ISSN:
1573-6822
Keywords:
cell death
;
cell membrane
;
free radicals
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract Plasma membrane modifications have been widely recognized as crucial factors in cell injury and death. One of these modifications, surface blebbing, has been considered as an injury-marker associated with a series of biochemical and physiological modifications. Our study focused on the different effects of free radical-induced cell damage by quinone menadione (2-methyl-1,4-naphthoquinone) and by hyperthermic shock (45°C) on the erythroleukemic cell line K.562. Different techniques including immunofluorescence, freeze-fracturing, and electron paramagnetic resonance spectroscopy were employed. Menadione induced the formation of surface blebs, accompanied by a rearrangement of the microfilament system and changes in the distribution of plasma membrane proteins. In contrast, heat-shocked cells showed neither blebbing nor important cytoskeletal changes. Finally, the electron paramagnetic resonance results showed an increase in membrane order not specifically related to the type of free radical-induced stress. These cell death features appear to suggest the existence of two different types ofpathways for necrotic cell death: both treatments induce cell injury and eventual death by modifiting plasma membrane integrity and function. However, one involves cytoskeleton-dependent surface blebbing, whereas the other does not.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00757574
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