ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    facet.materialart.
    Unknown
    Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression (2007)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2007-12-15
    Description: RNA polymerase II (Pol II) transcribes genes that encode proteins and noncoding small nuclear RNAs (snRNAs). The carboxyl-terminal repeat domain (CTD) of the largest subunit of mammalian RNA Pol II, comprising tandem repeats of the heptapeptide consensus Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7, is required for expression of both gene types. We show that mutation of serine-7 to alanine causes a specific defect in snRNA gene expression. We also present evidence that phosphorylation of serine-7 facilitates interaction with the snRNA gene-specific Integrator complex. These findings assign a biological function to this amino acid and highlight a gene type-specific requirement for a residue within the CTD heptapeptide, supporting the existence of a CTD code.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2263945/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2263945/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Egloff, Sylvain -- O'Reilly, Dawn -- Chapman, Rob D -- Taylor, Alice -- Tanzhaus, Katrin -- Pitts, Laura -- Eick, Dirk -- Murphy, Shona -- 072107/Wellcome Trust/United Kingdom -- 081312/Wellcome Trust/United Kingdom -- G0400653/Medical Research Council/United Kingdom -- G0400653(71330)/Medical Research Council/United Kingdom -- G9826944/Medical Research Council/United Kingdom -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 2007 Dec 14;318(5857):1777-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18079403" target="_blank"〉PubMed〈/a〉
    Keywords: Alanine ; Amino Acid Sequence ; Cell Line ; Consensus Sequence ; *Gene Expression Regulation ; Heterogeneous-Nuclear Ribonucleoproteins/genetics ; Humans ; Mutation ; Oligopeptides/chemistry/metabolism ; Phosphorylation ; Protein Structure, Tertiary ; Protein Subunits/genetics/metabolism ; RNA Polymerase II/chemistry/genetics/*metabolism ; RNA Processing, Post-Transcriptional ; RNA, Messenger/genetics/metabolism ; RNA, Small Nuclear/*genetics ; Serine/*metabolism ; Templates, Genetic ; *Transcription, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 2
    facet.materialart.
    Unknown
    Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7 (2007)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2007-12-15
    Description: RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Differential phosphorylation of serine-2 and serine-5 at the 5' and 3' regions of genes appears to coordinate the localization of transcription and RNA processing factors to the elongating polymerase complex. Using monoclonal antibodies, we reveal serine-7 phosphorylation on transcribed genes. This position does not appear to be phosphorylated in CTDs of less than 20 consensus repeats. The position of repeats where serine-7 is substituted influenced the appearance of distinct phosphorylated forms, suggesting functional differences between CTD regions. Our results indicate that restriction of serine-7 epitopes to the Linker-proximal region limits CTD phosphorylation patterns and is a requirement for optimal gene expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chapman, Rob D -- Heidemann, Martin -- Albert, Thomas K -- Mailhammer, Reinhard -- Flatley, Andrew -- Meisterernst, Michael -- Kremmer, Elisabeth -- Eick, Dirk -- New York, N.Y. -- Science. 2007 Dec 14;318(5857):1780-2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18079404" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Antibodies, Monoclonal ; Cell Line, Tumor ; Chromatin Immunoprecipitation ; Epitopes ; *Gene Expression Regulation ; Genes, T-Cell Receptor beta ; Humans ; Molecular Sequence Data ; Oligopeptides/chemistry/metabolism ; Phosphorylation ; Protein Subunits/chemistry/immunology/metabolism ; RNA Polymerase II/chemistry/genetics/immunology/*metabolism ; Serine/*metabolism ; *Transcription, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    PAPER CURRENT
  • 3
    Electronic Resource
    Electronic Resource
    Benzothiophene Chemistry. VI. A Peroxide Effect in the Addition of Thiophenols to Benzothiophene 1-Dioxide (1954)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 76 (1954), S. 3637-3639 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja01643a009
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Calibration of OSO-7 Fexv 284Å spectroheliograms in solar-geophysical data bulletin (1974)
    Springer
    Solar physics 38 (1974), S. 415-417 
    Details
    ISSN: 1573-093X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00155078
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    OSO-7 results on coronal emission near 304 Å (1975)
    Springer
    Solar physics 43 (1975), S. 337-350 
    Details
    ISSN: 1573-093X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The spectral composition and spatial distribution of equatorial coronal emission near 304 Å is examined. Spectral scans indicate that the predominant line is from Si xi. Comparisons of observations with calculations of intensity changes with altitude indicate that collisional excitation is important near the Sun but that photoexcitation becomes dominant beyond about 1.3 R ⊙ from the solar center. Observed and calculated intensities are in approximate agreement for abundances and electron densities that are within the range of observed values.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00152358
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    On coronal Fe abundances and temperatures from XUV emission lines (1977)
    Springer
    Solar physics 53 (1977), S. 435-444 
    Details
    ISSN: 1573-093X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Data obtained by the OSO-7 spectroheliograph on strong XUV lines of five, different Fe ions from the outer equatorial corona are presented. Interpretation of the data with a spherically symmetric model atmosphere gives average ion abundances for lines of sight at 0.3R ⊙ from the limb. Fe xvi is usually more abundant than Fe xv, xiv, xii and ix, but there are times when Fe xii is more abundant than the other ions. The deviation of measured relative abundances of Fe xii, xiv, and xvi from predictions of ionization equilibrium at one temperature seems to indicate that there are appreciable temperature variations along lines of sight. Element abundances are very uncertain since they appear to depend so heavily on likely but unknown density irregularities along lines of sight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00160286
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Abundance of Fe relative to H at 1.5 solar radii (1975)
    Springer
    Solar physics 42 (1975), S. 487-496 
    Details
    ISSN: 1573-093X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The abundance of Fe relative to H is obtained by using resonantly scattered intensities of λ 284 of Fe XV that were measured with OSO-7 and resonantly scattered intensities of Lα of H I that were obtained by Gabriel (1971). Because of possible differences in electron densities along lines of sight for these non-simultaneous measurements and in relative calibrations, results are rather uncertain but still indicate that the average Fe abundance relative to H in the corona appears to be at least as large as a recent photospheric abundance. Some limitations in using this method for obtaining abundances are examined for future experiments with simultaneous measurements and well calibrated detectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00149928
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Spatial distribution of soft X-ray and EUV emission associated with a chromospheric flare of importance 1B on August 2, 1972 (1974)
    Springer
    Solar physics 34 (1974), S. 349-375 
    Details
    ISSN: 1573-093X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Soft-X-ray and extreme ultraviolet spectroheliographs carried by the OSO-7 (Orbiting Solar Observatory) have been used to record the development of XUV emission associated with a flare of importance 1b on August 2, 1972. Spatial resolution was 20″ and spectral resolution was adequate to select emission lines originating within well-defined ranges of electron temperature between 5 × 104 and 30 × 106K. The data show that heating only the pre-existing coronal material adjacent to the flare site cannot account for the soft X-ray emission measure observed during the event. The flare emission originating at T e ⩽ 2.3 × 106K exhibits an impulsive component coincident with an impulsive microwave event. This radiation appears to coincide spatially with Hα radiation emitted at that time and is centered on the neutral line separating magnetic fields of opposite polarity. One soft X-ray-emitting feature, estimated to have an initial electron temperature of 2–10 × 106K, forms during the impulsive phase immediately over the Hα flare. A second, arch-like feature observed at wavelengths near 1.9 Å and estimated to have T e≅30 × 106K is located approximately 35000 km above the Hα event. Both regions have lengths of about 27000 km but transverse dimensions small compared to the spatial resolution of the spectroheliograph, i.e., less than 14000 km. The region with highest electron temperature exhibits the greatest stability in position. This region cools to approximately 10 × 106K in 6 to 12 min which is compatible with cooling by conduction to the chromosphere. The best association with a dark surge is found in an emission line of Fe xiv.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00153673
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Coronal temperatures and temperature gradients from OSO-7 spectroheliograms (1976)
    Springer
    Solar physics 47 (1976), S. 611-626 
    Details
    ISSN: 1573-093X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Temperatures and temperature gradients for the outer corona are obtained from brightness gradients of EUV lines that were measured with the spectroheliograph on OSO-7. Brightness gradients show considerable deviations from isothermal model calculations that include collisional excitation and photoexcitation. A negative temperature gradient that gives both positive and negative ion abundance gradients appears to be able to account for the discrepancy. For λ284 of Fe xv, perhaps the strongest line from the outer corona, measurements during 1972 appear to be consistent with (i) a temperature near 2.3×106K near the equator at ϱ = 1.3±0.1 solar radii from the solar center; (ii) (ϱ/T) dT/dϱ values near -0.7 that extend from as low as ϱ = 1.2 to about ϱ = 1.8. Temperatures from strong lines of Fe xiv and Fe xvi indicate that variations of about ±0.2×106K exist along lines of sight where emission is appreciable. There appears to be some agreement between these results and temperature measurements from ion abundances in the solar wind and Doppler width of λ5303.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00154770
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Deuterium Isotope Effects during HMX Combustion: Chemical kinetic burn rate control mechanism verifiedsee Ref. 1. (1989)
    Weinheim : Wiley-Blackwell
    Propellants, Explosives, Pyrotechnics 14 (1989), S. 93-102 
    Details
    ISSN: 0721-3115
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The appearance of a significant deuterium isotope effect during the combustion of the solid HMX compound verifies that the chemical reaction kinetics is a major contributor in determining the experimentally observed or global burn rate. Burn rate comparison of HMX and its deuterium labeled HMX-d8 analogue reveals a primary kinetic deuterium isotope effect (1° KDIE) at 500 psig (3.55 MPa) and l000 psig (6.99 MPa) pressures and selectively identifies covalent carbon-hydrogen bond rupture as the mechanistic step which ultimately controls the HMX bum rate under the static combustion conditions of this experiment. The 1° KDIE value further suggests the rate-limiting C—H bond rupture occurs during the solid state HMX decomposition/deflagration portion of the overall combustion event and is supported by other independently published studies. A possible anomalous KDIE result at 1500 psig (10.4 MPa) is addressed. This condensed phase KDIE approach illustrates a direct link between lower temperature/pressure thermal decomposition and deflagration processes and their potential applicability to the combustion regime. Most importantly, a new general method is demonstrated for mechanistic combustion investigations which selectively permits an in-situ identification of the compound's burn rate-controlling step.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prep.19890140303
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...