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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 84 (1992), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cloning is crucial in horticulture and forestry for commercial production of plants and in genetic improvement programs. However, inability to induce adventitious root formation (rooting) often limits cloning of woody plants by cuttage or tissue culture. To improve cloning, past physiological and biochemical studies of rooting have largely been aimed at understanding controls by researching post-translational processes. Still, the sought-for controls of rooting remain obscure. However, those controls may be discovered by searching for and studying direct genetic effects, using current molecular genetic technologies and new plant materials (e.g., non-rooting mutants, normally rootless plants). In particular, applying molecular genetic technologies in studies at the transcriptional and translational levels may soon reveal the controls of rooting. We review strategies for achieving that goal.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 86 (1992), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We tested the hypothesis that, during propagation, the basal stem of Pinus banksiana Lamb, cuttings contains a nonauxin endogenous root-forming stimulus (ERS) whose effects are not replaced by auxin treatment; i.e., that auxin treatment of cuttings and surgical removal of their basal stems during propagation act additively on rooting, without significant interaction, as determined by analyses of variance. We studied the effects of ERS by severing the original basal (1 cm) stem from cuttings at days 3, 6 and 9 of a 30-day period of propagation. To study treatment interaction, we applied the auxin N-phenyl indolyl-3-butyramide to some surgically treated cuttings. Results for all variables (proportion of rooted cuttings, number of roots per cutting and fresh weight of basal stem plus roots, if any) indicated that removing the basal stem reduced rooting and that auxin treatment increased rooting, without significant treatment interaction. The results thus supported the hypothesis that P. banksiana seedling cuttings contain ERS. However, neither the present nor previous tests have shown that ERS is a chemical stimulus rather than, for example, a biophysical or anatomical one.
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  • 3
    ISSN: 1573-5028
    Keywords: bark storage protein ; gene clustering ; RFLP ; systemic response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two wound-inducible cDNAs from poplar leaves show sequence identity to vegetative storage proteins (VSP) that accumulate seasonally in poplar bark tissues. We have compared the genomic organization, cDNA sequences and expression of the genes encoding the wound-inducible cDNAs (win4) with that of a bark VSP (called bark storage protein, or BSP). There appear to be several win4 genes in the poplar genome which segregate as a single locus and are therefore likely to be clustered. The same is true of the BSP genes. The win4 locus is linked (map distance of 5 cM) to the BSP locus, consistent with a common evolutionary origin of the genes. A near full-length win4 cDNA shows 75% sequence identity to BSP cDNAs. Both win4 and BSP are systemically wound-inducible; win4 transcripts accumulate in leaves and stems, whereas BSP transcripts accumulate almost exclusively in stems. A phloem transport-dependent signaling mechanism appears to be involved in systemic win4 expression after wounding. In contrast to BSP gene expression, win4 genes are not expressed in response to short day conditions. The data indicate win4 and BSP genes are differentially regulated, and their products may play important roles in the storage and reallocation of nitrogen in perennial plants.
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  • 4
    Publication Date: 2005-04-01
    Description: Rooting of hardwood cuttings is under strong genetic control, although genotype × environment interactions affect selection of promising genotypes. Our objectives were (1) to assess the variation in rooting ability among 21 Populus clones and (2) to examine genotype × environment interactions to refine clonal recommendations. The clones belonged to five genomic groups ((Populus trichocarpa Torr. & Gray × Populus deltoides Bartr. ex Marsh.) × P. deltoides 'BC'; P. deltoides 'D'; P. deltoides × Populus maximowiczii A. Henry 'DM'; P. deltoides × Populus nigra L. 'DN'; and P. nigra × P. maximowiczii 'NM'). Cuttings, 20 cm long, were planted in Iowa and Minnesota in randomized complete blocks at 1.2 m × 2.4 m spacing, across three planting dates during 2001 and 2002. We measured nine belowground and aboveground traits from harvested cuttings after 14 days of growth. Percent rooting ranged from 22% to 86%. Broad-sense heritability for root and top dry mass ranged from 0.09 to 0.11 and 0.31 to 0.38, respectively. There were genotype × environment interactions for most traits, with belowground growing degree-days accounting for 〉54% of environmental variation. Clonal rooting was stable, except at Westport, Minnesota, during 2002, when root growth was relatively poor.
    Print ISSN: 0045-5067
    Electronic ISSN: 1208-6037
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 5
    Publication Date: 1994-08-01
    Description: We conducted a study to determine whether genotypic and phenotypic covariances among important traits differed between collections of black cottonwood (Populustrichocarpa Torn & Gray) from British Columbia and northern Idaho. We also determined how differences in estimated covariances could affect various multiple-trait clonal selection strategies. The experimental design consisted of 166 clones from British Columbia and northern Idaho intermixed at random in two replications of three-tree row plots. Measurements made during the second growing season included tree height, frequency and number of sylleptic branches, number of leaves, and the lengths and areas of leaf plastochron index 5, 10, and 15 leaves at the end of the growing season. The severity and extent of Melampsora leaf rust and Septoria leaf spot were also evaluated. We found high heritability for tree height, phenology, leaf morphology, and resistance to damaging agents. However, no advantage to using leaf morphology to improve genetic selection for tree height was demonstrable. Restricted selection indices had high potential utility for controlling increases in damaging agents. However, the relation between tree height and leaf diseases varied between the British Columbia and northern Idaho collections of black cottonwood, and between black cottonwood and an adjacent experimental population of balsam poplar (Populusbalsamifera L.). Such population-related variation in intertrait covariances suggests that selection strategies involving disease resistance may need to be evaluated on an individual population basis.
    Print ISSN: 0045-5067
    Electronic ISSN: 1208-6037
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 6
    Publication Date: 1992-04-01
    Description: Cuttings were collected from balsam poplars (Populusbalsamifera L.) representing 21 populations in Minnesota, Wisconsin, and Michigan, and a replicated nursery trial containing 153 clones was established. Tree height, phenology, leaf morphology, and resistance to damaging agents were evaluated during the second growing season, and genetic and phenotypic variances and covariances among traits were estimated. Clones differed significantly in all traits. However, populations differed only in tree height, number of leaves produced after August 31, bud-set date, number of sylleptic branches (i.e., branches that arise from buds the same year that the buds formed), and severity of Septoria leaf spot symptoms. Tree height was positively correlated with number of leaves, bud-set date, most measures of leaf size, and resistance to Melampsora leaf rust. Predicted responses to index selection for 2nd-year tree height using leaf area or leaf length as secondary selection criteria were not significantly greater than predicted response to univariate selection for tree height alone. However, results supported use of restricted selection indices to curb increased susceptibility to damaging agents that would otherwise result from selection for tree height.
    Print ISSN: 0045-5067
    Electronic ISSN: 1208-6037
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 7
    Publication Date: 1994-12-01
    Description: Hybrid poplar clone NC–5339 (Populusalba × Populusgrandidentata cv. Crandon) was genetically modified for glyphosate (N-(phosphonomethyl)glycine) tolerance by Agrobacterium-mediated transformation with genetic constructs (pPMG 85/587 and pCGN 1107) that included the mutant aroA gene for 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase (EC 2.5.1.19) and the neomycin phosphotransferase selectable marker gene. pCGN 1107 also harbored the coding sequence for a chloroplast transit peptide and the CaMV 35S promoter fused to the mutant aroA gene. Transformants were selected for kanamycin tolerance, and integration of the aroA gene was verified by Southern blot analysis. Cuttings of NC-5339 and the derived transformants were rooted and grown in glasshouses at separate locations, with maximum photosynthetic photon flux density of 1600 and 750 μmol•m−2•s−1. Productivity was assessed by growth studies and photosynthesis measurements at both locations. Glyphosate tolerance was tested by (i) measurement of chlorophyll concentration in herbicide-treated leaf discs and (ii) whole-plant spray tests. Plants transformed with construct pCGN 1107 were the most herbicide tolerant. Perhaps high-level expression of the aroA gene by the CaMV 35S promoter, transport of mutant EPSP synthase into the chloroplasts, or both facilitated glyphosate tolerance. Plants grown at higher photosynthetic photon flux densities (1600 vs. 750 μmol•m−2•s−1) had significantly higher maximum net photosynthesis (19.8 vs. 16.2 μmol•m−2•s−1) and more biomass accumulation (47.6 vs. 33.7 g). However, there were no significant differences between NC-5339 and transformants within location for net photosynthesis or any growth parameter. Genetic modification of hybrid poplar NC-5339 for glyphosate tolerance did not adversely affect plant productivity at either location.
    Print ISSN: 0045-5067
    Electronic ISSN: 1208-6037
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 8
    Publication Date: 1998-04-01
    Print ISSN: 0961-9534
    Electronic ISSN: 1873-2909
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Process Engineering, Biotechnology, Nutrition Technology
    Published by Elsevier
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