ISSN:
1573-8221
Keywords:
α-fetoprotein
;
ultramicromethod of detecting antigens
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract An ultramicromethod for determining α-fetoprotein (AFP), capable of detecting as little as 10−8 mg AFP in a concentration of (1–2) · 10−6 mg/ml, is suggested. The antigen is concentrated in a capillary tube by electrophoresis in polyacrylamide gel. The concentrated zone migrates into the slab of gel containing antiserum. After free diffusion of the antigen, precipitation rings are formed and their area is proportional to the quantity of antigen. The precipitates are revealed by treatment with I125-antibodies against immune serum γ-globulin followed by autoradiography.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00786443
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