Publication Date:
2008-01-26
Description:
The chromosome passenger complex (CPC) controls chromosome congression, kinetochore-microtubule attachments, and spindle checkpoint signaling during mitosis. Aurora-B kinase is the catalytic subunit of the CPC. To understand how a single kinase can regulate such diverse events, we have investigated the activation of Aurora-B and describe two distinct activation mechanisms. First, Aurora-B activation in vitro requires two cofactors, telophase disc-60kD (TD-60) and microtubules. TD-60 is critical to localize both the CPC and Haspin kinase activity to centromeres and thus regulates Aurora-B at several levels. Second, Aurora-B substrates can inhibit kinase activation, and this is relieved by phosphorylation of these substrates by the centromeric kinases Plk1 and Haspin. These regulatory mechanisms suggest models for phosphorylation by Aurora-B of centromeric substrates at unaligned chromosomes and merotelic attachments.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Rosasco-Nitcher, Sara E -- Lan, Weijie -- Khorasanizadeh, Sepideh -- Stukenberg, P Todd -- R01GM063045-06/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2008 Jan 25;319(5862):469-72. doi: 10.1126/science.1148980.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Molecular Genetics, University of Virginia Medical School, Charlottesville, VA 22908, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18218899" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Aurora Kinases
;
Cell Cycle Proteins/metabolism
;
Centromere/*metabolism
;
Chromosomal Proteins, Non-Histone/*metabolism
;
Enzyme Activation
;
Histones/metabolism
;
Intracellular Signaling Peptides and Proteins/metabolism
;
Kinesin/metabolism
;
Microtubules/*metabolism
;
*Mitosis
;
Phosphorylation
;
Protein-Serine-Threonine Kinases/*metabolism
;
Xenopus/metabolism
;
Xenopus Proteins/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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