Publication Date:
1990-05-11
Description:
It is a challenge to construct synthetic immunogens that elicit antibodies (Abs) both directed to conformational epitopes and specific for a complex protein like human choriogonadotropin (hCG). A monoclonal antibody specific for hCG bound to regions around Lys45 of the alpha subunit (hCG alpha) and Asp112 of the beta subunit (hCG beta). A peptide comprising residues 46 to 55 of hCG alpha and residues 106 to 116 of hCG beta elicited Abs in rabbits that were directed to a discontinuous epitope and were specific for hCG. These Abs inhibited the binding of hCG to its receptor. Thus, a synthetic immunogen can mimic a conformational-specific epitope and can be useful for vaccine development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bidart, J M -- Troalen, F -- Ghillani, P -- Rouas, N -- Razafindratsita, A -- Bohuon, C -- Bellet, D -- New York, N.Y. -- Science. 1990 May 11;248(4956):736-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Departement de Biologie Clinique, Institut Gustave-Roussy, Villejuif, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1692160" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Antigen-Antibody Complex
;
Chorionic Gonadotropin/genetics/*immunology
;
Chorionic Gonadotropin, beta Subunit, Human
;
Epitopes/*analysis/genetics
;
Glycoprotein Hormones, alpha Subunit/immunology
;
Humans
;
Immune Sera
;
Lysine
;
Molecular Sequence Data
;
Peptide Fragments/immunology
;
Protein Conformation
;
Rabbits/immunology
;
Sequence Homology, Nucleic Acid
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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