Publication Date:
2004-11-16
Description:
We have previously shown that in patients with MMM the number of circulating hematopoietic and endothelial progenitor cells is increased compared to other Ph-negative myeloproliferative disorders (Ph-CMPD). The aim of this study was to investigate the relevance of cytokines involved in the mobilization of progenitor cells from bone marrow to peripheral blood. We studied 120 patients with MMM, 22 patients with other Ph-CMPDs, and 30 healthy subjects. We evaluated by ELISA the plasma levels of stem cell factor (SCF), vascular endothelial growth factor (VEGF), and stromal derived factor-1alpha (SDF-1a). Moreover, the percentage of circulating CD34+ cells co-expressing the cytokine receptors CD117, VEGFR-2, or CXCR4 (for SCF, VEGF and SDF-1a, respectively) were assayed by flow cytometry. In all patients and controls, the percentage of circulating hematopoietic CD45+CD34+ cells and CD34+CD133+VEGFR-2+ endothelial progenitor cells were evaluated. SCF (median 872 pg/ml, range 281–2000), VEGF (median 38.9 pg/ml, range
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
Permalink