ALBERT

Description: A major bottleneck in identifying therapies to control citrus greening and other devastating plant diseases caused by fastidious pathogens is our inability to culture the pathogens in defined media or axenic cultures. As such, conventional approaches for antimicrobial evaluation (genetic or chemical) rely on time-consuming, low-throughput and inherently variable whole-plant assays. Here, we report that plant hairy roots support the growth of fastidious pathogens like Candidatus Liberibacter spp., the presumptive causal agents of citrus greening, potato zebra chip and tomato vein greening diseases. Importantly, we leverage the microbial hairy roots for rapid, reproducible efficacy screening of multiple therapies. We identify six antimicrobial peptides, two plant immune regulators and eight chemicals which inhibit Candidatus Liberibacter spp. in plant tissues. The antimicrobials, either singly or in combination, can be used as near- and long-term therapies to control citrus greening, potato zebra chip and tomato vein greening diseases.

Description: This paper describes simultaneous optical measurements of a sub-scale helicopter rotor in the U.S. Army Hover Chamber at NASA Ames Research Center. The measurements included thermal imaging of the rotor blades to detect boundary layer transition; retro-reflective background-oriented schlieren (RBOS) to visualize vortices; and stereo photogrammetry to measure displacements of the rotor blades, to compute spatial coordinates of the vortices from the RBOS data, and to map the thermal imaging data to a three-dimensional surface grid. The test also included an exploratory effort to measure flow near the rotor tip by tomographic particle image velocimetry (tomo PIV)an effort that yielded valuable experience but little data. The thermal imaging was accomplished using an image-derotation method that allowed long integration times without image blur. By mapping the thermal image data to a surface grid it was possible to accurately locate transition in spatial coordinates along the length of the rotor blade.

Description: The initial roll-up of a tip vortex trailing from a model-scale, hovering rotor was measured using particle image velocimetry. The unique feature of the measurements was that a microscope was attached to the camera to allow much higher spatial resolution than hitherto possible. This also posed some unique challenges. In particular, the existing methodologies to correct for aperiodicity in the tip vortex locations could not be easily extended to the present measurements. The difficulty stemmed from the inability to accurately determine the vortex center, which is a prerequisite for the correction procedure. A new method is proposed for determining the vortex center, as well as the vortex core properties, using a least-squares fit approach. This approach has the obvious advantage that the properties are derived from not just a few points near the vortex core, but from a much larger area of flow measurements. Results clearly demonstrate the advantage in the form of reduced variation in the estimated core properties, and also the self-consistent results obtained using three different aperiodicity correction methods.

Description: The advancement of flow measurement techniques continues to extend experimental boundaries and thus significantly contributes to improving our understanding of both basic and applied aerodynamics. This is particularly apparent in the case of particle image velocimetry (PIV), where its application has furthered the existing knowledge in several areas of helicopter rotor aerodynamics. The complex nature of helicopter rotor flows presents unique challenges to experimentalists, including transonic flow, concentrated vortices and dynamic stall. To illustrate the impact of the technological advancements on the way helicopter aerodynamics is studied today, the development of PIV since the early nineties of the last century is reviewed and some recent PIV applications are described. Using examples of main rotor wakes, dynamic stall and flow control investigations, the capabilities of largescale, timeresolved and volumetric PIV are summarized.

Description: Downwash and outwash characteristics of a model-scale tandem-rotor system in the presence of the ground were analyzed by identifying and understanding the physical mechanisms contributing to the observed flow field behavior. A building block approach was followed in simplifying the problem, separating the effects of the fuselage, effects of one rotor on the other, etc. Flow field velocities were acquired in a vertical plane at four aircraft azimuths of a small-scale tandem rotor system using the particle image velocimetry (PIV) technique for radial distances up to 4 times the rotor diameter. Results were compared against full-scale CH-47D measurements. Excellent correlation was found between the small- and full-scale mean flow fields (after appropriate normalization using rotor and wall jet parameters). Following the scalability analysis, the effect of rotor height on the outwash was also studied. Close to the aircraft, an increase in rotor height above ground decreased the outwash velocity at all aircraft azimuths. However, farther away, the longitudinal and lateral axes of the aircraft showed increasing and decreasing outwash velocities, respectively, with increasing rotor height. Measurements also indicated the presence of large-scale (of the size of the rotor height) shear-layer vortical structures along the ground that could be the source of low-frequency (approximately 1 Hz) flow variation observed in the full-scale measurements. Flow visualization studies and PIV measurements were also made on jets of different sizes to complement the observations made on rotors wherever possible. Baseline rotor measurements were made out-of-ground effect to understand the nature of inflow distribution for realistic rotor configurations and their modified characteristics in the presence of ground. Lastly, a feasibility study on applying high-fidelity CFD simulations for outwash study was conducted using Helios to model an isolated rotor configuration IGE at full-scale Reynolds number. The results were encouraging and demonstrated the practical challenges associated with predicting rotor outwash.

Description: Downwash and outwash characteristics of a model-scale tandem-rotor system in the presence of the ground were analyzed by identifying and understanding the physical mechanisms contributing to the observed flow field behavior. A building block approach was followed in simplifying the problem, separating the effects of the fuselage, effects of one rotor on the other, etc. Flow field velocities were acquired in a vertical plane at four aircraft azimuths of a small-scale tandem rotor system using the particle image velocimetry (PIV) technique for radial distances up to 4 times the rotor diameter. Results were compared against full-scale CH-47D measurements. Excellent correlation was found between the small- and full-scale mean flow fields (after appropriate normalization using rotor and wall jet parameters). Following the scalability analysis, the effect of rotor height on the outwash was also studied. Close to the aircraft, an increase in rotor height above ground decreased the outwash velocity at all aircraft azimuths. However, farther away, the longitudinal and lateral axes of the aircraft showed increasing and decreasing outwash velocities, respectively, with increasing rotor height. Measurements also indicated the presence of large-scale (of the size of the rotor height) shear-layer vortical structures along the ground that could be the source of low-frequency (approximately 1 Hz) flow variation observed in the full-scale measurements. Flow visualization studies and PIV measurements were also made on jets of different sizes to complement the observations made on rotors wherever possible. Baseline rotor measurements were made out-of-ground effect to understand the nature of inflow distribution for realistic rotor configurations and their modified characteristics in the presence of ground. Lastly, a feasibility study on applying high-fidelity CFD simulations for outwash study was conducted using Helios to model an isolated rotor configuration IGE at full scale Reynolds number. The results were encouraging and demonstrated the practical challenges associated with predicting rotor outwash.

Description: The flowfield and performance characteristics of highly-twisted blades were analyzed at various thrust conditions to improve the fundamental understanding relating the wake effects on rotor performance. Similar measurements made using untwisted blades served as the baseline case. Twisted blades are known to give better hover performance than untwisted blades at high thrust coefficients typical of those found in full-scale rotors. However, the present experiments were conducted at sufficiently low thrust (beginning from zero thrust), where the untwisted blades showed identical, if not better, performance when compared with the highly-twisted blades. The flowfield measurements showed some key wake differences between the two rotors, as well. These observations when combined with simple blade element momentum theory (also called annular disk momentum theory) helped further the understanding of rotor performance characteristics.