Publication Date:
1993-03-05
Description:
The actions of many hormones and neurotransmitters are mediated by the members of a superfamily of receptors coupled to heterotrimeric guanine nucleotide-binding proteins (G proteins). These receptors are characterized by a highly conserved topographical arrangement in which seven transmembrane domains are connected by intracellular and extracellular loops. The interaction between these receptors and G proteins is mediated in large part by the third intracellular loop of the receptor. Coexpression of the third intracellular loop of the alpha 1B-adrenergic receptor with its parent receptor inhibited receptor-mediated activation of phospholipase C. The inhibition extended to the closely related alpha 1C-adrenergic receptor subtype, but not the phospholipase C-coupled M1 muscarinic acetylcholine receptor nor the adenylate cyclase-coupled D1A dopamine receptor. These results suggest that the receptor-G protein interface may represent a target for receptor antagonist drugs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Luttrell, L M -- Ostrowski, J -- Cotecchia, S -- Kendall, H -- Lefkowitz, R J -- HL16037/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1993 Mar 5;259(5100):1453-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medicine, Duke University Medical Center, Durham, NC 27710.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8383880" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Base Sequence
;
Cell Line
;
Cloning, Molecular
;
Cyclic AMP/metabolism
;
Cytoplasm/metabolism
;
GTP-Binding Proteins/*metabolism
;
Globins/genetics
;
Glutathione Transferase/genetics/metabolism
;
Humans
;
Inositol Phosphates/metabolism
;
Kinetics
;
Molecular Sequence Data
;
Muscarinic Antagonists
;
Oligodeoxyribonucleotides
;
Plasmids
;
Protein Structure, Secondary
;
Receptors, Adrenergic, alpha/genetics/*metabolism
;
Receptors, Dopamine D1/antagonists & inhibitors/genetics/*metabolism
;
Receptors, Muscarinic/genetics/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
*Signal Transduction
;
Transfection
;
Type C Phospholipases/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink