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Molecular genetics of the y locus in pepper: its relation to capsanthin-capsorubin synthase and to fruit color (2000)

Popovsky, S. ; Paran, I.

Springer

Theoretical and applied genetics 101 (2000), S. 86-89

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ISSN: 1432-2242

Keywords: Keywords Capsicum ; Fruit color ; Capsanthin-capsorubin synthase

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Classical genetic studies have determined that the yellow fruit color in pepper is recessive to red in the locus y. We studied the relation of the y locus with the gene coding for capsanthin-capsorubin synthase (CCS) that synthesizes the red carotenoid pigments in the mature fruit. Cosegregation of y and CCS in populations derived from crosses between plants bearing red×white and red×yellow fruits indicated the correspondence of the two genes. We obtained indications for the occurrence of a deletion in the CCS gene in plants containing the recessive y allele. This deletion did not contain the distal 220 bp of the 3′ end of the gene. We used the CCS gene to determine the genotype of peppers with different fruit colors at the y locus. In BC1 segregants from a red×white cross, the red and peach-fruited progenies had the wild-type allele at the CCS locus, while the orange, yellow and white-fruited progenies had the mutant allele. Screening orange-fruited cultivars with CCS as well as segregation analysis of CCS in an additional red×white cross indicated two possible genotypes of the orange fruit color in this locus.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s001220051453

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Recombinant inbred lines for genetic mapping in tomato (1995)

Paran, I. ; Goldman, I. ; Tanksley, S. D. ; [et al.]

Springer

Theoretical and applied genetics 90 (1995), S. 542-548

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ISSN: 1432-2242

Keywords: Tomato ; Recombinant inbred lines ; Genetic mapping ; Molecular markers

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A cross between the cultivated tomato Lycopersicon esculentum and a related wild species L. cheesmanii yielded 97 recombinant inbred lines (RILs) which were used to construct a genetic map consisting of 132 molecular markers. Significant deviation from the expected 1:1 ratio between the two homozygous classes was found in 73% of the markers. In 98% of the deviating markers, L. esculentum alleles were present in greater frequency than the L. cheesmanii alleles. For most of the markers with skewed segregation, the direction of the deviation was maintained from F2 to F7 generations. The average heterozygosity in the population was 15%. This value is significantly greater than the 1.5% heterozygosity expected for RILs in the F7 generation. On average, recombination between linked markers was twice as high in the RILs than in the F2 population used to derive them. The utility of RILs for the mapping of qualitative and quantitative traits is discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00222001

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Quantitative trait locus analysis of a recombinant inbred line population derived from a Lycopersicon esculentum x Lycopersicon cheesmanii cross (1995)

Goldman, I. L. ; Paran, I. ; Zamir, D.

Springer

Theoretical and applied genetics 90 (1995), S. 925-932

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ISSN: 1432-2242

Keywords: Recombinant inbred line ; Quantitative trait locus ; Restriction fragment length ; polymorphism ; Tomato

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Quantitative trait loci influencing fruit traits were identified by restriction fragment length polymorphism (RFLP) analysis in a population of recombinant inbred lines (RIL) derived from a cross of the cultivated tomato, Lycopersicon esculentum with a related wild species Lycopersicon cheesmanii. One hundred thirty-two polymorphic RFLP loci spaced throughout the tomato genome were scored for 97 F8 RIL families. Fruit weight and soluble solids were measured in replicated trials during 1991 and 1992. Seed weight was measured in 1992. Significant (P〈0.01 level) quantitative trait locus (QTL) associations of marker loci were identified for each trait. A total of 73 significant marker locus-trait associations were detected for the three traits measured. Fifty-three of these associations were for fruit weight and soluble solids, many of which involved marker loci signficantly associated with both traits. QTL with large effects on all three traits were detected on chromosome 6. Greater homozygosity at many loci in the RIL population as compared to F2 populations and greater genomic coverage resulted in increased precision in the estimation of QTL effects, and large proportions of the total phenotypic variance were explained by marker class variation at significant marker loci for many traits. The RIL population was effective in detecting and discriminating among QTL for these traits previously identified in other investigations despite skewed segregation ratios at many marker loci. Large additive effects were measured at significant marker loci. Lower fruit weight, higher soluble solids, and lower seed weight were generally associated with RFLP alleles from theL. cheesmanii parent.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00222905

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Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce (1993)

Paran, I. ; Michelmore, R. W.

Springer

Theoretical and applied genetics 85 (1993), S. 985-993

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ISSN: 1432-2242

Keywords: Molecular marker ; Disease resistance ; Lettuce ; Downy mildew

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Sequence characterized amplified regions (SCARs) were derived from eight random amplified polymorphic DNA (RAPD) markers linked to disease resistance genes in lettuce. SCARs are PCR-based markers that represent single, genetically defined loci that are identified by PCR amplification of genomic DNA with pairs of specific oligonucleotide primers; they may contain high-copy, dispersed genomic sequences within the amplified region. Amplified RAPD products were cloned and sequenced. The sequence was used to design 24-mer oligonucleotide primers for each end. All pairs of SCAR primers resulted in the amplification of single major bands the same size as the RAPD fragment cloned. Polymorphism was either retained as the presence or absence of amplification of the band or appeared as length polymorphisms that converted dominant RAPD loci into codominant SCAR markers. This study provided information on the molecular basis of RAPD markers. The amplified fragment contained no obvious repeated sequences beyond the primer sequence. Five out of eight pairs of SCAR primers amplified an alternate allele from both parents of the mapping population; therefore, the original RAPD polymorphism was likely due to mismatch at the primer sites.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00215038

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