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  • 1
    Electronic Resource
    Electronic Resource
    A Light- and Electron-Microscopical Study of Protacanthamoeba caledonica n. sp., Type-Species of Protacanthamoeba n. g. (Amoebida, Acanthamoebidae) (1981)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 28 (1981), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In its amoeboid stage, Protacanthamoeba caledonica n. g., n. sp. closely resembles the genus Acanthamoeba, on both light- and electron-microscopical levels, including possession of a centrosphere with a plaque-shaped centriole-like body. The cyst wall differs from that of Acanthamoeba in lack of preformed exit pores and in fine structure; the occasional apparent division into exocyst and endocyst is due to irregular splitting. The strain isolated from a Scottish estuary did not grow at 37°C and did not grow normally on agar made with 25% sea water, but cysts remained viable after a week in full-strength sea water. Protacanthamoeba n. g. is distinguished from Acanthamoeba on the basis of cyst structure, but it is assigned to the family Acanthamoebidae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1981.tb02807.x
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  • 2
    Electronic Resource
    Electronic Resource
    Re-Definition of the Genus Acanthamoeba with Descriptions of Three Species (1967)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 14 (1967), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Ten strains of Acanthamoeba from freshwater habitats were isolated in clonal cultures. Studies were made of trophic structure, nuclear division, cyst structure, some aspects of cytochemistry, and other characteristics. One strain was identified as A. castellanii (Douglas, 1930), one as A. astronyxis (Ray and Hayes, 1954), and 8 as A. polyphaga (Puschkarew, 1913). Strains of Acanthamoeba isolated by other workers were also examined comparatively.The pattern of nuclear division in all strains resembled that in metazoan cells, with the exception that centrioles were never found. Trophic amoebae had a PAS-positive surface outline. Cyst walls were strongly PAS-positive and also gave a positive test for cellulose with zinc chloroiodide.The genus Acanthamoeba Volkonsky, 1931 is re-defined, being distinguished from Hartmannella Alexeieff, 1912, emend. Volkonsky, chiefly by the formation of tapering, hyaline pseudopods (acanthopodia) and by a cyst made up of an ectocyst and a polyhedral or stellate endocyst, with excystment by removal of opercula. Other characteristics found in all strains include a distinctive food cup, the presence of many small refractile globules in the cytoplasm of trophic amoebae, and a cyst wall containing cellulose. The degree of spindle convergence, employed by Volkonsky as a generic criterion, was unusable.Differential diagnoses based principally on cyst structure are offered for A. castellanii, A. astronyxis, and A. polyphaga. The strain previously called Mayorella palestinensis Reich, 1933 is a distinct species of Acanthamoeba.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1967.tb02066.x
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  • 3
    Electronic Resource
    Electronic Resource
    Generic Criteria for Flabellula, Rugipes and Hyalodiscus, With Descriptions of Species (1968)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 15 (1968), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Six strains of amoebae isolated from freshwater habitats and having broad, flattened locomotive forms were studied in clonal cultures, with attention to trophic structure, nuclear division, cyst structure in those strains which formed cysts, some aspects of cytochemistry, and other characteristics.Three strains were found to belong to the genus Flabellula, one being identified as F. platypodia (Gläser, 1912) and the other 2 as F. mira Schaeffer, 1926. The new species Rugipes placidus is described. One strain, identified as belonging to the species originally called Amoeba actinophora Auerbach, 1855, is re-described as Hyalodiscus actinophorus, and a similar strain is described as H. actinophorus var. minor n. var.All 3 strains of Flabellula had the unusual type of mitotic figure reported by Gläser for F. platypodia but not previously studied with the Feulgen technic. The genus Rugipes has the mitotic pattern more common to small amoebae and is distinguished from Flabellula partly on that basis. Both strains of Hyalodiscus have division figures of this common type.Of the strains included in this investigation, only R. placidus and H. actinophorus formed cysts in culture. The cysts of neither species were viable after one year at room temperature and humidity.The status of Rugipes as distinct from Flabellula is discussed. The genus Hyalodiscus is interpreted in a manner compatible with the original definition of Hertwig and Lesser and is included within the order Amoebida. The strains of this genus isolated have a doubly contoured, strongly PAS-positive covering over the endoplasmic hump.Some criteria for the taxonomy of amoebae are evaluated in the light of work reported in this and 2 previous papers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1968.tb02084.x
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  • 4
    Electronic Resource
    Electronic Resource
    Two Marine Species of Flabellula (Amoebida, Mayorellidae) (1971)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 18 (1971), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Two species of amoebae identified as Flabellula citata Schaeffer, 1926, and F. calkinsi (Hogue, 1914) were isolated from saltwater habitats on the Maine coast. Both species showed the locomotive form and behavior of the genus Flabellula emend. Bovee, 1965. Both showed a mitotic pattern distinct from that of the genus Vannella Bovee, 1965, supporting Bovee's subdivision of Schaeffer's genus Flabellula. Preparations of F. calkinsi had an unusually high proportion of cells with 2 or more nuclei. F. calkinsi is a voracious cannibal. Neither F. citata nor F. calkinsi formed cysts in culture. Both species could be cultivated on media made with highly dilute sea water, but neither survived in a medium equivalent to a freshwater medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1971.tb03277.x
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  • 5
    Electronic Resource
    Electronic Resource
    Cultivation and Lyophilization of Mastigina sp. (1970)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 17 (1970), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS Mastigina sp. is an amoeboid flagellate isolated from pine frass collected in the Guadarrama Mountains in Spain. It feeds on bacteria and yeasts. It prefers yeasts that produce extracellular polysaccharides, and the 2 species that have been used predominantly for cultivation of the flagellate are Pachysolen tannophilus and Hansenula holstii. Mastigina sp. is easily isolated in axenic culture and grows abundantly therein. Its locomotive form, averaging 27 μ in length, resembles that of a limax amoeba, with a vesiculate nucleus at the anterior end. Cells are capable of simultaneous movement by pseudopodia and flagella. It develops rapidly on dead or living yeast cells in shaken cultures and the trophozoites may convert quantitatively to cysts. The cysts remain viable for long periods of time in refrigerated suspensions and in the lyophilized state. They are spherical or ovoid and smooth-walled cysts; the trophozoite emerges from them by breaking the wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1970.tb04720.x
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  • 6
    Electronic Resource
    Electronic Resource
    Two New Species of Paramoeba from Maine (1970)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 17 (1970), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS Four clonal strains of amoebae isolated from salt-water habitats of the Maine coast show the characteristic Nebenkörper or paranucleus of the genus Paramoeba Schaudinn, 1896, this being the first report of free-living members of that genus from North America. The isolates are identified as 2 species, P. pemaquidensis n. sp. and P. aestuarina n. sp. In form and mitotic pattern, these 2 species resemble members of the family Mayorellidae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1970.tb04706.x
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  • 7
    Electronic Resource
    Electronic Resource
    Platyamoeba stenopodia. n. g., n. sp., a Freshwater Amoeba (1969)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 16 (1969), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Platyamoeba stenopodia n. g., n. sp., isolated from a lake in Alabama, is an amoeba with flattened form, usually more than 2.5 × as long as broad, the anterior half hyaline and the posterior half granular, with a single vesiculate nucleus at the posterior edge of the hyaline region. It forms a transitory floating stage with slender, hyaline pseudopods. Its cyst is spherical, uninucleate, with a smooth inner wall and a thinner, closely applied outer wall, which is often slightly wrinkled. Length of locomotive amoeba 15–36 μ diameter of cyst 7–11.5 μ Reproduction by binary fission with mesomitotic nuclear division, the nuclear membrane disappearing in prophase. The genus Platyamoeba is erected for amoebae including this organism; the type species is P. placida.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1969.tb02296.x
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  • 8
    Electronic Resource
    Electronic Resource
    Taxonomic Criteria for Limax Amoebae, with Descriptions of 3 New Species of Hartmannella and 3 of Vahlkampfia (1967)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 14 (1967), S. 0 
    Details
    ISSN: 1550-7408
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: SYNOPSIS. Seven species of limax amoebae were isolated into clonal, monoxenic cultures with Aerobacter aerogenes from material collected from freshwater habitats. Studies were made of their trophic structure, nuclear division, cyst structure, some aspects of cytochemistry, and other characteristics. Six new species are described: Vahlkampfia inornata, V. avara, V. jugosa, Hartmannella limacoides, H. vermiformis, and H. exundans. The well-known species Naegleria gruberi (Schardinger, 1899) is re-described on the basis of 8 strains; its flagellated phase was found to be biflagellate, with rare exceptions.A correlation exists between the manner of locomotion and the pattern of nuclear division in the limax amoebae in the family Vahlkampfiidae and those in the genus Hartmannella.Trophic amoebae of all species had a PAS-positive surface layer, altho results with H. vermiformis and H. exundans were less definite than with other species. All species except H. limacoides formed cysts in culture. The cyst walls of all cyst-forming species were strongly PAS-positive, but results of the zinc chloroiodide test for cellulose were negative with the method used.The genus Hartmannella Alexeieff, 1912, is re-defined to include those species which assume a simple, monopodial limax-like form during locomotion and have nuclear division similar to that of metazoan cells and to distinguish it from the genus Acanthamoeba Volkonsky, 1931.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1550-7408.1967.tb02036.x
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  • 9
    Electronic Resource
    Electronic Resource
    Paramoeba: A Common Marine Genus (1973)
    Springer
    Hydrobiologia 41 (1973), S. 183-188 
    Details
    ISSN: 1573-5117
    Keywords: Amoebae ; Nebenkörper ; North Sea ; Paramoeba ; Sarcodina ; zoogeography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Amoebae of the genus Paramoeba Schaudinn, 1896, have been found in British waters, further attesting to the wide distribution and common occurrence of these unusually interesting marine protozoa. The British strains belong to the species P. pemaquidensis Page, 1970, first described from the Atlantic coast of North America. The Nebenkörper appears to be characteristic of a morphologically and systematically homogeneous group of amoebae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016444
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  • 10
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    Paramoeba: A Common Marine Genus (1973)
    Springer
    Details
    Publication Date: 1973-03-01
    Print ISSN: 0018-8158
    Electronic ISSN: 1573-5117
    Topics: Biology
    Published by Springer
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