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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 159 (1975), S. 523-529 
    ISSN: 1432-0878
    Keywords: Experimental calcinosis ; KMnO4treatment ; Intracellular crystal deposits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mineralized plaques, which develop at the site of repeated subcutaneous injections of 100 μg KMnO4/0.2 H2O in rats, were investigated by electron microscopy. The newly formed, delineated, white plaque tissue at the injection site consisted of numerous, mostly unaltered fibroblasts and collagen fibers, without participation of inflammatory cells. Some signs of cell injury were found in the center of the lesions. Numerous, irregularly distributed, small, mineralized foci were seen near the fibroblasts. These were formed by aggregation of small needle-like units (50 Å in diameter and 0.05–2.0 μm long). These needle-shaped units were found either in vesicular, cell derived structures, considered to be shed cell processes or cell fragments, or on collagen fibers. Intramitochondrial deposits of such needle-like units were seen frequently. Fusion of smaller mineralized foci to larger plaques occured and then needle-shaped units were seen at the periphery of the electron-dense lesions. Hypotheses concerning the mechanism of experimental cutaneous calcinosis (soft tissue mineralization) are discussed and related to the findings of this study. Probable intracellular crystal deposition and mineralization in cell-derived structures were shown for the first time in topical cutaneous calcinosis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 123 (1972), S. 496-507 
    ISSN: 1432-0878
    Keywords: Rat incisor ; Tooth eruption ; Ectopic enamel organ ; Electronmicroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Durch regelmäßig wiederholtes mechanisches Kürzen des rechten unteren Rattenschneidezahnes wurde eine beschleunigte Eruption hervorgerufen. Nach 15–17 Tagen trat eine Störung der Schmelzentwicklung in Form einer Produktion von kreideweißem statt normal pigmentiertem Schmelz und ein rotbräunliches Häutchen im Bereich des gingivalen Epithelverschlusses in Erscheinung. Dieses Häutchen wurde nach intravitaler Fixierung mit Glutaraldehyd abpräpariert und licht- und elektronenmikroskopisch untersucht. Das Häutchen besteht aus mehreren übereinander gelagerten Zellschichten: aus Ameloblasten, einem typischen Papillarorgan, einer Bindegewebsschicht und aus flachen Deckzellen. Die Ameloblasten im Bereich des Epithelverschlusses befinden sich im Stadium der frühen, die Ameloblasten des aus dem Epithelverschluß hervorwachsenden Häutchens dagegen im Stadium der späten Maturation. Die Ultrastruktur des gesamten Papillarorgans läßt auf eine intensive metabolische Funktion und auf Ionentransport schließen. Pigmentgranula, die bei normaler Eruption im Maturationsstadium sowohl im Papillarorgan als auch in den Ameloblasten vorkommen, wurden nur im Bereiche des Epithelverschlusses in vaskulären Elementen und in perivaskulären Bindegewebszellen gefunden. Die Oberfläche des Häutchens wird von einer schmalen Schicht flacher, organellenarmer Zellen gebildet. Das bei beschleunigter Eruption aus dem Epithelansatz hervorwachsende Häutchen ist ein ektopisches Schmelzorgan, dessen rotbräunliche Färbung von der reichen Gefäßversorgung herrührt.
    Notes: Summary An accelerated eruption of the right mandibular rat incisor was induced by its repeated, mechanical shortening. Fifteen to seventeen days after beginning of the experiment a disturbance of enamel development occured, manifested by production of white, opaque enamel instead of normally pigmented enamel and by appearance of a redbrownish membrane overlying the enamel. This membrane was intravitally fixed with glutaraldehyde and after dissection processed for histologic and electronmicroscopic investigation. The membrane consists of ameloblasts, a typical papillary organ, a connective tissue layer and of flat surface cells. The ameloblasts near the epithelial attachment are in early maturation state, the ameloblasts of the outgrowing membrane show signs of reduction, typical for late maturation. The papillary organ of the entire membrane showes ultrastructural equivalents of high metabolic activity as well as of fluid transports. Pigment granules, which in normal eruption occur in the maturation state in the papillary cells and in ameloblasts, are absent in these cells of the membrane. They were only seen in perivascular elements and in connective tissue cells near the epithelial attachment. On the oral surface the membrane is covered by a thin layer of flat, degenerated cells poor in organelles. These findings support the concept, that the membrane growing out of the epithelial attachment in accelerated eruption of the rat incisor, is an ectopic enamel organ. The red-brownish colour is not due to pigment accumulation but to its rich vascularization.
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  • 3
    Publication Date: 1975-06-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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  • 4
    Publication Date: 1972-12-01
    Print ISSN: 0302-766X
    Electronic ISSN: 1432-0878
    Topics: Biology , Medicine
    Published by Springer
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