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  • 1
    Electronic Resource
    Electronic Resource
    Induction of division and differentiation of somatic embryos in the leaf epidermis of Gaillardia picta (1992)
    Springer
    Plant cell reports 10 (1992), S. 599-603 
    Details
    ISSN: 1432-203X
    Keywords: Epidermis ; monolayer culture ; morphogenesis ; somatic embryos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm x 3 mm were cultured on B5 basal medium supplemented with various growth regulators such as naphthaleneacetic acid or indolebutyric acid and benzylaminopurine or kinetin. Within 12 h of culture the epidermal cells showed receding of cytoplasm from the walls. After 48 h of incubation 3 or 4 localized zones, each consisting of 3–8 cells that accumulated cytoplasm and stained densely, were observed. Mitotic divisions occurred in these zones on day 3 of culture and localized masses of callus were observed in 95% of the cultures after 10 days. In another 5 days, the callus differentiated somatic embryos or roots, depending on the growth regulators and their concentration in the medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232378
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  • 2
    Electronic Resource
    Electronic Resource
    Somatic embryogenesis and regeneration of plants in the bamboo Dendrocalamus strictus (1985)
    Springer
    Plant cell reports 4 (1985), S. 191-194 
    Details
    ISSN: 1432-203X
    Keywords: Bamboo ; Dendrocalamus strictus ; Regeneration ; Somatic Embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryogenesis leading to plant regeneration has been achieved in the bamboo, Dendrocalamus strictus, by culturing seeds (caryopses) on B5 basal medium supplemented with 2,4-dichlorophenoxyacetic acid. Callus cultures obtained from the embryonal end of the seeds differentiated chlorophyllous embryoids. On transfer to a germination medium (B5 liquid, sucrose, indolebutyric acid, and ∝ -naphthaleneacetic acid) 40% of the embryoids developed into plantlets. Further development of the plantlets occured on B5 liquid medium (half strength) + sucrose (1%) + IBA (5 × 10−7M) + NAA (10−7M).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269286
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    Paper (German National Licenses)
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