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  • 1
    Electronic Resource
    Electronic Resource
    Micropropagation of floribunda, ground cover and miniature roses (1989)
    Springer
    Plant cell, tissue and organ culture 19 (1989), S. 55-64 
    Details
    ISSN: 1573-5044
    Keywords: rose ; meristem culture ; micropropagation ; root production ; long-term cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultures were initiated from meristems (0.5 mm) of the rose cultivar Queen Elizabeth (floribunda) and from both shoot-tips and nodal explants (3–5 mm) of cultivars Sunburst Red, Toy Clown (miniatures) and Fiona (ground cover). Average proliferations of 5.0, 3.1, 1.3 and 2.5 shoots were obtained per culture cycle respectively on Murashige & Skoog (MS) medium with BA (1.0 mg l-1), NAA (0.1 mg l-1) and GA3 (0.1 mg l-1). With cv. Fiona, the proliferation rate was more than doubled by removal of the shoot apex. The rate of proliferation of cv. Queen Elizabeth was significantly increased by using long shoots (〉2 cm in length) and by re-culturing shoots to fresh medium every 3 weeks. In vitro rooting percentage with cv. Queen Elizabeth was enhanced by using long shoots (〉2 cm) and by dilution of MS medium to 1/4 strength. Transfer of shoots for direct rooting in compost was significantly improved by pre-culturing shoots for two weeks in vitro in media containing IAA, and by the use of sorbarods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037776
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  • 2
    Electronic Resource
    Electronic Resource
    Callus production, suspension culture and in vitro alkaloid yields of Ephedra (1993)
    Springer
    Plant cell, tissue and organ culture 34 (1993), S. 149-155 
    Details
    ISSN: 1573-5044
    Keywords: alkaloids ; ephedrine ; in vitro culture ; pseudoephedrine ; Ephedra ; phytohormones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of a range of plant growth regulators on callus production in various Ephedra species were examined. Species examined were E. andina, E. distachya, E. equisitina, E. fragilis var, camplyopoda, E. gerardiana, E. intermedia, E. major ssp procera, E. minima and E. saxatilis. All species produced callus on modified MS medium supplemented with 0.25 μM kinetin and 5.0 μM 2,4-dichlorophenoxyacetic acid or 1-naphthaleneacetic acid. Neither indole-3-acetic acid nor 3-indolebutyric acid induced significant callus formation but the latter maintained growth of established callus cultures in several species. Suspension cultures of several species were established in MS medium supplemented with 0.25 μM kinetin and 5.0 μM 2,4-dichlorophenoxyacetic acid or 1-naphthaleneacetic acid. Sustained fresh weight doubling times of 70±7h were recorded for cell suspension cultures of E. andina grown in a semi-continous air-lift bubble bioreactor and a minimum doubling time of 56 h was recorded for E. andina in batch culture. It also proved possible to immobilise E. andina batch cultures in sodium alginate beads. Neither parent plants or in vitro cultures of E. distachya, E. fragilis or E. saxatilis produced alkaloids. Trace quantities of 1-ephedrine and trace-0.14% dwt d-pseudoephedrine were produced by in vitro cultures of other species. The ability to produce alkaloid diminished to zero with successive subcultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036095
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  • 3
    Electronic Resource
    Electronic Resource
    Determination of some metal ions using lichen-modified carbon paste electrodes (1991)
    Weinheim : Wiley-Blackwell
    Electroanalysis 3 (1991), S. 331-336 
    Details
    ISSN: 1040-0397
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Lichens have long been used as biomonitors of environmental pollution. We therefore investigated the application of lichen-modified carbon paste electrodes (CPEs) for the determination of lead(II) and copper(II) using differential pulse anodic stripping voltammetry. These electrochemical biosensors incorporate the biological selectivity of lichen species such as Cladonia portentosa and Lobaria pulmonaria, and the genus Roccella, with the sensitivity of electrochemical detection. As such, they may offer new reactivity patterns that could be exploited in the determination of trace metal ions in environmental samples and in speciation studies. The voltammetric responses were evaluated with respect to pH of accumulation (carried out under open circuit conditions), pH of electrolyte, solution, metal ion concentration, percentage lichen loading in the carbon paste, interferences, and surface renewal.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elan.1140030414
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