ISSN:
1573-5028
Keywords:
ACC oxidase
;
Cucumis melo
;
ethylene
;
gene expression
;
wound
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The enzyme ACC oxidase catalyses the last step of ethylene biosynthesis in plants. Expression of the melon ACC oxidase gene, CM-ACO1, is rapidly induced (within 10 min) by ethylene treatment or upon wounding in leaves. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), inhibited the accumulation of ethylene-induced CM-ACO1 mRNA transcripts, while wound-induced expression of the gene was not affected. The 5′-untranslated region of the CM-ACO1 gene was fused to the β-glucuronidase (GUS) reporter gene and the corresponding transgenic tobacco plants were analysed. Two separate regions of the CM-ACO1 promoter activated GUS expression in response to ethylene treatment and wounding. These results suggest that induction of CM-ACO1 gene expression occurs via two separate signal transduction pathways in response to wounding and ethylene treatment.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005902226054
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