Publication Date:
1991-06-14
Description:
In the interleukin-2 (IL-2) system, intracellular signal transduction is triggered by the beta chain of the IL-2 receptor (IL-2R beta); however, the responsible signaling mechanism remains unidentified. Evidence for the formation of a stable complex of IL-2R beta and the lymphocyte-specific protein tyrosine kinase p56lck is presented. Specific association sites were identified in the tyrosine kinase catalytic domain of p56lck and in the cytoplasmic domain of IL-2R beta. As a result of interaction, IL-2R beta became phosphorylated in vitro by p56lck. Treatment of T lymphocytes with IL-2 promotes p56lck kinase activity. These data suggest the participation of p56lck as a critical signaling molecule downstream of IL-2R via a novel interaction.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hatakeyama, M -- Kono, T -- Kobayashi, N -- Kawahara, A -- Levin, S D -- Perlmutter, R M -- Taniguchi, T -- New York, N.Y. -- Science. 1991 Jun 14;252(5012):1523-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute for Molecular and Cellular Biology, Osaka University, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2047859" target="_blank"〉PubMed〈/a〉
Keywords:
Adult
;
Animals
;
Antigens, CD/immunology
;
Base Sequence
;
Binding Sites
;
Cell Division/drug effects
;
Cell Line
;
Humans
;
Interleukin-2/pharmacology
;
Killer Cells, Natural/cytology/drug effects/immunology
;
Lymphocyte Activation
;
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
;
Lymphocytes/drug effects/*immunology
;
Macromolecular Substances
;
Molecular Sequence Data
;
Molecular Weight
;
Oligonucleotide Probes
;
Protein-Tyrosine Kinases/genetics/isolation & purification/*metabolism
;
Receptors, Interleukin-2/genetics/isolation & purification/*physiology
;
*Signal Transduction
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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