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  • 1
    Electronic Resource
    Electronic Resource
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 110 (2000), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Senescence of lower leaves (LS) begins before anthesis in sunflower crop canopies. Using isolated field-grown sunflower plants, it has previously been shown that pre-anthesis LS is dependent on photosynthetic photon flux density (PPFD) and is hastened by increases in far-red light. We tested the hypothesis that increasing the red/far-red ratio (R/FR) perceived by basal leaves within canopies delays LS. To do this, light impinging on the lower surface of north-oriented 8th leaves (cotyledons=0) of crops with maximum leaf area indexes of 3.3 (Experiment 1) and 2.4 (Experiment 2) was enriched (+8.33 μmol m−2 s−1) with red light using light emitting diode (LED) panels. LED panels constructed with unlit LED or with green LED (PPFD slightly greater than the red LED panels, to compensate for lower efficiency) were used as controls. Compared with controls, additional R significantly (P〈0.05) increased R/FR perceived by the lower surface and significantly (P〈0.01) delayed LS. On average, leaf duration, as time between full expansion and a 70% diminution of chlorophyll content, was 5 days greater for leaves receiving extra red light (maximum observed LD=27 days). We conclude that an increase in the R/FR ratio can delay LS in crop canopies.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 20 (1997), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Ectopic overexpression of an oat PHYA cDNA in tobacco under the cauliflower mosaic virus 35S promoter results in plants with reduced morphological responses to far-red radiation (FR). We have tested the hypothesis that it is possible to molecularly ‘mask’ steins and leaves to FR-induced elongation and senescence responses by targeting the over-expression of PHYA with appropriate promoters. Oat PHYA was expressed in tobacco (Nicotiana tabacum L. cv Xanthi) under the 35S and two Arabidopsis promoters: UBQ1 and CAB. The internodes of wild type, UBQ:PHYA, and CAB:PHYA plants, which exhibited little or no oat PHYA overexpression, responded to localized FR treatments with a marked increase in elongation. In contrast, 35S:PHYA plants, which overexpressed PHYA to high levels in all parts of the shoot, did not respond to FR treatments directed to their stems. Leaf senescence responses to FR were remarkably localized, and sensitivity to FR was also inversely correlated with the local PHYA expression level. Thus, chlorophyll content, specific leaf weight, and nitrate reductase activity in leaf spots treated with FR were highly reduced in wild type and UBQ:PHYA plants, but not in the CAB:PHYA and 35S:PHYA counterparts. Our results suggest that it may be feasible to obtain transgenic crop plants in which certain organs or tissues are made ‘blind’ to phytochrome-perceived signals of canopy density, but whose general photomorphogenic competence is not greatly disturbed.
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 96 (1996), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Basal leaves frequently senesce before anthesis in high population density crops. This paper evaluates the hypothesis that quantitative and qualitative changes in the light environment associated with a high leaf area index (LAI) trigger leaf senescence in sunflower (Helianthus annuus L.) canopies. Mean leaf duration (LD, time from achievement of maximum leaf area) of leaf 8 was significantly (P 〈 0.05) reduced from 51 to 19 days as crop population density was increased from 0.47 to 4.76 plants m−2. High compared to low plant population density was associated with earlier reduction in the photosynthetically active radiation (PAR) and red/far-red ratio (R/FR) reaching the target leaf. However the changes in R/FR preceded those in PAR. When the light environment of individual leaves of isolated plants growing under field conditions was manipulated using filters and FR-reflecting mirrors, LD was positively and linearly related with the mean daily PAR (MDR) received in the FR- (no FR enrichment) treatments. FR enrichment of light reaching the abaxial surface of the leaf significantly (P 〈 0.05) reduced LD by 9 days at intermediate PAR levels with respect to FR-controls, but did not affect LD at the maximum PAR used in these experiments. However, when light reaching both leaf surfaces was enriched with FR, LD (for leaves receiving maximum PAR) was 13 days shorter than that of the FR- control. These results show that basal leaf senescence in sunflower is enhanced both by a decrease in PAR and by a decrease in R/FR.
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  • 4
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: A study was made of the effects of solar ultraviolet-B radiation (UV-B) on the growth of the dominant plant species of a shrub-dominated ecosystem in Tierra del Fuego. This part of southern Argentina can be under the direct influence of the Antarctic ‘ozone hole’ during the austral spring and lingering ozone-depleted air during the summer. The plant community is dominated by an evergreen shrub (Chiliotrichum diffusum) with an herbaceous layer of Gunnera magellanica and Blechnum penna-marina in the interspaces between the shrubs. Inspections of ozone trends indicate that the springtime and summertime ozone column over Tierra del Fuego has decreased by 10–13% from 1978/9 to 1998/9. In a set of well-replicated field plots, solar UV-B was reduced to approximately 15–20% of the ambient UV-B using plastic films. Polyester films were used to attenuate UV-B radiation and UV-transparent films (∼90% UV-B transmission) were used as control. Treatments were imposed during the growing season beginning in 1996 and continued for three complete growing seasons. Stem elongation of the shrub C. diffusum was not affected by UV-B attenuation in any of the three seasons studied. However, frond length of B. penna-marina under attenuated UV-B was significantly greater than that under near-ambient UV-B in all three seasons. Attenuation of solar UV-B also promoted the expansion of G. magellanica leaves in two of the growing seasons. Differences between treatments in leaf or frond length in B. penna-marina and G. magellanica did not exceed 12%. Another significant effect of UV-B attenuation was a promotion of insect herbivory in G. magellanica, with a 25–75% increase in the leaf area consumed. Changes in plant phenology or relative species cover were not detected within the time frame of this study. The results suggest that the increase in UV-B radiation associated with the erosion of the ozone layer might be affecting the functioning of this ecosystem to some degree, particularly by inhibiting the growth of some plant species and by altering plant–insect interactions.
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  • 5
    ISSN: 1365-2486
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Geography
    Notes: Stratospheric ozone depletion occurs over Tierra del Fuego, southern Argentina and Chile, in the austral spring and summer due to the precession of the Antarctic ‘ozone hole’ and the general erosion of the ozone layer. Plots receiving either near-ambient or reduced UV-B radiation were established using different louvered plastic film filters over Sphagnum bog and Carex fen ecosystems in October 1996. In the Sphagnum bog system, growth measurements during the late spring and summer showed no significant differences in the moss Sphagnum magellanicum, or the vascular plants (Empetrum rubrum, Nothofagus antarctica, and Tetroncium magellanicum) between near-ambient and attenuated UV-B radiation treatments. In the Carex fen system, leaf length and spike height did not differ in the two dominant species, Carex decidua and C. curta, between UV-B radiation treatments. The length of individual spikelets of C. curta under near-ambient UV-B radiation was less than under the reduced UV-B radiation treatment, but this was not evident in C. decidua. No differences in seed number, seed mass, or viability were seen in either Carex species between the UV-B treatments. Two important constituents of the microfauna that inhabit the Sphagnum bog are testate amoebae and rotifers. These both appeared to be more numerous under near-ambient UV-B radiation than under reduced UV-B radiation. The subtle responses of the Sphagnum and Carex ecosystems may become more apparent in subsequent years as the treatments are continued. Trophic-level changes, such as the differences in number of amoebae and rotifers, may be more sensitive to solar UV-B radiation than growth and productivity of the vegetation.
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  • 6
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 28 (1972), S. 1720-1724 
    ISSN: 1600-5740
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
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  • 7
    ISSN: 0378-1119
    Keywords: Recombinant DNA ; immunoglobulin heavy chain ; mouse ; polyadenylation ; transcription termination ; μ gene
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 178 (1991), S. 8-15 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-1939
    Keywords: Key wordsGunnera magellanica ; Ultraviolet ; Ozone depletion ; Herbivory ; Defense
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined the effects of solar ultraviolet-B radiation (UVB) on plant-herbivore interactions in native ecosystems of the Tierra del Fuego National Park (southern Argentina), an area of the globe that is frequently under the Antarctic “ozone hole” in early spring. We found that filtering out solar UVB from the sunlight received by naturally-occurring plants of Gunnera magellanica, a creeping perennial herb, significantly increased the number of leaf lesions caused by chewing insects. Field surveys suggested that early-season herbivory was principally due to the activity of moth larvae (Lepidoptera: Noctuidae). Manipulative field experiments showed that exposure to solar UVB changes the attractiveness of G. magellanica leaf tissue to natural grazers. In a laboratory experiment, locally caught moth caterpillars tended to eat more tissue from leaves grown without UVB than from leaves exposed to natural UVB during development; however, the difference between treatments was not significant. Leaves grown under solar UVB had slightly higher N levels than leaves not exposed to UVB; no differences between UVB treatments in specific leaf mass, relative water content, and total methanol-soluble phenolics were detected. Our results show that insect herbivory in a natural ecosystem is influenced by solar UVB, and that this influence could not be predicted from crude measurements of leaf physical and chemical characteristics and a common laboratory bioassay.
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