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In vivo and in vitro effects of nickel and cadmium on the plasmalemma ATPase from rice (Oryza sativa L.) shoots and roots

Ros, R. ; Morales, A. ; Segura, J. ; [et al.]

Amsterdam : Elsevier

Plant Science 83 (1992), S. 1-6

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ISSN: 0168-9452

Keywords: ATPase ; cadmium ; nickel ; plasmalemma ; rice

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Biology

Type of Medium: Electronic Resource

URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(92)90055-Q

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Characterization of ATPase activities in rice root plasmalemma vesicles isolated by two-phase partitioning (1991)

Ros, R. ; Sanz, A. ; Segura, J. ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Physiologia plantarum 83 (1991), S. 0

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ISSN: 1399-3054

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Biology

Notes: Plasma membrane vesicles were isolated from the roots of 7-day-old rice plants (Oryza sativa L. cv. Bahía) by utilizing an aqueous polymer two-phase system with 6.2%:6.2% (w/w) Dextran T500 and polyethylene glycol 3350 (PEG) at pH 7.6. Plasmalemma vesicles of high purity were obtained as indicated by the vanadate-sensitive K+, Mg2+-ATPase activity that was 18 times higher in the upper (PEG-rich) phase than in the lower (Dextran-rich) phase and by specific staining with sodium silicotungstate. Two peaks of ATPase activity were found. One showed a pH optimum at 6.0 in the presence of 150 mM KCl and 3 mM ATP with apparent Km (ATP) and Vmax of 0.75 mM and 79 μmol (mg protein)−1 h−1, respectively. With 50 mM KCl and 7 mM ATP a pH optimum of 6.5, an apparent Km (ATP) of 6.3 mM and Vmax of 159 μmol (mg protein)−1 h−1 were determined. Both activities were specific for ATP, unspecific for monovalent cations, sensitive to sodium vanadate and Ca2+ but insensitive to azide and nitrate.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb01284.x

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A comparison of the effects of γ-ketotriazole, MCPA and carbaryl on the plasma membrane Mg2+-ATPase activity and lipid composition of rice shoots (Oryza sativa) (1993)

Ros, R. ; Cooke, D. T. ; Burden, R. S. ; [et al.]

Springer

Plant growth regulation 12 (1993), S. 67-69

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ISSN: 1573-5087

Keywords: plasma membrane ; sterols ; Mg2+-ATPase ; γ-ketotriazole ; MCPA ; carbaryl

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract The effect of γ-ketotriazole, MCPA and carbaryl on the lipid composition and ATPase activity associated with plasma membrane fractions from rice (Oryza sativa cv Bahia) shoots was investigated. With γ-ketotriazole and MCPA treatments the relative amount of δ5-avenasterol (%) was reduced in the plasma membrane, whereas with γ-ketotriazole a reduction was also found in the sitosterol content, expressed as a percentage of the total free sterol composition. The fatty acyl chain length of phosphatidylcholine fractions from MCPA-treated plants was also reduced. The plasma membrane Mg2+-ATPase activity was only stimulated in MCPA-treated plants. No changes were observed in lipid composition or ATPase activity with carbaryl treatment.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00144584

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Influence of cadmium and nickel on growth, net photosynthesis and carbohydrate distribution in rice plants (1993)

Moya, J. L. ; Ros, R. ; Picazo, I.

Springer

Photosynthesis research 36 (1993), S. 75-80

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ISSN: 1573-5079

Keywords: cadmium ; carbohydrate distribution ; nickel ; photosynthesis ; rice

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Six day old rice seedlings (Oryza sativa L. cv. Bahía) were grown for 5 or 10 days in a nutrient solution with either Cd (0.01, 0.1 mmol/l) or Ni (0.1, 0.5 mmol/l). Both Cd and Ni reduced the length of shoots and roots depending on the concentration and type of ion tested. On the other hand, the dry weight to fresh weight ratio was increased by heavy metal treatments, especially in the aerial part of 0.5 mmol/l Ni treated plants. The application of 0.1 mmol/l Cd and 0.5 mmol/l Ni to the seedlings produced an inhibition of the transport of carbohydrate reserves from the seeds from which plants were developing, to the rest of the plant. Net photosynthesis was also inhibited in treated plants. However, the total carbohydrate content in the shoots of these plants was higher than in controls. Thus, the starch, soluble sugars and sucrose content in the shoots of 0.5 mmol/l Ni treated plants was respectively up to 2.6, 2.8 and 4 times greater compared to controls. The distribution of assimilates between organs was also affected by the treatment: the carbohydrate content increased in the stem and second leaf but it was not affected or decreased in the root and third leaf. Although less evident, the effect of Cd on carbohydrate distribution and content was similar to that of Ni. The possible mechanisms involved in the abnormal carbohydrate accumulation and distribution are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00016271

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Heavy metal-hormone interactions in rice plants: Effects on growth, net photosynthesis, and carbohydrate distribution (1995)

Moya, J. L. ; Ros, R. ; Picazo, I.

Springer

Journal of plant growth regulation 14 (1995), S. 61-67

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ISSN: 1435-8107

Source: Springer Online Journal Archives 1860-2000

Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Notes: Abstract The effect of external applications of gibberellins (GA3) and abscisic acid (ABA) on the growth, carbohydrate content, and net photosynthesis of heavy metal-stressed rice plants (Oryza sativa cv. Bahía) was investigated. Treatment with cadmium (0.1 mm) and nickel (0.5 mm) inhibited rice growth and stimulated carbohydrate accumulation, especially in seeds from which seedlings were developing, stems, and first leaves. The addition of GA3 (14 μm) to the rice culture solution together with Cd or Ni partially reversed the effects of heavy metals, stimulating growth as well as mobilization of carbohydrate reserves in seeds from which seedlings had developed. GA3 increased the sugar content in roots and second and third leaves and also modified the carbohydrate distribution pattern compared with heavy metal-treated plants. In contrast to GA3, ABA (19 μm) supplied to rice cultures potentiated the effect of heavy metals, inhibiting the growth of young leaves and the translocation of storage products from source to sink organs. In addition, sugars were accumulated in roots and second leaf but not in the third leaf, the extension in length of which was also inhibited by the treatment. Net photosynthesis rates recovered transitorily in Cd-treated plants after the addition of hormones. The possible relationship between growth and carbohydrate distribution, as well as the involvement of hormones, in the response of plant to heavy metal stress is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00203115

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