ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hits 1 - 3 | 3 hits

Sorting

Electronic Resource

Morphometric analysis of the autophagic and crinophagic lysosomal systems in mammotropes throughout the estrous cycle of the rat (1981)

Poole, Max C. ; Mahesh, Virendra B. ; Costoff, Allen

Springer

Cell & tissue research 220 (1981), S. 131-137

add to mindlist on the mindlist

Details

ISSN: 1432-0878

Keywords: Rat ; Adenohypophysis ; Mammotropes ; Autophagic and crinophagic lysosomes ; Morphometry

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary The crinophagic and autophagic lysosomal systems were studied in mammotropes (prolactin secreting cells) of the adenohypophysis throughout the estrous cycle of the rat. By means of morphometric analysis, it was found that the volume of secondary autophagic lysosomes was usually greater than that of the crinophagic type. Although the volumes of both secondary autophagic and crinophagic lysosomes were minimal throughout proestrus and diestrus 2, the autophagic lysosomal volume per mammotrope was elevated during the estrous period. The volume of secondary crinophagic lysosomes per mammotrope increased during late estrus and remained elevated throughout early diestrus 1. Furthermore, there was an inverse relationship between the volume of mature secretory granules per cell and of the crinophagic system. These data suggest a role for lysosomes in the regulation of synthesis and secretion of prolactin by the adenohypophysis of the rat.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00209972

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

An image processing/stereological analysis system for transmission electron microscopy (1992)

Poole, Max C.

New York, NY [u.a.] : Wiley-Blackwell

Microscopy Research and Technique 21 (1992), S. 283-291

add to mindlist on the mindlist

Details

ISSN: 1059-910X

Keywords: Computer imaging ; Cell ultrastructure ; Morphometry ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Natural Sciences in General

Notes: This study examines the feasibility of combining computer image digitization, image enhancement, and point counting stereological techniques to quantify video images from transmission electron microscopes (TEM). The essential hardware consists of an IBM PC/AT, a Matrox imaging board, a digitizing tablet, a high resolution black and white monitor, and a portable mass storage device. In addition a video camera must be mounted to the TEM. The software is written in three modules which have numerous routines for image acquisition, enhancement, and quantification. Quantification is achieved by selecting an electronic lattice and superimposing it on the cell image. A cursor is moved on the lattice (via the digitizing tablet) and the points are entered into a spreadsheet. One of the major limitations of the system was the reduced resolution inherent in the current hardware. However, sampling experiments showed that one could compensate for the reduced resolution by increasing the magnification of the digitized images, and the stereological values from digitized images compared favorably to those from electron micrographs. Furthermore, the system proved advantageous by eliminating the usual darkroom work, and in enhancing low contrast tissue. In spite of several hardware limitations, the concept of quantifying computer digitized TEM images appears promising. © 1992 Wiley-Liss, Inc.

Additional Material: 5 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/jemt.1070210405

Permalink