Publication Date:
2002-09-28
Description:
Insecticide resistance is one of the most widespread genetic changes caused by human activity, but we still understand little about the origins and spread of resistant alleles in global populations of insects. Here, via microarray analysis of all P450s in Drosophila melanogaster, we show that DDT-R, a gene conferring resistance to DDT, is associated with overtranscription of a single cytochrome P450 gene, Cyp6g1. Transgenic analysis of Cyp6g1 shows that overtranscription of this gene alone is both necessary and sufficient for resistance. Resistance and up-regulation in Drosophila populations are associated with a single Cyp6g1 allele that has spread globally. This allele is characterized by the insertion of an Accord transposable element into the 5' end of the Cyp6g1 gene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Daborn, P J -- Yen, J L -- Bogwitz, M R -- Le Goff, G -- Feil, E -- Jeffers, S -- Tijet, N -- Perry, T -- Heckel, D -- Batterham, P -- Feyereisen, R -- Wilson, T G -- ffrench-Constant, R H -- New York, N.Y. -- Science. 2002 Sep 27;297(5590):2253-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12351787" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Animals
;
Animals, Genetically Modified
;
Base Sequence
;
Chromosome Mapping
;
Cytochrome P-450 Enzyme System/*genetics/metabolism
;
*Ddt
;
Drosophila Proteins/*genetics/metabolism
;
Drosophila melanogaster/enzymology/*genetics
;
Gene Expression Profiling
;
Gene Expression Regulation, Enzymologic
;
*Genes, Insect
;
Insecticide Resistance/*genetics
;
*Insecticides/metabolism
;
Introns
;
Molecular Sequence Data
;
Oligonucleotide Array Sequence Analysis
;
Phylogeny
;
Reverse Transcriptase Polymerase Chain Reaction
;
Sequence Analysis, DNA
;
Substrate Specificity
;
Transcription, Genetic
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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