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  • 1
    Electronic Resource
    Electronic Resource
    Separation of capsaicin from phenylpropanoid compounds by high-performance liquid chromatography to determine the biosynthetic status of cells and tissues of Capsicum frutescens Mill. in vivo and in vitro (1992)
    s.l. : American Chemical Society
    Journal of agricultural and food chemistry 40 (1992), S. 2461-2463 
    Details
    ISSN: 1520-5118
    Source: ACS Legacy Archives
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/jf00024a024
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  • 2
    Electronic Resource
    Electronic Resource
    Control of Ecdysterone Biogenesis in Tissue Cultures of Trianthema portulacastrum (1979)
    s.l. : American Chemical Society
    Journal of natural products 42 (1979), S. 152-158 
    Details
    ISSN: 1520-6025
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/np50002a003
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  • 3
    Electronic Resource
    Electronic Resource
    Influence of Polyamines on Growth of Hairy Root Cultures of Witloof Chicory (Cichorium intybus L. cv. Lucknow Local) and Formation of Coumarins (1999)
    Springer
    Journal of plant growth regulation 18 (1999), S. 33-37 
    Details
    ISSN: 1435-8107
    Keywords: Key Words.Cichorium intybus L. cv. Lucknow local—Hairy root cultures—Polyamines—Coumarins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. The effect of polyamines (putrescine, spermidine, and spermine) was examined for growth and production of two coumarins, esculetin and esculin, in the hairy roots of chicory (Cichorium intybus L. cv. Lucknow local). Of the polyamines administered, 1.5 mm putrescine alone resulted in a 2.3-fold higher increase in the growth of hairy roots as well as in the production of esculetin and esculin, which was 3.37 times more than that of the control on day 21. The endogenous level of conjugated putrescine was more than fivefold that of free putrescine levels in untreated samples. The production of esculetin and esculin in hairy root cultures strictly correlated with growth in all of the treatments. Putrescine at 1.5 mm resulted in a greater length of primary root (18.29 ± 1.37 cm) compared with the control (10.96 ± 0.82 cm) and more secondary and tertiary roots. This study also provides insight into the morphogenetic changes that occur in roots in response to the external supply of polyamines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007043
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  • 4
    Electronic Resource
    Electronic Resource
    Enhancement of anthocyanin production in callus cultures ofDaucus canota L. under the influence of fungal elicitors (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 227-231 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Aqueous extracts of fungal biomass ofAspergillus niger, A. flavus, Penicillium notatum, Fusarium oxysporum and the filtrates of their culture media were analysed for elicitation capability to enhance anthocyanin production in callus cultures ofDaucus carota. The mycelial extract ofA. flavus at the 2.5% level gave maximum elicitation, which resulted in a two-fold increase in anthocyanin with maximal productivity of 23.7% on a dry weight basis. Whereas the media filtrates ofA. flavus induced a 1.25-fold increase in anthocyanin production with a yield of 20.6% on a dry weight basis,P. notatum andF. oxysporum were not as effective asA. flavus orA. niger. The contact time and the concentration required for maximal elicitation of anthocyanin differed with the type of elicitor used. Qualitative analysis of anthocyanins revealed the presence of cyanidin glycosides in control and elicited cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00902721
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  • 5
    Electronic Resource
    Electronic Resource
    Phycocyanin, a new elicitor for capsaicin and anthocyanin accumulation in plant cell cultures (1996)
    Springer
    Applied microbiology and biotechnology 46 (1996), S. 619-621 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract   Elicitors of both fungal and bacterial origin that is, polysaccharides, proteins and fatty acids, are widely used for enhancement of secondary metabolites in plant cell cultures. In the present study, phycocyanin – a natural blue pigment that is the major light-harvesting biliprotein in the blue-green alga Spirulina platensis– was used as an elicitor to enhance the accumulation of capsaicin and anthocyanin in Capsicum frutescens and Daucus carota cell cultures respectively. Phycocyanin at 0.3, 0.6 and 1.2 mg% in capsicum cell cultures elicited a more than two-fold increase in capsaicin content with maximum productivity of 192 μg/g fresh weight. Similarly in Daucus carota cell cultures a two-fold increase in anthocyanin content was obtained at 0.3 mg% with a maximum productivity of 24.8 mg% on a dry-weight basis. In both the systems, phycocyanin showed an early elicitation of secondary metabolites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050871
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  • 6
    Electronic Resource
    Electronic Resource
    Enhancement of anthocyanin production in callus cultures of Daucus carota L. under the influence of fungal elicitors (1994)
    Springer
    Applied microbiology and biotechnology 42 (1994), S. 227-231 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Aqueous extracts of fungal biomass of Aspergillus niger, A. flavus, Penicillium notatum, Fusarium oxysporum and the filtrates of their culture media were analysed for elicitation capability to enhance anthocyanin production in callus cultures of Daucus carota. The mycelial extract of A. flavus at the 2.5% level gave maximum elicitation, which resulted in a two-fold increase in anthocyanin with maximal productivity of 23.7% on a dry weight basis. Whereas the media filtrates of A. flavus induced a 1.25-fold increase in anthocyanin production with a yield of 20.6% on a dry weight basis, P. notatum and F. oxysporum were not as effective as A. flavus or A. niger. The contact time and the concentration required for maximal elicitation of anthocyanin differed with the type of elicitor used. Qualitative analysis of anthocyanins revealed the presence of cyanidin glycosides in control and elicited cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00902721
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  • 7
    Electronic Resource
    Electronic Resource
    Regulation of nicotine biogenesis 2: Increased production of nicotine by urea in tobacco tissue cultures (1981)
    Springer
    Cellular and molecular life sciences 37 (1981), S. 1143-1144 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The floral bud callus ofNicotiana tabacum L. was successfully grown on 10 mM urea as sole nitrogen source. Nicotine content in the urea-treated tissues was 3.5 times higher than in the control on the basis of dry weight, and 2.5-fold higher per culture. Contents of ornithine, citrulline and arginine, urea cycle intermediates, and known precursors of nicotine, were higher in urea-treated callus. The implications of the results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01989884
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  • 8
    Electronic Resource
    Electronic Resource
    In vitro multiplication of Vanilla planifolia using axillary bud explants (1997)
    Springer
    Plant cell reports 16 (1997), S. 490-494 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsVanilla planifolia ; Axillary bud ; Liquid media ; Multiple shoots ; Plantlets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A clonal propagation method has been developed for efficient multiplication of Vanilla planifolia. Multiple shoots were developed from axillary bud explants using semi-solid Murashige and Skoog (MS) medium supplemented with N6-benzyladenine (BA, 2 mg l–1) and α-naphthaleneacetic acid (NAA, 1 mg l–1). The multiple shoots were transferred to agitated liquid MS medium with BA at 1 mg l–1 and NAA at 0.5 mg l–1 for 2–3 weeks, and subsequently cultured on semi-solid medium. Using this method, an average of 42 shoots were obtained from a single axillary bud explant over a period of 134 days. Use of an intervening liquid medium has been found to enhance multiplication of shoots in V. planifolia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092772
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  • 9
    Electronic Resource
    Electronic Resource
    Clonal multiplication of Gardenia jasminoides Ellis through axillary bud culture (1993)
    Springer
    Plant cell reports 13 (1993), S. 59-62 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An efficient clonal multiplication system was developed for in vitro propagation of crocin — producing Gardenia jasminoides Ellis plants. Murashige and Skoog's (MS) medium containing 6-benzylaminopurine (BAP 1 mg l−1) and indole-3-butyric acid (IBA 1 mg l−1) resulted in multiple shoot initiation at the rate of 21 shoots per explant in 60 d of culture. Transfer of the microshoots into liquid MS medium supplemented with BAP (5 mg l−1) with two subcultures of 15 d duration in the same medium resulted in 400 ± 25 shoots per explant. Efficient rooting was achieved in MS medium supplemented with α-naphthaleneacetic acid (5 mg l−1). The in vitro raised plants were hardened in a greenhouse and transplanted to the field successfully. The method described will be useful for rapid multiplication of Gardenia for commercial exploitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232317
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  • 10
    Electronic Resource
    Electronic Resource
    In vitro multiplication ofVanilla planifolia using axillary bud explants (1997)
    Springer
    Plant cell reports 16 (1997), S. 490-494 
    Details
    ISSN: 1432-203X
    Keywords: Vanilla planifolia ; Axillary bud ; Liquid media ; Multiple shoots ; Plantlets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A clonal propagation method has been developed for efficient multiplication ofVanilla planifolia. Multiple shoots were developed from axillary bud explants using semi-solid Murashige and Skoog (MS) medium supplemented with N6-benzyladenine (BA, 2 mg l−1) and α-naphthaleneacetic acid (NAA, 1 mg l−1). The multiple shoots were transferred to agitated liquid MS medium with BA at 1 mg l−1 and NAA at 0.5 mg l−1 for 2–3 weeks, and subsequently cultured on semi-solid medium. Using this method, an average of 42 shoots were obtained from a single axillary bud explant over a period of 134 days. Use of an intervening liquid medium has been found to enhance multiplication of shoots inV. planifolia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092772
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