically will require explicit management objectives for particular biodiversity metrics. This issue of metric redundancy is addressed, and 15 metrics covering five main attributes of community composition, structure, and function are applied to groundfish survey data. Factor analysis suggested a new interpretation of the metric information and indicated that a minimum suite of seven metrics was necessary to ensure that all changes in the general health of the North Sea demersal fish community were monitored properly. Covariance among size-based and species-diversity metrics was low, implying that restoration of community size structure would not necessarily reverse declines in species diversity.
    Print ISSN: 1054-3139
    Electronic ISSN: 1095-9289
    Topics: Biology , Geosciences , Physics
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  • 9
    Publication Date: 2015-10-09
    Description: Black carbon (BC) aerosol emitted by boreal fires has the potential to accelerate losses of snow and ice in many areas of the Arctic, yet the importance of this source relative to fossil fuel BC emissions from lower latitudes remains uncertain. Here we present measurements of the isotopic composition of BC and organic carbon (OC) aerosols collected at two locations in interior Alaska during the summer of 2013, as part of NASA's Carbon in Arctic Reservoirs Vulnerability Experiment. We isolated BC from fine air particulate matter (PM 2.5 ) and measured its radiocarbon (Δ 14 C) content with accelerator mass spectrometry (AMS). We show that fires were the dominant contributor to variability in carbonaceous aerosol mass in interior Alaska during the summer by comparing our measurements with satellite data, measurements from an aerosol network, and predicted concentrations from a fire inventory coupled to an atmospheric transport model. The Δ 14 C of BC from boreal fires was 131 ± 52‰ in year 2013 when the Δ 14 C of atmospheric CO 2 was 23 ± 3‰, corresponding to a mean fuel age of 20 years. Fire-emitted OC had a similar Δ 14 C (99 ± 21‰) as BC, but during background (low fire) periods OC (45 to 51‰) was more positive than BC (−354 to −57‰). We also analyzed the carbon and nitrogen elemental and stable isotopic composition of the PM 2.5 . Fire-emitted aerosol had an elevated carbon to nitrogen (C/N) ratio (29 ± 2) and δ 15 N (16 ± 4‰). Aerosol Δ 14 C and δ 13 C measurements were consistent with a mean depth of burning in organic soil horizons of 20 cm (and a range of 8 to 47 cm). Our measurements of fire-emitted BC and PM 2.5 composition constrain the endmember of boreal forest fire contributions to aerosol deposition in the Arctic and may ultimately reduce uncertainties related to the impact of a changing boreal fire regime on the climate system.
    Print ISSN: 0886-6236
    Electronic ISSN: 1944-9224
    Topics: Biology , Chemistry and Pharmacology , Geography , Geosciences , Physics
    Published by Wiley on behalf of American Geophysical Union (AGU).
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  • 10
    Publication Date: 2011-03-16
    Description: Elastin enables the reversible deformation of elastic tissues and can withstand decades of repetitive forces. Tropoelastin is the soluble precursor to elastin, the main elastic protein found in mammals. Little is known of the shape and mechanism of assembly of tropoelastin as its unique composition and propensity to self-associate has hampered structural studies. In this study, we solve the nanostructure of full-length and corresponding overlapping fragments of tropoelastin using small angle X-ray and neutron scattering, allowing us to identify discrete regions of the molecule. Tropoelastin is an asymmetric coil, with a protruding foot that encompasses the C-terminal cell interaction motif. We show that individual tropoelastin molecules are highly extensible yet elastic without hysteresis to perform as highly efficient molecular nanosprings. Our findings shed light on how biology uses this single protein to build durable elastic structures that allow for cell attachment to an appended foot. We present a unique model for head-to-tail assembly which allows for the propagation of the molecule’s asymmetric coil through a stacked spring design.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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