ISSN:
1617-4623
Keywords:
PEP carboxylase
;
Azotobacter chroococcum
;
Nitrogen fixation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Azotobacter chroococcum Fos 189 is a Tn1-induced mutant which, unlike the parent strain MCD1, does not fix nitrogen in air when provided with glucose or pyruvate as sole carbon sources. Fos 189 showed 5% of parental activity for phosphoenolpyruvate carboxylase though PEP synthetase activity was normal. The A. chroococcum phosphoenolpyruvate carboxylase (ppc) gene was isolated after complementation of an appropriate Escherichia coli mutant using a broad host range gene bank prepared from A. chroococcum genomic DNA. The gene was localised by transposon mutagenesis and subcloning on a minimum DNA fragment of 6.6 kb. Broad host range plasmids containing the A. chroococcum ppc gene complemented the mutation in Fos 189 thereby restoring aerotolerant nitrogen fixation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00328143
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