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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 37 (1989), S. 659-662 
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 25 (1978), S. 0 
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Incorporation of 14C-labeled biopterin into Crithidia fasciculata was inhibited by 1 mM colchicine or lumicolchicine. These substances do not penetrate the cell membrane, hence they cannot interact with the subpellicular microtubules. In view of this, interference of colchicine with biopterin transport must occur on the outer surface of the cell membrane. Binding of colchicine to Crithidia was not temperature-dependent and did not exhibit saturation kinetics. These facts exclude a binding as in the case of tubulin, or similar proteins which may be present in the membrane. The results suggest an inhibition reflecting steric hindrance of the biopterin carrier system.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 171 (1992), S. 325-341 
    ISSN: 1432-1351
    Keywords: Phonotaxis ; Crickets ; Allatectomy ; Juvenile Hormone and 20-OH-ecdysone titers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The role of Juvenile Hormone III in phonotaxis, mating behavior and egg production of cricket females from Gryllus campestris, Gryllus bimaculatus, Teleogryllus commodus, and Acheta domesticus was investigated. 1. Phonotaxis of crickets was measured on a Kramer spherical treadmill by means of an infrared system which allowed free walking at any speed and direction. Crickets were allatectomized during the adult or last nymphal stage, and the controls sham-operated. On each cricket, 2 to 9 phonotactic tests were performed during a period of 12 to 51 days; the allatectomized nymphs were tested as adults. 2. All females deprived of their corpora allata during the adult stage continued to exhibit phonotaxis, whereas females operated as nymphs developed and maintained phonotaxis (Tables 1–4). Some of these females allatectomized or sham-operated during the adult or nymphal stage exhibited correct phonotactic behavior as well as undirected walking. 3. At the end of the test series, all allatectomized females were found to be devoid of Juvenile Hormone, whereas the controls varied according to age from 0.2 to 2.0 pmol/10 μl. The individual ecdysteroid levels differed unpredictably in allatectomized and control females (0.02 to 6.4 pmol/10 μl), and no correlation was found between the number of eggs produced by allatectomized females and their ecdysteroid titer. 4. All allatectomized females displayed sexual receptivity. 5. Adults allatectomized during the last nymphal instar were still able as adults to develop single eggs in G. campestris and G. bimaculatus, while females of T. commodus did produce and store moderate numbers. However, in A. domesticus no eggs were produced, and terminal oocytes had reached 0.5 to 0.7 mm in length, which is the previtellogenic size of terminal oocytes in normal females at the age of one to two days.
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  • 4
    ISSN: 1573-1561
    Keywords: Attractant ; Cajanus cajan ; EAG ; Helicoverpa armigera ; kairomone ; Lepidoptera ; Noctuidae ; oviposition stimulant ; pheromone ; pigeonpea ; sesquiterpene ; β-caryophyllene ; α-humulene ; α-guajene ; α-muurolene ; γ-muurolene ; α-bulnesene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract FemaleH. armigera moths are highly attracted by a steam distillate from pigeonpea plants, one of their main hosts. A mixture of six compounds, all sesquiterpenes (β-caryophyllene,α-humulene,α-guajene,α-muurolene,γ-muurolene, andα-bulnesene), mixed in the proportions as found in the steam distillate, elicited the same behavioral responses (oriented upwind flights and contacts with the odor source) as the steam distillate. Onlyα-bulnesene was attractive by itself, but still less than the whole mixture. In addition, the sesquiterpene mixture acts as an oviposition stimulant. Both behavioral responses, orientation and oviposition, are concentration dependent. Electrophysiological recordings from female and male antennae (EAG) showed the same qualitative and quantitative responses to each of the compounds of the sesquiterpene mixture. The EAG responses to the original steam distillate were higher and similar to chickpea kairomonal components, which were also tested. The pigeonpea sesquiterpene mixture and its individual components elicited weak EAG responses only. The response of the male antenna to female-produced pheromone components was in the same range as the pigeonpea steam distillate.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 230 (1983), S. 49-55 
    ISSN: 1432-0878
    Keywords: Endocrine system ; Corpora cardiaca ; Corpora allata ; Honey bee castes ; Neurosecretion ; Postembryonic development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The endocrine system of female honey bee larvae has been studied through postembryonic development with histological and autoradiographic techniques. During larval development, brain and retrocerebral complex proceed from immature cells to an active endocrine system. Caste-specific retardation occurs in the worker during this process. In the developing queen, the differentiation of the neurosecretory cells (NSC) and the outgrowth of their axons occurs from the second instar onward and is nearly completed in the fourth, whereas in the worker larva these processes are delayed by more than one instar. In the queen, RNA synthesis starts in the NSC at the end of the third instar and in the worker at the fifth instar. Stainable neurosecretory material is present only in fifth instar queen larvae. The queen's corpora cardiaca become active at the end of the fourth, those of the worker in the fifth instar. In the corpora allata (CA), nuclei undergo several phases of endomitosis. These phases of polyploidization end at the beginning (queen) or at the end (worker) of the fifth instar respectively. CA volume in the queen is twice that of a worker at its height at the end of larval development. These data demonstrate a caste-specific maturation of the endocrine organs which results in differences in hormone titres.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 237 (1968), S. 50-59 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung 2,4-Diamino-6,7-dimethyl-pteridin ist ein sehr potentes Diureticum, das eine starke Natriumausscheidung bei gleichzeitiger Kaliumretention bewirkt. Mit Hilfe einer speziellen Methode für vergleichende analytische Chromatographie konnte gezeigt werden, daß diese Verbindung von der Ratte quantitativ umgewandelt wird. Als Hauptmetabolit erscheint 2,4-Diamino-7-methyl-pteridin-carbonsäure-(6) im Harn. Die Struktur der ausgeschiedenen Verbindung wurde durch UV-, NMR- und Massen spektren sowie durch Synthese bewiesen. Gleichzeitig mit dem Auftreten des Metaboliten sinkt die im Harn ausgeschiedene Harnsäuremenge drastisch ab. Eine Veränderung des Serumharnsäurespiegels konnte nicht festgestellt werden. Die beobachtete Wirkung bedeutet zugleich eine Inaktivierung des Diureticums: verfüttert man den Metaboliten, so findet man keine vergleichbare diuretische Wirkung und die Harnsäure ausscheidung der Ratte ändert sich nicht.
    Notes: Summary 2,4-Diamino-6,7-dimethyl-pteridine is a potent diuretic agent, exhibiting a marked sodium excretion as well as a reduction of the potassium output. By means of a special analytical method for comparative chromatography, a complete metabolic transformation of this substance by rats could be shown. As main metabolic product 2,4-diamino-7-methyl-pteridine-carboxylic-acid-(6) was isolated from urine. Unequivocal evidence for the chemical structure of the excreted compound is presented by UV-, NMR-, and mass spectrometry, as well as by chemical synthesis. In addition to the appearance of this metabolite, a simultaneous strong decrease of normal uric acid excretion was found. The conversion of the diuretic agent into the excreted metabolite obviously implies a biological inactivation: the metabolite being administered to rats, no diuretic acitivity is observed and uric acid excretion is not affected.
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  • 7
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A complex mixture of O-(β-hydroxyethyl)-rutosides was separated by Sephadex LH 20 chromatography. The isolated compounds could be identified as their permethyl derivatives. The quatitative distribution of single compounds was determined by selected ion monitoring of the trimethylsilylated mixture.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0878
    Keywords: Key words: Azadirachtin A – Prothoracic gland – Corpus allatum – Corpus cardiacum – Ring complex –Lucilia cuprina (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. This study investigated the effect of azadirachtin A (AZ A) on the ultrastructure of the endocrine glands (the prothoracic gland, the corpus allatum, and the corpus cardiacum) responsible for controlling moulting and ecdysis in Lucilia cuprina larvae. All constituent endocrine glands within the ring complex exhibited ultrastructural change after exposure of larvae to AZ A in the diet. The effects on the nucleus were the most noticeable and included: (1) crenulation of nuclear shape, (2) clumping of heterochromatin, and (3) pyknosis. The degenerative changes to the nuclei appeared to precede changes within the cytoplasm. It was concluded that the degeneration of all three endocrine glands within the ring complex would contribute to a generalised disruption of neuroendocrine function.
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  • 9
    ISSN: 1432-136X
    Keywords: Caste differentiation ; Juvenile hormone III content ; Ecdysteroid titer ; Stingless bee ; Scaptotrigona postica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Juvenile hormone III content and ecdysteroid titer were analyzed for larval and pupal development of the stingless bee,Scaptotrigona postica depilis. Castespecific differences in juvenile hormone III content were detected at three developmental phases: at the transition from the fourth to the fifth larval stadium, in the spinning phase of the fifth larval stadium, and shortly after the imaginal moult. During the fifth larval stadium, juvenile hormone content closely reflects corpora allata activity. Juvenile hormone synthesis may thus be responsible for the elevated hormone titer in spinning-phase queen larvae, a phase of known sensitivity for induction of queen characters by exogenous juvenile hormone. For ecdysteroids, two phases of caste-specific differences were found: in the pre-pupal phase, and shortly after the imaginal moult. In both periods the titer in queens is distinctly higher compared to workers.
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  • 10
    ISSN: 1432-0878
    Keywords: Azadirachtin A ; Prothoracic gland ; Corpus allatum ; Corpus cardiacum ; Ring complex ; Lucilia cuprina (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract This study investigated the effect of azadirachtin A (AZA) on the ultrastructure of the endocrine glands (the prothoracic gland, the corpus allatum, and the corpus cardiacum) responsible for controlling moulting and ecdysis in Lucilia cuprina larvae. All constituent endocrine glands within the ring complex exhibited ultrastructural change after exposure of larvae to AZ A in the diet. The effects on the nucleus were the most noticeable and included: (1) crenulation of nuclear shape, (2) clumping of heterochromatin, and (3) pyknosis. The degenerative changes to the nuclei appeared to precede changes within the cytoplasm. It was concluded that the degeneration of all three endocrine glands within the ring complex would contribute to a generalised disruption of neuroendocrine function.
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