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  • 1
    Publication Date: 1998-07-01
    Print ISSN: 0011-183X
    Electronic ISSN: 1435-0653
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Published by Wiley
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The possibility of using random amplified polymorphic DNA (RAPD) markers previously mapped in the common bean PC50/XANI59 population to select for resistance to common bacterial blight (CBB) in different populations was examined. Two out of 02 selected RAPD markers were polymorphic in HR56 and W0633d, the parental lines used in this experiment. Cosegregation analysis of the two polymorphic markers and disease reaction in a recombinant inbred (RI) population derived from HR67/W1744d confirmed that one of the two RAPD markers, BC420900, was significantly associated with a major quantitative trait locus-conditioning resistance to CBB in HR67. This locus accounted for approximately 51) of the phenotypic variation. The RAPD marker was transformed into a sequence characterized amplified region (SCAR) marker and used for selection in a different population derived from ‘Envoy’/HR67. Prediction for resistance to CBB with the BC420.990 SCAR marker was 94.2% accurate in this population. A comparison between marker-assisted selection (MAS) and conventional greenhouse screening showed that the cost of MAS is about one-third less than that of the greenhouse test.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-203X
    Keywords: Key words Chitinase ; Tomato ; Lycopersicon chilense ; Verticillium dahliae ; Disease tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An acidic endochitinase gene (pcht28) isolated from Lycopersicon chilense was introduced into tomato (L. esculentum) through Agrobacterium-mediated transformation, using the CAMV 35S promoter. Transgenic plants demonstrated a high level of constitutive expression of pcht28 and chitinase enzyme activity. Kanamycin-resistant R1 plants (resulting from self-pollination of transgenic plants) as well as R2 plants were evaluated for their tolerance to Verticillium dahliae (race 1 and 2 for R1 plants and race 2 for R2 plants) in the greenhouse. They demonstrated a significantly (P〈0.05) higher level of tolerance to the fungi compared to the nontransgenic plants, as measured by foliar disease symptoms, vascular discoloration, and vascular discoloration index. The transgenic plants produced in this study represent a source of genetic resistance to Verticillium dahliae.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 8 (1989), S. 21-24 
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Longitudinal sections containing cortical cells taken from stem internodes of a hybrid betweenLycopersicon esculentum andSolanum lycopersicoides were used as tissue sources for enzymatic protoplast isolation. Greenhouse and growth room-grown plants 4–8 weeks after rooting could be used as sources of donor tissue. Protoplasts from these tissues divided within 2–4 days of culture and numerous microcalli formed within 30 days. The shoot regeneration frequency of protoplast-derived calli was in the order of 60%. More than 100 regenerated plants which appear phenotypically normal have been established in soil.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 187-192 
    ISSN: 1432-2242
    Keywords: Lycopersicon peruvianum ; Lycopersicon esculentum ; Interspecific hybridization ; Embryo callus culture ; Solanum lycopersicoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using a modified embryo callus culture technique, hybrids between Lycopersicon esculentum and L. peruvianum were developed and their usefulness as bridge lines for facilitating interspecific gene transfer was evaluated. Four of these lines showed a high level of sexual compatibility with several other L. peruvianum var. typicum accessions, as well as with accessions of L. peruvianum var. humifusum and L. peruvianum var. glandulosum and L. esculentum. These bridge line x L. peruvianum hybrids could be crossed with L. esculentum to introgress genes from L. peruvianum into L. esculentum.
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  • 6
    ISSN: 1435-0653
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Glycine max (L.) Merr.] cultivars. Tawny-pubescent yellow-hilum genotypes are sometimes described with buff, light brown, or very light brown hila in Canada. This study was conducted to determine whether the yellow hila of tawny-pubescent lines discolor more than grey-pubescent lines, and whether therefore these yellow hila may actually be classified as brown rather than yellow. Seed of tawny- and grey-pubescent yellow-hila isolines in two genetic backgrounds grown in three or four seasons were scored (1, light, to 4, dark) for hilum color by soybean breeders and also classified by the Variety Verification Unit, Canadian Food Inspection Agency. Hilum color was scored darker and non-yellow (2.9 score) for tawny-pubescent lines compared with yellow (1.4 score) for grey-pubescent lines. The effects of year and genetic background were also significant. Generally, non-yellow and yellow hila were found in tawny-pubescent lines while only yellow hila were found in grey-pubescent lines. We propose recognition of a new hilum color, imperfect yellow, conditioned by II, rr, and TT. The hilum color imperfect yellow has been approved by the Soybean Genetics Committee. The color of imperfect yellow hila ranges from yellow to brown depending on the genetic background of the line and environmental conditions.
    Type of Medium: Electronic Resource
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