Publication Date:
2011-08-27
Description:
Cell-to-cell communication in plants includes the selective trafficking of transcription factors and other signals through plasmodesmata. The KNOTTED1 (KN1) homeobox (KNOX) family transcription factors, which use this pathway, are essential for stem cell establishment and/or maintenance. Here we show that KN1 trafficking requires the chaperonin complex, which belongs to a group of cytosolic chaperones that fold specific substrate proteins. Genetic and physical interaction data show a functional relevance for chaperonins in KNOX family-dependent stem cell maintenance. Furthermore, tissue-specific complementation assays indicate a mechanistic basis for chaperonin function during the posttranslocational refolding process. Our study shows that chaperonins are essential for the cell-to-cell trafficking of a subset of mobile transcription factors and demonstrates the importance of chaperonin-dependent protein trafficking for plant stem cell function.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Xu, Xianfeng Morgan -- Wang, Jing -- Xuan, Zhenyu -- Goldshmidt, Alexander -- Borrill, Philippa G M -- Hariharan, Nisha -- Kim, Jae Yean -- Jackson, David -- New York, N.Y. -- Science. 2011 Aug 26;333(6046):1141-4. doi: 10.1126/science.1205727.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21868675" target="_blank"〉PubMed〈/a〉
Keywords:
Arabidopsis/cytology/genetics/growth & development/*metabolism
;
Arabidopsis Proteins/chemistry/metabolism
;
*Cell Communication
;
Chaperonins/*metabolism
;
Cytoskeleton/physiology
;
Homeodomain Proteins/chemistry/genetics/*metabolism
;
Meristem/*cytology/physiology
;
Mutation
;
Plant Leaves/cytology/growth & development/metabolism
;
Plant Proteins/chemistry/genetics/*metabolism
;
Plasmodesmata/*metabolism
;
Protein Folding
;
Protein Transport
;
Recombinant Fusion Proteins/chemistry/metabolism
;
Zea mays/cytology/genetics/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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