Publication Date:
2015-01-28
Description:
Msmeg_0515, a gene fromMycobacterium smegmatisstrain 155 encoding the ligand-binding domain, AgaE, of a putative ABC sugar transporter system, has been cloned into a pET-28a vector system, overexpressed inEscherichia coliand purified. The truncated protein lacking the first 27 residues, which correspond to a N-terminal signal sequence, was crystallized using the sitting-drop vapour-diffusion technique. The crystals of this protein diffracted to 1.48 Å resolution and belonged to space groupP212121, with unit-cell parametersa= 64.06,b= 69.26,c= 100.74 Å, α = β = γ = 90° and with one molecule in the asymmetric unit.
Electronic ISSN:
2053-230X
Topics:
Biology
,
Chemistry and Pharmacology
,
Geosciences
,
Physics
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