ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

1

Unknown

Seawater carbonate chemistry and photosynthetic pigments and photophysiology of polar microalga Chlorella sp. (2019)

Tan, Yong Hao ; Lim, Phaik Eem ; Beardall, John ; [et al.]

PANGAEA

add to mindlist on the mindlist

Details

Publication Date: 2024-03-15

Description: Ocean acidification, due to increased levels of anthropogenic carbon dioxide, is known to affect the physiology and growth of marine phytoplankton, especially in polar regions. However, the effect of acidification or carbonation on cellular metabolism in polar marine phytoplankton still remains an open question. There is some evidence that small chlorophytes may benefit more than other taxa of phytoplankton. To understand further how green polar picoplankton could acclimate to high oceanic CO2, studies were conducted on an Antarctic Chlorella sp. Chlorella sp. maintained its growth rate (∼0.180 /day), photosynthetic quantum yield (Fv/Fm = ∼0.69) and chlorophyll a (0.145 fg/cell) and carotenoid (0.06 fg/cell) contents under high CO2, while maximum rates of electron transport decreased and non-photochemical quenching increased under elevated CO2. GCMS-based metabolomic analysis reveal that this polar Chlorella strain modulated the levels of metabolites associated with energy, amino acid, fatty acid and carbohydrate production, which could favour its survival in an increasingly acidified ocean.

Keywords: Alkalinity, total; Alkalinity, total, standard deviation; Aragonite saturation state; Bicarbonate ion; Bicarbonate ion, standard deviation; Bottles or small containers/Aquaria (〈20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Carotenoids, standard deviation; Carotenoids per cell; Cell biovolume, standard deviation; Cell density; Cell density, standard deviation; Cell surface area/cell volume, standard deviation; Cell surface area/cell volume ratio; Chlorella sp.; Chlorophyll a, standard deviation; Chlorophyll a per cell; Chlorophyta; Day of experiment; Diameter; Diameter, standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth/Morphology; Growth rate; Growth rate, standard deviation; Laboratory experiment; Laboratory strains; Light saturation; Light saturation, standard deviation; Maximal electron transport rate, relative; Maximal electron transport rate, relative, standard deviation; Maximum light utilization efficiency; Maximum light utilization efficiency, standard deviation; Non photochemical quenching, maximum; Non photochemical quenching, standard deviation; Not applicable; OA-ICC; Ocean Acidification International Coordination Centre; Partial pressure of carbon dioxide, standard deviation; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; pH, standard deviation; Photochemical quantum yield; Photochemical quantum yield, standard deviation; Phytoplankton; Plantae; Potentiometric; Potentiometric titration; Primary production/Photosynthesis; Ratio; Ratio, standard deviation; Salinity; Salinity, standard deviation; Single species; Species; Surface area; Surface area, standard deviation; Temperature, water; Treatment; Type; Volume

Type: Dataset

Format: text/tab-separated-values, 2064 data points

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

DATA PANGAEA

S·F·X

Fulltext

2

Electronic Resource

Molecular characterisation of Sargassum polycystum and S. siliquosum (Phaeophyta) by polymerase chain reaction (PCR) using random amplified polymorphic DNA (RAPD) primers (1995)

Ho, Chai-Ling ; Phang, Siew-Moi ; Pang, Tikki

Springer

Journal of applied phycology 7 (1995), S. 33-41

add to mindlist on the mindlist

Details

ISSN: 1573-5176

Keywords: molecular taxonomy ; Sargassum ; Phaeophyta ; random amplified polymorphic DNA (RAPD) ; polymerase chain reaction (PCR)

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Genomic DNA was extracted from 13 samples of Sargassum polycystum and S. siliquosum collected from various localities around Peninsular Malaysia and Singapore by using four different extraction methods. The yields and the suitability of the DNA to be used as template for the polymerase chain reaction (PCR) was compared. DNA samples were subjected to PCR analysis by using random primers. Only DNA samples that were extracted using the CTAB method were successfully amplified by random amplified polymorphic DNA (RAPD)-PCR. Five of 31 random primers (OPA02, OPA03, OPA04, OPA13 and OPM10) tested amplified sequences of DNA from the DNA samples. Reproducible, amplified products were obtained using these primers and showed some potential to be useful in discriminating individual samples within the genus, in determining relationships between species within a genus and in developing individual fingerprints for individual samples.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00003547

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

3

Electronic Resource

Environmental effects on growth and biochemical composition ofNitzschia inconspicua Grunow (1996)

Chu, Wan-Loy ; Phang, Siew-Moi ; Goh, Swee-Hock

Springer

Journal of applied phycology 8 (1996), S. 389-396

add to mindlist on the mindlist

Details

ISSN: 1573-5176

Keywords: Nitzschia inconspicua ; diatoms ; lipids ; fatty acids ; eicosapentaenoic acid ; silicate ; nitrogen ; carbon source

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The effects of nitrate and silicate levels, and carbon source on growth, biochemical composition and fatty acid composition ofNitzschia inconspicua were investigated using batch cultures. Within the range of silicate levels supplied (8.8–176 μM), no marked variations in growth trend, biochemical composition or fatty acid composition were shown. Biomass at stationary phase, ranging from 64–66 mg ash-free dry weight (AFDW) L−1, and specific growth rate (μ) based on chlorophylla (0.41–0.50 d−1) of the cultures grown within 0.3–3.0 mM NaNO3 were not significantly different. Cultures supplemented with glucose (0.1 % w/v), acetate (0.1 % w/v) or 5% CO2 attained higher biomass (85, 85, 97 mg AFDW L−1) than the control which was grown in synthetic seawater and agitated by magnetic stirring. Cells grown at 〈3.0 mM NaNO3 contained higher carbohydrate contents (14.8–21.5% AFDW) than those grown at 3.0 mM (4.0% AFDW). Lipid content increased at the expense of proteins in cells aerated with 5% CO2. The dominant fatty acids, 16:0 and 16:1, ranged from 35.7–45.0% and 36.4–45.4% total fatty acids (TFA), respectively, while the relative proportions of 20:4 (n-6) and 20:5 (n-3) ranged from 1.7–5.4% and 3.4–5.9% TFA respectively. Cultures aerated with 5% CO2 attained the highest biomass (97 mg AFDW L−1) and yield of 20:5 (n-3) (0.34 mg L−1).

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02178582

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

4

Electronic Resource

Influence of carbon source on growth, biochemical composition and pigmentation of Ankistrodesmus convolutus (1995)

Chu, Wan-Loy ; Phang, Siew-Moi ; Goh, Swee-Hock

Springer

Journal of applied phycology 7 (1995), S. 59-64

add to mindlist on the mindlist

Details

ISSN: 1573-5176

Keywords: Ankistrodesmus convolutus ; carbon source ; glucose ; fatty acids ; carotenoids

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract The unicellular chlorophyte Ankistrodesmus convolutus Corda was grown in the light using inorganic medium (Bold's Basal Medium, BBM) and BBM enriched with 0.1% w/v of glucose, sodium acetate, sodium citrate or sodium bicarbonate. Glucose supported the highest specific growth rate (µ = 0.93 d-1) and gave the highest biomass (453 mg dry weight L-1) at the time of harvest. Of four glucose concentrations (0.05, 0.1, 0.25, 0.5% w/v), best growth was attained at 0.1% w/v. At 0.5% w/v glucose, the cells had high carbohydrates but low lipids and proteins. The relative amounts of 16:0, 18:0, 18:1 and 18:2 increased at the expense of 18:3(n-3) in the carbon-supplemented cultures and at glucose concentrations higher than 0.1% w/v. Cultures grown on glucose had less chlorophyll and carotenoid contents than cultures grown on other carbon sources. Chlorophyll and carotenoid contents decreased with increasing glucose concentrations in the medium.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00003551

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

5

Electronic Resource

Studies on Gracilaria changii (Gracilariales, Rhodophyta) from Malaysian mangroves (1996)

Phang, Siew-Moi ; Shaharuddin, Shafeei ; Noraishah, Hashim ; [et al.]

Springer

Hydrobiologia 326-327 (1996), S. 347-352

add to mindlist on the mindlist

Details

ISSN: 1573-5117

Keywords: agar ; Gracilaria changii ; mariculture ; reproduction ; seasonality ; seaweed

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Gracilaria changii, recorded from Malaysia and Thailand, is one of the more abundant agarophytic seaweeds found in Malaysia. A wild population of Gracilaria changii growing in mangroves was monitored for seasonal variation in agar content and gel strength as well as spore production. Agar yield and gel strength ranged from 12 to 25% dry weight and 294 to 563 g cm−2, respectively, over a 15-month period. Gel strength but not yield was positively correlated with amount of rainfall. Cystocarps were observed throughout the study, but use of sporetraps showed that spore release peaked around July–August and January–February after the two monsoon periods. Mariculture of Gracilaria changii in shallow ponds in the mangroves, in an irrrigation canal and in a shrimp farm pond was conducted. Average growth rate of cuttings tied to monofilament lines was 3.3 ± 1.7% d−1, 8.4 ± 1.8% d−1 and 3.6 ± 1.6% d−1 respectively. Cuttings were lost to siltation, wave action, predation and heavy epiphytisation. These studies show that Gracilaria changii has potential commercial application in the agar industry.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00047829

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

6

Electronic Resource

New records of Malaysian marine algae (1994)

Phang, Siew-Moi

Springer

Hydrobiologia 285 (1994), S. 123-129

add to mindlist on the mindlist

Details

ISSN: 1573-5117

Keywords: marine algae ; Malaysia ; new records ; taxonomy

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Checklists of the marine algae of Malaysia have been published. The last checklist included the marine algae of Singapore. The checklist recorded 212 taxa for the region. This paper lists an additional 7 taxa of Chlorophyta, 18 taxa of Rhodophyta and 5 taxa of Phaeophyta, which had been left out of the last checklist. These include marine algae from Pulau Pinang, Pulau Redang and Pulau Sibu. In addition, new records of Cyanophyta (2 species), Chlorophyta (4 species), Rhodophyta (10 species) and Phaeophyta (3 species) are reported.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00005660

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

7

Electronic Resource

Studies on the production of useful chemicals, especially fatty acids in the marine diatom Nitzschia conspicua Grunow (1994)

Chu, Wan-Loy ; Phang, Siew-Moi ; Goh, Swee-Hock

Springer

Hydrobiologia 285 (1994), S. 33-40

add to mindlist on the mindlist

Details

ISSN: 1573-5117

Keywords: lipids ; fatty acids ; carbohydrates ; proteins ; culture age ; Nitzschia conspicua

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A local marine diatom, Nitzschia conspicua Grunow, was cultured in enriched synthetic seawater using flasks (agitated by magnetic stirring) and a 1.2 l fermenter. Lipids, fatty acids, proteins, carbohydrates and ash of the flask cultures were determined at various stages of growth (day 3, 5, 7, 10, 13, 15 and 17). The fermenter culture was harvested during the stationary phase for similar chemical analyses. N. conspicua attained a higher biomass concentration during the stationary phase when cultured in the fermenter (188 mg dry weight l−1) than in flasks (140–151 mg dry weight l−1). However, both systems showed similar specific growth rates based on chlorophyll-a concentration. Appreciable amounts of the essential fatty acids 20:4 (0.6–4.7% total fatty acids) and 20:5 (1.9–4.7% total fatty acids) are present in this diatom. Maximal amounts of these fatty acids were produced after 7 days' growth (i.e. 2 days after the end of the exponential phase). Lipids, fatty acids, proteins, carbohydrates and ash varied with culture age in N. conspicua.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00005651

Permalink