Publication Date:
1983-09-30
Description:
The near identity of the partial amino acid sequence of human platelet-derived growth factor (PDGF) and that predicted for p28sis, the putative transforming protein of the simian sarcoma virus (SSV), suggests expression of a growth factor activity may be central for transformation by SSV. It is now reported that SSV-transformed cells but not control cells contain a growth factor activity that is identical to PDGF in immunoassay, in mitogenic dose response, and in specific mitogenic activity. The protein immunoprecipitated by antiserum to human PDGF has an apparent molecular weight of 20,000, identical to that of p20sis, the putative intracellular degradation product of p28sis. The results support the concept that expression of a PDGF-like molecule, which appears to be the product of the viral-sis gene, is responsible for the abnormal regulation of growth is SSV-transformed cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Deuel, T F -- Huang, J S -- Huang, S S -- Stroobant, P -- Waterfield, M D -- New York, N.Y. -- Science. 1983 Sep 30;221(4618):1348-50.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6310754" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
*Cell Transformation, Viral
;
Cross Reactions
;
DNA Replication/drug effects
;
*Genes, Viral
;
Growth Substances/*genetics/immunology
;
Mice
;
Molecular Weight
;
Peptides/*genetics/immunology
;
Platelet-Derived Growth Factor
;
Retroviridae/*genetics
;
Sarcoma Virus, Woolly Monkey/*genetics
;
Sarcoma, Experimental/*physiopathology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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