Publication Date:
1993-07-02
Description:
Type I diabetes [insulin-dependent diabetes mellitus (IDDM)] is an autoimmune disease associated with the destruction of pancreatic beta cells. Serum from patients with IDDM increased L-type calcium channel activity of insulin-producing cells and of GH3 cells derived from a pituitary tumor. The subsequent increase in the concentration of free cytoplasmic Ca2+ ([Ca2+]i) was associated with DNA fragmentation typical of programmed cell death or apoptosis. These effects of the serum were prevented by adding a blocker of voltage-activated L-type Ca2+ channels. When the serum was depleted of immunoglobulin M (IgM), it no longer affected [Ca2+]i. An IgM-mediated increase in Ca2+ influx may thus be part of the autoimmune reaction associated with IDDM and contribute to the destruction of beta cells in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Juntti-Berggren, L -- Larsson, O -- Rorsman, P -- Ammala, C -- Bokvist, K -- Wahlander, K -- Nicotera, P -- Dypbukt, J -- Orrenius, S -- Hallberg, A -- New York, N.Y. -- Science. 1993 Jul 2;261(5117):86-90.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Rolf Luft Center for Diabetes Research, Department of Endocrinology, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7686306" target="_blank"〉PubMed〈/a〉
Keywords:
3-Pyridinecarboxylic acid,
;
1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl
;
ester/pharmacology
;
Animals
;
Apoptosis
;
Calcium/*metabolism
;
Calcium Channels/drug effects/*metabolism
;
DNA Damage
;
Diabetes Mellitus, Type 1/*immunology
;
Humans
;
Immunoglobulin M/*physiology
;
Islets of Langerhans/drug effects/*metabolism
;
Membrane Potentials
;
Mice
;
Pituitary Neoplasms/metabolism
;
Tumor Cells, Cultured
;
Verapamil/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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