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  • 1
  • 2
    Publication Date: 2021-05-19
    Description: In order to investigate the effect of ethanol extracts of garlic (Allium sativum) and Zataria multiflora on Aeromonas hydrophila bacteria Persian sturgeon (Acipenser persicus)took the kids to the isolation and identification of bacteria , the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of bacteria Aeromonas hydrophila by garlic (Allium sativum) and Zataria multiflora hydroalchoholic extracts to determine the lethal concentrations of hydroalchoholic extracts of garlic (Allium sativum) and Zataria multiflora hydroalchoholic extracts on Persian sturgeon (Acipenser persicus)children , and also to evaluate the efficacy and determine the effective doses of the bacterium Aeromonas hydrophila extracts of in vitro and in vivo were measured.Due to the absence of the Aeromonas hydrophila identification by molecular country in the study of bacteria isolated from sturgeon disease is suspected after detection by screening DNA extraction and molecular By toward action and results by NSBI Aeromonas hydrophila bacteria and Authentication Code NSBI was recorded in Gen Bank JX987090 . Based on studies done in vitro (in vitro) in this study, the minimum inhibitory concentrations (MIC) Aeromonas hydrophila bacteria by extracts of garlic and thyme and arrange 1 mg/ml, 0.25mg/ml and the minimum bactericidal concentration (MBC) of bacteria Aeromonas hydrophila by the extracts, respectively , and 2mg/ml, 0.5mg/ml. Study on lethal concentration (LC50) of Zataria multiflora hydroalchoholic extract on fingerlings of Persian sturgeon showed that during 96h and 1h, the LC50 was 766.65 and 9933.44 mg/L, respectively. Also, LC50 of garlic extract during 96h and 1h was 1279.97 and 12624.08 mg/L, respectively. Investigation on white blood cells (WBC) showed significant difference in lymphocyte and neutrophil numbers in different treatments (P〈0.05). But, there was no significant difference in monocyte and eosinophil numbers in different treatments (P〉0.05). In this study, concentrations ranging from 400 to 1,000 mg/ml of hydroalchoholic extracts of Zataria multfor treating Persian sturgeon (Acipenser persicus) infected with the Aeromonas hydrophila by intraperitoneal injection were determined. Based on the results of the concentration of the extract to 800 mg/ml during shower hour was determined . Assay to determine the concentration of garlic extract on the bacteria in the Persian sturgeon (Acipenser persicus)concentration range of 600 to 1200 mg/ml determine the effective concentration of extract equivalent to 1,000 mg/ml during shower hour was calculated. Study on the pictures taken out from sections of gill, liver and kidny of Persian sturgeon fingerlings (Acipenser persicus) showed that in different doses of garlic (Allium sativum) and Zataria multiflora hydroalchoholic extracts the teretment grups were examined and some microscopic damages observed. They are hyperemia, adhesion in the gill filaments, cell necrosis, melanin pigments in gill primary filaments, cloudy swelling of hepatocytes, liver necrosis, hyperemia and increase in melanin pigments and melano macrophage centers in liver, glomerular changes such as congestion and blocked the dilation of Bowman's space , bleeding, cell necrosis , cloudy swelling of the in kidny.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Allium sativum ; Zataria multiflora ; Aeromonas hydrophila ; Acipenser persicus ; Aeromonas hydrophila ; Kidny ; Fish ; Garlic ; Fingerling
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 82pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Acipenser persicus, one of the native Species living in the southern part of the Caspian Sea, has high Percentage of larval mortality. Therefor to decrease the rate of mortality of larvae survival and to increase to regenerate properly during the active feeding process of fish larvae, while under artificial reproduction they were fed withe fresh water rotifer. Four types of feeding treatments were applied as follow :Treatment 1: Similar to normal process , first withe Artemia cyst then withe Daphnia Treatment 2: Mixture of Artemia, Rotifer and Daphnia; Treatment 3 freshwater Rotifera ;Treatment 4: fresh water Rotifer enriched with Vitamin C (Ascorbic acid- 6- Palmytat ).Three replicates were used for each treatment. In the process of each observation 45 larvae in containers withe 100 liter capacity filled with 30 liters of water , after 8 days of external feeding process, were inspected closely, during which , they were under biometric measurement once every two days. Larvae were fed Four times a day at a rate of 25 % of their body weight. The average temperature estimation was 22/5 ±0/5 centigrade, pH of water being 8/5 ± 0/1 while Oxygen proved to be 9/58 ± 0/2 mg/l . At the end of the observation, the profill fatty acids of the remains were studied .The growth factors, the mouth size of the larvae and the expansion of the mouth size compared to the total length of the larvae during the whole observation were inspected. Credibility of data designated was examined by Shapiro Wilks test. Specific growth rate (SGR), weight growth (WG) and feed conversion ratio (FCR) were contemplated by one- way analysis test, and significant difference Tukey's test. The most notable diversity was evident between treatment 2 , whose indicative factors were in the following order : 4/ 65 ± 0/06, 45/18 ± 0/66, 4/48 ± 0/07 and treatment 4 whose indicative factors showed the following results: 10/47 ± 0/04, 124/42 ± 0/62, 1/51 ± 0/008.For the determination of fatness rate and weight gains, Kruskal Valis and Man vit ni test were used .In this part of study , the highest diversity fatness appeared to be between treatment 4 ,reflecting 0/79±0/07 and treatment 2 showing the result 0/62±0/05.The highest gain was related to treatment 4, whit the rate of 99/33 ± 0/68 mgr and the lowest gain occurred in treatment 2 white the rate 62/36 ± 0/65 mgr . This study also revealed that, the survival ratio connected with treatments 3 and 4 , compared treatments 1 and 2, were significantly different. The profile of fatty acids also showed much higher percentage of PUFA and HUFA in treatments 3 and 4 , than those in other treatments. Furthermore Brachionus calyciflorus proved to be a proper food supply for larvae. Therefore by building suitable pools next to the work side, other kinds of species fresh water rotifer could also be used as food improvements for feeding larvae.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Brachionus calyciflorus ; Acipenser persicus ; Fatty acid ; Larvae culture ; Vitamin C ; Larval survival ; Species ; Mortality ; Feeding ; Artemia ; Cyst ; Ascorbic acid ; Oxygen ; Growth ; SGR ; FCR ; Fresh water
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 78pp.
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  • 4
    Publication Date: 2021-05-19
    Description: We collected sturgeon specimens from seven fishing stations operating 30 to 35 Km away from each other in Guilan province over the years 1999 MD 2000. Trawl nets six meters long were drawn using motorboats at each station, repeated three times for each season. Among the studied premature sturgeons, 121 Acipenser persicus specimens with an average age of less than two years and a length of 10 to 49.9 ± 0.77 cm showed full stomachs (57.17 % fullness). Among the mature specimens with an average age of 11 years and a length range of 90 to 194 cm, only three showed full stomachs (11.54 % fullness). The highest gut index in this study belonged to premature A. persicus. A one-way analysis of variance showed no significant difference in condition factor and gastro-somatic index among the different length groups (P〈0.05). We found that the food consumed by premature A. persicus was comprised of eight genera of benthic invertebrates Hypania sp., Hypaniola sp., Pterocuma sp., Stenocuma sp., Abra ovata, Paramysis sp., Gammarus sp. and Nereis sp. Adult sturgeons were found feeding mainly on fish species belonging to Gobidae, Atherinidae and Clupeidae families. The main preys of premature A. persicus were polychaet worms Hypania sp. and Hypaniola sp. belonging to the class Sedantaria. Crustaceans Stenocuma sp. and Pterocuma sp. of the class Cumacea were also seen in the diet of the premature sturgeons. The main preys of adult A. persicus were fishes belonging to the family Gobiidae and secondary prey were fishes of the family Atherinidae.
    Description: Published
    Keywords: Acipenseridae ; Acipenser persicus ; Hypania sp. ; Hypaniola sp. ; Pterocuma sp. ; Stenocuma sp. ; Paramysis sp. ; Gammarus sp. ; Nereis sp. ; Atherinidae ; Feeding ; Benthic
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.37-48
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  • 5
    Publication Date: 2021-05-19
    Description: Nereis diversicolor worms were collected from the Anzali lagoon during the years 2004-2006 using Van Veen grab sampler with a surface area of 400cm super(2). Water salinity, temperature and total organic matter (TOM) of sediments in the sampling region was determined. The worms were maintained in 0.5 tons (1x1m2 super(2)) tanks until they reached a weight of 200-300mg. Sexual maturity in the samples was attained at 4-6C and spawning occurred at approximately 16C. The first gametes were observed after the temperature increased from 6 to 16C. Sexual maturity was studied at various salinities (0.5, 5, 12, and 15 ppt). Results indicated that the worm attains sexual maturity at salinity 15ppt in a shorter period as compared to other salinities. No significant differences were observed between sexual maturity attained at salinities 12 and 15ppt (P〉0.05). The effect of light and photoperiod in synchronizing reproduction in male and female N. diversicolor was also studied. We observed that reproduction behavior in adult worms increased for a period of one week at the end of each month after they were exposed to a prolonged photoperiod (L:D 16:8) followed by a period of dim light. Mature males crawled around outside the tubes in search of a female. In the presence of a mature female, the males released sperm directly in front of the female tube. Soon after, there was an increase in the activity of the females which showed intense ventilation movements. The female worms with their eggs already released began to carry the sperm into their tube. The ripe females nearby which had not yet laid eggs in their tubes released their gametes a few minutes after the male and began to carry sperm into their tubes. The fertilized eggs remained inside the tube protected by the female. The benthic larvae developed fast, feeding on the mucus and microbial film on the tube wall. After 10 to 14 days, larvae began to leave the tube. A few days later the females died.
    Description: Published
    Keywords: Salinity ; Photoperiods ; Reproductive cycle ; Nereis diversicolor ; Sexual maturity ; Temperature ; Reproduction ; Spawning ; Biological fertilization ; Freshwater
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.11-20
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  • 6
    Publication Date: 2021-05-19
    Description: The marine survey for sturgeon stock assessment was conducted in summer, winter and spring in the years 2006 and 2009 to estimate the relative and absolute abundance and percentage composition of each species in the Guilan, Mazandaran and Golestan Provinces. This survey was carried out in the Iranian waters of the Caspian Sea on board the Sisara2 and Guilan vessels using trawl nets at 2-100 m depths. Trawling was carried out in 85 stations that were selected using a stratified random design. The number of stations in each scope was based on the area of the scope in terms of the total area. Trawling and sampling in shallow water up to 10 m were carried out using 9 m trawl nets whereas 24.7 m trawl nets were used for depths more than 10 m. Trawl surveys were carried out in the daytime. Trawling velocity was kept at 2.5-3 kts and trawls lasted half an hour in order to calculate abundance, and biomass of sturgeons using the swept area method. Catch per unit area (CPUA) in the winter 2006 survey was 3853 specimens nm2 , in the summer and winter 2007 survey was 1854 , 2912 specimens nm2 at depths less than 10 m respectively . CPUA for sturgeons in spring 2008 survey was 2103 specimens nm2 at depths less than 10 m and 393 specimens nm2 at depths greater than 10 m (10-100 m depth). These values in the winter 2008 survey dropped to 44 specimens nm2 at depths at depths above 10 m. CPUA for sturgeons in the spring 2009 survey was 300 specimens nm2 at depths less than 10 m and 307 specimens nm2 at depths greater than 10 m. In all the surveys conducted CPUA for A. persicus was higher than that for the other sturgeon species. Based on the calculations carried out in the marine survey in winter 2006 the estimated absolute abundance for sturgeons was about 2977.363 thousand. The total biomass of sturgeon was estimated as 131.713 tons. In the summer 2007 survey total abundance was estimated 1432.398 thousand, and total biomass of sturgeons was estimated at about 312.161 tons. In the winter 2007 survey total abundance for sturgeons was estimated at about 2250.105 thousand, and total biomass was estimated 578.08 tons. In the spring 2008 survey total abundance was estimated at about 3002.832 thousand. The total biomass was estimated at about 2533.318 tons .In the winter 2008 survey total abundance was estimated at about 152.722 thousand, and total biomass in winter 2008 was estimated 170.540 tons. Total abundance in spring 2009 survey was 1310.232 thousand and total biomass was estimated at 2019.tons. Investigation of stomach content of sturgeon Acipenser persicus caught under 10m depth in 2006 to 2007 surveys showed that there is significant difference in the consumed food. Polychaeta is the major food consumed and crustacean an the minor one(P〉0.05).Also no new types of food( such as bony fishes or benthic) have been observed in food chain of Acipenser persicus only the food consumption rate has been related to the season of year and increases or decreases in warm or cold seasons, respectively For physiological study and determination of sexual maturation stages in sturgeon,119 gonad sampling prepared. The results showed that 63 % of fishes were females and 37 % of them were males. Gill microscopic study shows complications such as hyperplasia, curvature, adhesion, embowed, shorting and lengthen of secondary filaments and fraught bloody. Microscopic study on liver indicates signs of cloudy inflammation, fatty degeneration, dispersion of billed secretions and cell atrophy. The population genetic structure of Persian sturgeon (Acipenser persicus) in Sefidrood and Gorganrood rivers watershed analyzed based on microsatellite markers during sturgeons assessment in 2006-2008. Results showed that Acipenser persicus in two region of south part of Caspian Sea are two independent populations.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Stock assessment ; Sturgeon ; Acipenser persicus ; Diet ; Gills ; Sex ; Microsatellite ; Population ; Genetic ; Survey ; Species ; Sampling ; Trawling ; CPUA ; A. persicus ; Biomass ; Polychaeta ; Maturation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 154pp.
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  • 7
    Publication Date: 2021-05-19
    Description: Feeding behavior in Acipenser persicus and Acipenser stellatus in summer and winter was studied from 2004 to 2006 simultaneously with the marine survey for sturgeon stock assessment. Bottom trawls (9m) on board the research vessel Sisara 2 (50 hsp) were used to collect specimens at depth below 10m, while specimens at depths more than 10m were collected using bottom trawls (24.7m) on board the Guilan Research Vessel (1000 hsp). Examination of stomach contents of 135 A. persicus and 80 A. stellatus specimens revealed that the food spectrum was composed of bony fishes (Neogobius sp., Atherina sp., Clupeonella cultriventris), invertebrates belonging to the family Ampharitidae (polychaeta worms including Hypanai sp. and Nereis diversicolor), various crustaceans (Gammarus sp. and Paramysis sp.) and the bivalved mollusc, (Abra ovate). Significant differences were detected in the frequency of prey items in the different seasons studied. Also significant differences (P0.05).
    Description: Published
    Keywords: Food organisms ; Stomach content ; Anadromous species ; Bottom trawls ; Stock assessment ; Trawl nets ; Neogobius ; Polychaeta ; Nereis diversicolor ; Acipenser stellatus ; Paramysis ; Acipenser ; Acipenser persicus ; Abra ; Atherina ; Brackish ; Feeding behaviour ; Marine molluscs ; Taxonomy ; Marine crustaceans ; Winter ; New species
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.13-26
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  • 8
    Publication Date: 2021-05-19
    Description: We collected sturgeon specimens from seven fishing stations operating 30 to 35 Km away from each other in Guilan province over the years 1999- 2000. Trawl nets six meters long were drawn using motorboats at each station, repeated three times for each season. Among the studied premature sturgeons, 121 Acipenser persicus specimens with an average age of less than two years and a length of 10 to 49.90.77 cm showed full stomachs (57.17 % fullness). Among the mature specimens with an average age of 11 years and a length range of 90 to 194 cm, only three showed full stomachs (11.54 % fullness). The highest gut index in this study belonged to premature A. persicus. A one-way analysis of variance showed no significant difference in condition factor and gastro-somatic index among the different length groups (P〈0.05). We found that the food consumed by premature A. persicus was comprised of eight genera of benthic invertebrates Hypania sp., Hypaniola sp., Pterocuma sp., Stenocuma sp., Abra ovata, Paramysis sp., Gammarus sp. and Nereis sp. Adult sturgeons were found feeding mainly on fish species belonging to Gobidae, Atherinidae and Clupeidae families. The main preys of premature A. persicus were polychaet worms Hypania sp. and Hypaniola sp. belonging to the class Sedantaria. Crustaceans Stenocuma sp. and Pterocuma sp. of the class Cumacea were also seen in the diet of the premature sturgeons. The main preys of adult A. persicus were fishes belonging to the family Gobiidae and secondary prey were fishes of the family Atherinidae
    Description: Published
    Keywords: Feeding ; Sturgeon ; Acipenseridae ; Specimens ; Acipenser persicus ; Mature ; A. persicus ; Hypania sp. ; Hypaniola sp. ; Pterocuma sp. ; Stenocuma sp. ; Abra ovata ; Paramysis sp. ; Gammarus sp. ; Nereis sp. ; polychaet ; Gobiidae ; Atherinidae
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.37-48
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  • 9
    Publication Date: 2021-05-19
    Description: The operations of this project commenced with the capture and tagging of four spawners in the spring of 2006 and 2007 in the Sepidrud River. Three male Acipenser persicus spawners and one male A. stellatus spawner were collected by beach seine (120 m long, 6 m high and mesh size of 10 and 15 cm) and waiting nets. Spawners were tagged with radio transmitters with a frequency between 150-151 MHz. These transmitters were used for the first time in Iran on fish. Signals were received using a radio receiver model WTI-1000 (Wildlife Track Inc.) with Yagi antennae and a Telex ProAir 1500 earphone. Tagged spawners were tracked from the land (along the river margin) and in water in motor boats. The aim of this study was to study the potential for migration and natural reproduction in sturgeons in the Sepidrud River. Water temperature during the study period varied between 12-26 ֯C in the first year and between 16-22 ֯C in the second year of the study. The A. stellatus spawner weighed 6 kg while the A. persicus spawners weighed between 11 to 25 kg. Sperm motility of A. stellatus spawner was 39%, sperm density was 0.42x109 ml and motility time was 15 sec., while in A. persicus spawners, sperm motility varied between 50-65%, sperm density was between 0.500x109 to 0.865x109 ml and motility time of sperms varied between 20-42 sec. Tracking tagged spawners revealed that A. stellatus spawners moved at an average speed of 1100 m h-1 (influenced by the strong currents in the river) while A. persicus spawners moved downstream at an average speed of 90 m h-1. Results of the present study indicate the presence of potential barriers such as water temperature, river water flow, physiological conditions of spawners in terms of sexual maturity, and the presence of fishers and fishing gear, for the migration of sturgeon spawners in the Sepidrud River. However the spawners were able to escape the various fishing gear set up in the Sepidrud River and return to the sea, indicating the possibility of natural reproduction in this river.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Physiological ; Radio transmitters ; Migration ; Sturgeons ; Spawners ; Male ; Acipenser persicus ; A. stellatus ; Temperature ; A. persicus ; Sperm ; Motility ; Sexual maturity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
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  • 10
    Publication Date: 2021-07-15
    Description: Phytoplankton and benthic organisms diversity and density were studied in six sturgeon rearing ponds in the Yousefpour and Shahid Beheshti hatcheries from 22 April to 1 July 2001. We used Ruttner sampler, plankton net with a mesh size of 50 microns and Ekman grab to sample the organisms on a weekly basis and fixed the samples in formalin solution 4% and then identified 22 genera of phytoplanktons for the three ponds in the Yousefpour hatchery and another 21 genera for the three ponds in the Shahid Beheshti hatchery. Planktons from the phyra Chlorophyta, Cyanophyta, Chrysophyta, Cryptophyta and Euglenophyta were present in the ponds. Schroederia sp. of the phyla Chlorophyta was the dominant species identified. We observed 2 x 106 and 1 x 106 cells of phytoplanktons per milliliter of pond water in the Yousefpour and Shahid Beheshti hatcheries respectively. The zooplankton phyla Arthropoda and Rotifera were represented with 9 genera in the two hatcheries showing a density of 36937 and 46603 zooplanktons per liter of water in the Yousefpour and Shahid Beheshti hatcheries respectively. Insect larvae and oligochaeta worms were the benthic organisms common to both hatcheries and mollusks were present only in the Yousefpour hatchery. The average benthic biomass was 1.58±0.19 and 2.16±0.75 g/m2 in the Yousefpour and Shahid Beheshti hatcheries respectively. The condition factor in sturgeon fingerlings of the Yousefpour hatchery varied from 0.294 to 0.554 while that of sturgeon fingerlings of the Shahid Beheshti hatchery were 0.297 to 0.6.
    Keywords: Aquaculture ; Ecology ; Sturgeon fry ; Plankton ; Benthic organisms ; Condition factor ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 51-64
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