ISSN:
1432-1955
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Cytochemical technique associated with transmission electron microscopy were used for the localization inTritrichomonas foetus of enzymes used as markers of different cell structures. Reaction product indicating the presence of Mg2+-adenosine triphosphatase (Mg2+-ATPase) and 5′-nucleotidase was observed in the plasma membrane. Glucose-6-phosphatase was seen in association with the endoplasmic reticulum, revealing its organization as parallel cisternae. Thiaminopyrophosphatase was located in thecis-most region of the Golgi complex. Acid phosphatase was found within lysosomes as well as in several cisternae of the Golgi complex, in contrast to previous observations in mammalian cells. These observations provide support for the use of enzyme markers in future studies on cell fractionation ofT. foetus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00931013
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