Publication Date:
2014-08-16
Description:
Artificial DNA and RNA structures have been used as scaffolds for a variety of nanoscale devices. In comparison to DNA structures, RNA structures have been limited in size, but they also have advantages: RNA can fold during transcription and thus can be genetically encoded and expressed in cells. We introduce an architecture for designing artificial RNA structures that fold from a single strand, in which arrays of antiparallel RNA helices are precisely organized by RNA tertiary motifs and a new type of crossover pattern. We constructed RNA tiles that assemble into hexagonal lattices and demonstrated that lattices can be made by annealing and/or cotranscriptional folding. Tiles can be scaled up to 660 nucleotides in length, reaching a size comparable to that of large natural ribozymes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Geary, Cody -- Rothemund, Paul W K -- Andersen, Ebbe S -- New York, N.Y. -- Science. 2014 Aug 15;345(6198):799-804. doi: 10.1126/science.1253920.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for DNA Nanotechnology, Interdisciplinary Nanoscience Center, and Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark. ; Bioengineering, Computer Science, and Computation and Neural Systems, California Institute of Technology, Pasadena, CA 91125, USA. ; Center for DNA Nanotechnology, Interdisciplinary Nanoscience Center, and Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark. esa@inano.au.dk.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25124436" target="_blank"〉PubMed〈/a〉
Keywords:
DNA/chemistry/genetics
;
DNA-Directed RNA Polymerases/metabolism
;
*Nanostructures
;
*Nucleic Acid Conformation
;
RNA/*chemistry/genetics/*ultrastructure
;
Software
;
Templates, Genetic
;
*Transcription, Genetic
;
Viral Proteins/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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