Publication Date:
1992-01-24
Description:
The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Harley, V R -- Jackson, D I -- Hextall, P J -- Hawkins, J R -- Berkovitz, G D -- Sockanathan, S -- Lovell-Badge, R -- Goodfellow, P N -- MC_U117562207/Medical Research Council/United Kingdom -- New York, N.Y. -- Science. 1992 Jan 24;255(5043):453-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Human Molecular Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1734522" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
DNA-Binding Proteins/*metabolism
;
Female
;
Gene Expression Regulation
;
Humans
;
In Vitro Techniques
;
Male
;
Mice
;
Molecular Sequence Data
;
*Nuclear Proteins
;
Oligonucleotide Probes
;
Recombinant Proteins/metabolism
;
Sequence Alignment
;
Sex-Determining Region Y Protein
;
Transcription Factors/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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