ISSN:
1573-0972
Keywords:
Algae
;
biosorption
;
column chromatography
;
differential pulse stripping voltammetry
;
trace metals
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract Nostoc-based biosorbents (AlgaSORBs) useful as chromatographic column-packing materials were prepared by immobilizing cyanobacteria onto solid support in three different fashions: (i) cyanobacterial biofilm (Nostoc-dimethylformamide slurry) over polymer-modified silica gel, (ii) cyanobacterial biofilm over bare silica gel, and (iii) cyanobacteria as such onto polymer-modified silica gel. The materials were characterized for their stabilities and metal sorption/elution conditions under static and dynamic equilibrations. Preconcentrated metals from a test sample were detected following `standard addition' method using a differential pulse anodic stripping voltammetric technique. All sorbents showed 100% affinity for Cd2+ ion in a multielemental sample at pH 6.9 and a flow rate of 0.5 ml/min with a preconcentration factor varying between 28- and 75-fold. The first type of AlgaSORB was also found to be selective for Cu2+ ion in multielemental analysis at pH 5.2 and a flow rate of 1.0 ml/min with a preconcentration factor of 75. The low capacity and favourable kinetics of these sorbents for Cu2+, Cd2+, Zn2+ and Pb2+ ions reflect the suitability of AlgaSORB columns for satisfactory performance in single column ion-chromatography. The polymer spacer between cyanobacterial biofilm and silica gel plays a vital role in holding the immobilized biofilm resulting in better endurance and recyclability for the first type of biosorbent.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1008931621581
Permalink