ISSN:
1573-4846
Keywords:
lactate dehydrogenase
;
sol-gel
;
biosensor
;
lactic acid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Physical adsorption and physical entrapment techniques have been utilized for the immobilization of lactate dehydrogenase (LDH) on tetraethylorthosilicate (TEOS) derived sol-gel films. The enzyme (LDH) activity has been assayed as a function of time, temperature, pH and pyruvate concentration. The results of photometric measurements used for monitoring the reaction yield a response time of about 1 min, linearity over a concentration range of 0–1.5 × 10-3 M and detection limit of 5 × 10-5 M. The TEOS sol-gel films containing LDH have been found to be stable for about 30 days at temperatures 4 to 10°C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018329518938
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