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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and characterization of nuclear ghosts from cultured HeLa cells (1975)
    s.l. : American Chemical Society
    Biochemistry 14 (1975), S. 3005-3013 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00684a033
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  • 2
    Electronic Resource
    Electronic Resource
    Deoxyribonuclease I generates single-stranded gaps in chromatin deoxyribonucleic acid (1980)
    s.l. : American Chemical Society
    Biochemistry 19 (1980), S. 2977-2992 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00554a024
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  • 3
    Electronic Resource
    Electronic Resource
    Trichomonas vaginalis: Dominant G2 Period and G2 Phase Arrest in a Representative of an Early Branching Eukaryotic Lineage (1994)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 41 (1994), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: . Eukaryotic mitotic cell cycles have been extensively studied in yeasts and vertebrate cells but little is known about cell cycle mechanisms in early branches of the eukaryotic lineage. Trichomonas vaginalis represents one of the earliest branching eukaryotic lineages available for study. In contrast with most yeasts and vertebrate cells, the T. vaginalis G2 period was prolonged, comprising 50 to 58% of the cell population. Hydroxyurea, aphidicolin, and excess thymidine, all of which arrest yeasts and vertebrate cells at the G1/S phase boundary, had no effect on the T. vaginalis cell cycle, probably due to the known absence of synthetic pathways. The antimicrotubule mitotic inhibitors, colchicine and nocodazole, induced G2 phase synchrony. Metronidazole, a therapeutic reagent, also caused G2 phase arrest. These observations suggest that T. vaginalis is similar to yeasts and vertebrate cells in G2 and M phases, but the parasite's G1/S phase transition is distinctive. The results also suggest potentially therapeutic, anti-trichomonad activity of microtubule inhibitors such as nocodazole. The cultured parasite may prove useful as a model for the mitotic cell cycle in the absence of G1/S phase transitional activities universal in yeasts and vertebrate cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1994.tb06098.x
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  • 4
    Electronic Resource
    Electronic Resource
    Trichomonas vaginalis and early evolving DNA and protein sequences of the CDC2/28 protein kinase family (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Molecular microbiology 8 (1993), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: The human sexually transmittted parasite Trichomonas vaginalis is a representative of one of the three earliest evolving eukaryotic lineages. We investigated whether T. vaginalis has DNA sequences and peptides related to cell division control molecules universal among yeasts and higher eukaryotes. A T. vaginalis ceil division control (CDC2/28) homologue was amplified by the polymerase chain reaction and sequenced. The absolute similarity with other CDC2/28 genes was 47%, with conservative replacement similarity of 67%. Western blots demonstrated a single T. vaginalis peptide reactive with antiserum to the PSTAIRE peptide, an expressed component of CDC2/28 genes in higher eukaryotes. Although eukaryotic, T. vaginalis has properties similar to those of bacteria and is the earlist evolving eukaryote reported to possess CDC2/28 DNA and peptide homologues. These observations suggest that the molecular origins of cell division control in eukaroytes preceded mitochondria, 28S ribosomes and regulated glycolysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2958.1993.tb01595.x
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular and phylogenetic analysis of PCR-amplified cyclin-dependent kinase (CDK) family sequences from representatives of the earliest available lineages of eukaryotes (1995)
    Springer
    Journal of molecular evolution 41 (1995), S. 407-413 
    Details
    ISSN: 1432-1432
    Keywords: Giardia ; Trichomonas ; CDK ; CDC ; Unicellular ; Metazoa ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyclin-dependent kinase (CDK) and cell division control (CDC2) sequences are strongly conserved among eukaryotes and may complement the use of other sequence families in eukaryotic phylogenetic inference. We synthesized degenerate PCR primers to amplify the catalytic region of CDK homologs in representatives of the earliest available lineages of eukaryotes. CDK family sequence-based, maximum-likelihood distance measurements with neighbor joining, and Fitch-Margoliash least-squares analyses produced unrooted dendrograms that included protists, yeasts, and higher eukaryotes. Bootstrap confidence estimates supported CDK-based phylogenetic groupings among the protists, fungi, and vertebrates although resolution within these groups was often insignificant. However, Trichomonas vaginalis and Giardia lamblia exhibited two of the most divergent CDK-like sequences consistent with rRNA-phylogenetic inference of early divergence of these eukaryotic lineages. In the evolution from unicellular to multicellular organisms, a constellation of amino acid residues aligning with the human, CDK N-terminal β sheet may have undergone abrupt replacement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00160311
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  • 6
    Electronic Resource
    Electronic Resource
    Analysis of inactive X chromosome structure by in situ nick translation (1985)
    Springer
    Chromosoma 92 (1985), S. 209-213 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Nick translation assays of fixed interphase female fibroblasts with tritiated nucleotides demonstrated a characteristic absence of label over sex chromatin. The chromatin bodies were nearly always peripheral in location and a ribbon of nick translatable DNA was detected between the sex chromatin and the nuclear envelope. High voltage electron microscopy indicated the possibility of a special nuclear envelope attachment region. The apparent resistance of sex chromatin to nick translation did not appear to be due to resistance to DNase I attack.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00348695
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  • 7
    Electronic Resource
    Electronic Resource
    Chromatin structure of active and inactive human X-linked phosphoglycerate kinase gene (1986)
    Springer
    Somatic cell and molecular genetics 12 (1986), S. 73-80 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have carried out a comparative analysis of DNase I sensitivity of the active and inactive X-linked phosphoglycerate kinase (Pgk)genes in human lymphoblast and fibroblast cultured cells. Three DNase I-sensitive regions were detected: a 5′ hypersensitive site, a sensitive region in the interior of the gene and a 3′ slightly sensitive site which we previously reported and have now mapped with some precision. A comparison of these sensitive sites in single and multiple X cell lines indicates that the sensitive sites are unique to the active X chromosome. A similar study of an X-linked Pgkpseudogene shows no difference in DNase I sensitivity between the pseudogenes on the active and inactive X chromosomes. These latter results imply that sex chromatin does not confer a unique level of DNase I resistance to DNA on the inactive X chromosome. The exact role of sex chromatin in differential DNase I sensitivity of genes on the inactive and active X chromosomes is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01560729
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  • 8
    Electronic Resource
    Electronic Resource
    Mapping of human autosomal phosphoglycerate kinase sequence to chromosome 19 (1986)
    Springer
    Somatic cell and molecular genetics 12 (1986), S. 395-401 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In order to map human PGKsequences, DNA was prepared from 55 human-mouse somatic cell lines. The DNA was digested to completion with HindIII and Southern filters prepared. These filters were hybridized at high stringency conditions to a human PGKcDNA. Mouse and human X-linked and autosomal bands were distinguished and, in addition to known X-linked sequences, two autosomal PGKsequences were mapped: a 1-kb band to chromosome 19 and a 5-kb band to chromosome 6. The PGKcDNA probe was also hybridized to flow-sorted chromosomes confirming the presence of PGK sequences on the X chromosome and chromosomes 6 and 19.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01570734
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  • 9
    Electronic Resource
    Electronic Resource
    A hemizygous short tandem repeat polymorphism 3′ to the human phosphoglycerate kinase gene (1999)
    Springer
    Molecular biology reports 26 (1999), S. 159-165 
    Details
    ISSN: 1573-4978
    Keywords: PGK ; STR ; urology ; X chromosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The human phosphoglycerate kinase (PGK) gene is located within Xq11-Xq13, a region implicated in genitourinary diseases including: prostate cancer, androgen insensitivity, perineal hypospadias, and other genetic abnormalities. The PGK gene and the androgen receptor gene are in linkage disequilibrium. PGK has been mapped extensively for nuclease-sensitive sites, methylation sites, and flanking DNA sequences. A PGK-associated BstXI polymorphism has been used to determine clonality of neoplastic tissues. Using fluorescent PCR product analysis and DNA sequencing, we discovered that a short tandem repeat (STR) in the 3′ flanking region of the PGK gene is polymorphic. Among 231 individuals, there were nine distinct alleles, including eight based on variations in the number of TATC repeats. The PGK STR demonstrated hemizygosity, consistent with its X-chromosomal location and with an absence of cross-hybridizing autosomal homologs. The polymorphic PGK STR shows promise for rapid investigation of neoplastic clonality, for personal identification, and for studies of inherited predisposition to urologic disorders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006908624757
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  • 10
    Electronic Resource
    Electronic Resource
    Über den Reaktionsverlauf zwischen aromatischen Aldehyden und Diacetylmonoxim bei Gegenwart von starker Salzsäure (1915)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 48 (1915), S. 897-905 
    Details
    ISSN: 0365-9496
    Keywords: Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.191504801121
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