Publication Date:
1993-01-08
Description:
Soon after methicillin was introduced into clinical practice in the early 1960s, resistant strains of Staphylococcus aureus (MRSA) appeared, bearing a newly acquired resistance gene, mecA, that encodes a penicillin binding protein, PBP2a. MRSA have spread throughout the world, and an investigation of the clonality of 472 isolates by DNA hybridization was performed. All 472 isolates could be divided into six temporally ordered mecA hybridization patterns, and three of these were subdivided by the chromomosomal transposon Tn554. Each Tn554 pattern occurred in association with one and only one mecA pattern, suggesting that mecA divergence preceded the acquisition of Tn554 in all cases and therefore that mecA may have been acquired just once by S. aureus.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kreiswirth, B -- Kornblum, J -- Arbeit, R D -- Eisner, W -- Maslow, J N -- McGeer, A -- Low, D E -- Novick, R P -- AI22159/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 1993 Jan 8;259(5092):227-30.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Bureau of Laboratories, New York City Department of Health, NY 10016.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8093647" target="_blank"〉PubMed〈/a〉
Keywords:
*Bacterial Proteins
;
Biological Evolution
;
Carrier Proteins/genetics
;
DNA Transposable Elements
;
DNA, Bacterial/analysis/genetics
;
Deoxyribonucleases, Type II Site-Specific
;
*Hexosyltransferases
;
Methicillin Resistance/*genetics
;
Muramoylpentapeptide Carboxypeptidase/genetics
;
Nucleic Acid Hybridization
;
Penicillin-Binding Proteins
;
*Peptidyl Transferases
;
Polymorphism, Restriction Fragment Length
;
Staphylococcus aureus/drug effects/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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