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  • 1
    Electronic Resource
    Electronic Resource
    Jack and the beanstalk: Legume biotechnology, past, present and future Genetic engineering and in vitro culture of crop legumes - by Paul Christou, Technomic Publishing Company, 1992. SFr 143.00 (315 pages) ISBN 0 87762 946 3
    Amsterdam : Elsevier
    Trends in Biotechnology 11 (1993), S. 519-520 
    Details
    ISSN: 0167-7799
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0167-7799(93)90033-6
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  • 2
    Electronic Resource
    Electronic Resource
    Production of stably transformed cassava plants via particle bombardment (2000)
    Springer
    Plant cell reports 19 (2000), S. 939-945 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cassava ; (Manihot esculenta Crantz) ; Transformation ; Biolistics ; Organogenesis ; Rooting test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A novel protocol, based on biolistics and regeneration via organogenesis, was developed for genetic transformation of cassava (Manihot esculenta Crantz). The in vitro performance of cassava cultivars CMC40, MPer183 and MCol22 was evaluated, and the regeneration protocol was modified to improve shoot production from explants for transformation experiments. Somatic cotyledons were used as a target tissue in the transformation experiments using the Particle Inflow Gun and a plasmid containing the uidA gene in transient assays. The effect of different parameters for particle bombardment efficiency, including the amount of DNA used, the flying distance of the projectiles and the pre- and post-plasmolysis time of the target tissue, was evaluated and the conditions were partially optimised. Stably transformed cassava plants of cvs. MCol22 and TMS60444 were produced using the partially optimised conditions and two different vector constructs carrying the hpt gene as the selectable marker. The selection protocol was optimised further, and a rooting test was developed for screening the regenerants for antibiotic resistance to reduce the number of escapes obtained after primary selection. The production of stably transformed cassava lines and the expression of the transgenes was verified by Southern blot analysis and RT-PCR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000224
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  • 3
    Electronic Resource
    Electronic Resource
    PIG-mediated cassava transformation using positive and negative selection (2000)
    Springer
    Plant cell reports 19 (2000), S. 1041-1048 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cassava (Manihot esculenta Crantz) ; Transformation ; Biolistics ; Positive selection ; Negative selection ; Abbreviations2,4-D 2,4-Dichlorophenoxyacetic acid ; BA 6-Benzylaminopurine ; CBM Basic medium ; CEM Elongation medium ; CIM Embryo induction medium ; CMM Maturation medium ; COM Organogenesis medium ; CTM Transformation medium ; GD Gresshoff and Doy medium ; IBA Indole-3-butyric acid ; FEC Friable embryogenic callus ; NAAα-Naphthalene acetic acid ; MSM Solid mannose selection medium ; MSN FEC Formation and embryo conversion medium ; SCV Settled cell volume ; SH Schenk and Hildebrandt medium ; SHM Liquid mannose selection medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In order to develop new selection systems for production of transgenic cassava (Manihot esculenta Crantz), two different selection regimes were assessed for their efficiency on regeneration of transgenic cassava plants: positive selection using mannose and negative selection using hygromycin. Explants from somatic cotyledons and embryogenic suspensions were used as target tissues in the transformation experiments and bombarded using the particle inflow gun. Different culture and selection strategies were assessed to optimise the selection protocols. For the first time transgenic plants could be obtained using positive, and in the case of embryogenic suspensions, hygromycin-based negative selection. The stably transformed nature of the regenerated cassava plant lines and the expression of the transgenes were verified with PCR, RT-PCR, Southern and northern analyses. A rooting test for transgenic plants on a medium supplemented with mannose was developed to further improve the efficacy of the positive selection system. Our results demonstrate that it is possible to obtain transgenic cassava plants using non-antibiotic positive selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000245
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  • 4
    Electronic Resource
    Electronic Resource
    Regeneration of cassava plants via shoot organogenesis (1998)
    Springer
    Plant cell reports 17 (1998), S. 410-414 
    Details
    ISSN: 1432-203X
    Keywords: Key words Cassava ; Manihot esculenta Crantz ; Shoot regeneration ; Organogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel regeneration system based on direct shoot organogenesis is described for cassava. Plants could be regenerated at high frequency by inducing shoot primordia on explants derived from cotyledons of cassava somatic embryos. After a passage on elongation medium, the regenerated shoots were easily rooted in hormone-free medium and could be successfully transplanted to soil. Using the shoot-organogenesis-based regeneration method, up to eight transplantable plantlets per explant could be regenerated. The system was optimised first for one cassava cultivar, and then its transferability to three other cultivars was demonstrated. This method widens the scope of in vitro regeneration modes of cassava, and is also compatible with Agrobacterium-mediated transformation. To develop an efficient system for production of somatic embryos for regeneration experiments, conditions for inducing primary and cycling somatic embryos were also studied, and highly efficient plant regeneration via germination of somatic embryos was achieved using maltose instead of sucrose in the culture medium, and combining paclobutrazol with 2,4-dichlorophenoxyacetic acid in the embryo induction medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050416
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  • 5
    Electronic Resource
    Electronic Resource
    Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens — mediated gene transfer (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 246-252 
    Details
    ISSN: 1432-2242
    Keywords: Transformation ; Pea ; Pisum sativum ; Agrobacterium tumefaciens ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A transformation system that allows regeneration of transgenic pea plants from calli selected for antibiotic resistance was developed. Explants from axenic shoot cultures and seedling epicotyls were cocultivated with nononcogenic Agrobacterium tumefaciens strains, and transformed callus could be selected on callus-inducing media containing either 15 mg/l hygromycin or 75 mg/l kanamycin. After several passages on regeneration medium, shoot organogenesis could be reproducibly induced on hygromycin-resistant calli, but not on the calli selected for kanamycin resistance. Regenerated shoots could subsequently be rooted and transferred into the greenhouse. In addition, the effects of different callus-inducing and growth media on organogenesis were investigated. The transformation of the calli and regenerated plants was confirmed by DNA analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224394
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  • 6
    Electronic Resource
    Electronic Resource
    Inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 443-450 
    Details
    ISSN: 1432-2242
    Keywords: Transformation ; Pisum sativum ; Agrobacterium tumefaciens ; Regeneration ; Transgenic plants ; Progeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An analysis of the progeny of primary transgenic pea plants in terms of transmission of the transferred DNA, fertility and morphology is presented. A transformation system developed for pea that allows the regeneration of fertile transgenic pea plants from calli selected for antibiotic resistance was used. Expiants from axenic shoot cultures were co-cultivated with a nononcogenic Agrobacterium tumefaciens strain carrying a gene encoding hygromycin phosphotransferase as selectable marker, and transformed callus could be selected on callus-inducing media containing 15 mg/l hygromycin. After several passages on regeneration medium, shoot organogenesis could be reproducibly induced on the hygromycin resistant calli, and the regenerated shoots could subsequently be rooted and transferred to the greenhouse, where they proceeded to flower and set seed. The transmission of the introduced gene into the progeny of the regenerated transgenic plants was studied over two generations, and stable transmission was shown to take place. The transgenic nature of the calli and regenerated plants and their progeny was confirmed by DNA and RNA analysis. The DNA and ploidy levels of the progeny plants and primary regenerants were studied by chromosome analysis, and the offspring of the primary transformants were evaluated morphologically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229505
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