Publication Date:
2018-04-27
Description:
RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors RNA-guided transcriptional silencing in vivo with 〈i〉S. aureus〈/i〉 CRISPR-Cas9 repressors, Published online: 26 April 2018; doi:10.1038/s41467-018-04048-4 Repression of gene transcription using CRISPR-Cas9 has been achieved in vitro but not for delivery into adult animal models. Here, the authors use AAV8 to deliver the transcriptional repressor dSaCas9KRAB to the cholesterol regulator Pcsk9, and show repression up to 24 weeks and reduced cholesterol levels in mice.
Electronic ISSN:
2041-1723
Topics:
Biology
,
Chemistry and Pharmacology
,
Natural Sciences in General
,
Physics
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