Publication Date:
1997-02-28
Description:
A complementary DNA clone has been isolated that encodes a coxsackievirus and adenovirus receptor (CAR). When transfected with CAR complementary DNA, nonpermissive hamster cells became susceptible to coxsackie B virus attachment and infection. Furthermore, consistent with previous studies demonstrating that adenovirus infection depends on attachment of a viral fiber to the target cell, CAR-transfected hamster cells bound adenovirus in a fiber-dependent fashion and showed a 100-fold increase in susceptibility to virus-mediated gene transfer. Identification of CAR as a receptor for these two unrelated and structurally distinct viral pathogens is important for understanding viral pathogenesis and has implications for therapeutic gene delivery with adenovirus vectors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bergelson, J M -- Cunningham, J A -- Droguett, G -- Kurt-Jones, E A -- Krithivas, A -- Hong, J S -- Horwitz, M S -- Crowell, R L -- Finberg, R W -- AI31628/AI/NIAID NIH HHS/ -- AI35667/AI/NIAID NIH HHS/ -- CA69703/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1997 Feb 28;275(5304):1320-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Infectious Diseases, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9036860" target="_blank"〉PubMed〈/a〉
Keywords:
Adenoviruses, Human/genetics/*metabolism/physiology
;
Amino Acid Sequence
;
Animals
;
CHO Cells
;
Coxsackie and Adenovirus Receptor-Like Membrane Protein
;
Cricetinae
;
Cytopathogenic Effect, Viral
;
Enterovirus B, Human/*metabolism/physiology
;
Gene Transfer Techniques
;
Genetic Vectors
;
HeLa Cells
;
Humans
;
Molecular Sequence Data
;
Receptors, Virus/chemistry/genetics/*isolation & purification/metabolism
;
Sequence Alignment
;
Transfection
;
Virus Replication
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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