ISSN:
1432-1955
Keywords:
AbbreviationsMTT 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide
;
MDR Multiple-drug resistance
;
EGTA Ethylene glycol bis(2-aminoethyl ether)-N,N,N′N′-tetraacetic acid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract An arsenite-resistant strain of Leishmania donovani was generated in vitro by sequential exposure to higher concentrations of sodium m-arsenite. The resistant strain displayed a low level of cross-resistance to structurally unrelated drugs such as doxorubicin and pentamidine. This cross-resistance was sensitive to the calcium-channel blocker verapamil. The membrane-associated P-type adenosine triphosphatase (ATPase) activity detected in crude membrane fractions of the resistant strain was 3-fold that found in the wild-type parasites. The enhanced ATPase activity was unaffected by the presence of verapamil in the reaction mixture. However, when cells were grown in the presence of verapamil the membrane-associated ATPase activity of the resistant strain showed significant inhibition.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00008548
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