Publication Date:
2004-11-16
Description:
Objective: quantitative evaluation of viruria (DNA-uria) and hematuria in patients with SCT and analyze both relationship clinically. Methods and Materials: two PCR assays for detecting viruses in clinical specimens are established: quantifying virus load by real-time PCR and distinguishing BKV and JCV by seminested PCR. Intensity of hematuria, that is the dominant sign of hemorrhagic cystitis, was assessed by urinestick and classified into 6 grades: negative (−), trace or 1–10 (+), 11–25 (++), 26–80 (+++), 81–200 (++++) erythrocytes in 1 milliliter urine, and macrohematuria (+++++). 531 urine specimens were collected from 41 patients with SCT. Data were analyzed through SPSS software package. Results: Prevalence rate of polyoma viruria and hematuria following SCT were 80% and 73% in this study cohort and 45% and 20 % in urine specimens. No viral genomes were detected in DNA extracted from blood cells and plasma. BK, BK integrating with JC, JC and non-identified virus-variants, respectively, were shed in 15, 11, 3, 4 patients in urine and were detected in 99, 21, 32, 11 urine samples. Data of 25 patients with specimens collected before SCT showed that only 5 cases sheded polyoma virus in urine, comparing with 21 cases with polyoma viruria post-SCT, demonstrates significant difference (P= ++ and in 17% urine specimens with negative and only trace red blood cells, while hematuria with amount of erythrocytes 〉=++ presented in 21% BK-viruria, nevertheless in 9% urine specimens with negative or non-amplifying virus-variants, 7% JC-viruria and 6% BK integrating with JC-viruria (P=0.016). Time conformability was observed in onset of most viruria and hematuria within 2nd-3rd weeks post SCT. Conclusions: the course of SCT exerts explicitly impact on the reactivation of polyomavirus in urinary tract and BK-virus attribute likely to the onset of severer hematuria. Co-factors and pathogenesis are needed to continue exploring.
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
Permalink