ISSN:
1573-5044
Keywords:
cereals
;
genetic variability
;
plant tissue culture
;
somaclonal variation
;
Sorghum bicolor
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Sorghum bicolor (L.) Moench, plants were regenerated from 4 to 5 month old callus cultures originally derived from seedling explants. Somaclonal variation was examined in the SC2 and SC3 generations of eight different clones plus the non-tissue cultured parent line. Characters examined included the area of the third leaf, height, tiller number, total shoot weight, seed number, grain yield, days to flowering, and chlorophyll content in the SC2 generation. Some plants were phenotypically different from the parent line in the SC2 generation. All clones were similar to the parent in third leaf area and chlorophyll content. Three clones had significant height reduction and six had significantly higher total plant matter production. Seven of the clones had significant increases in number of secondary tillers, and all had significant decreases in days to flowering. Only three clones had significant increases in grain yield and seed number; however, all the clones had smaller seeds. In subsequent studies of SC2 and SC3 generations of three somaclones, characters examined included height, tiller number, total shoot weight, and days to flower. In one of the somaclones none of the original differences in characters was maintained in two subsequent growing seasons in the SC2 or SC3 generation. The other two somaclones maintained the increased tiller number in the next growing season in both the SC2 and SC3 generations. In one of the somaclones plant height reduction occurred in the SC2 and SC3 generations. All other traits were not maintained.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040804
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