Publication Date:
2013-05-25
Description:
Although intercellular bridges resulting from incomplete cytokinesis were discovered in somatic Drosophila tissues decades ago, the impact of these structures on intercellular communication and tissue biology is largely unknown. In this work, we demonstrate that the ~250-nanometer-diameter somatic ring canals permit diffusion of cytoplasmic contents between connected cells and across mitotic clone boundaries and enable the equilibration of protein between transcriptionally mosaic follicle cells in the Drosophila ovary. We obtained similar, although more restricted, results in the larval imaginal discs. Our work illustrates the lack of cytoplasmic autonomy in these tissues and suggests a role for somatic ring canals in promoting homogeneous protein expression within the tissue.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819220/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819220/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉McLean, Peter F -- Cooley, Lynn -- GM043301/GM/NIGMS NIH HHS/ -- GM091791/GM/NIGMS NIH HHS/ -- P41 GM103313/GM/NIGMS NIH HHS/ -- R01 GM043301/GM/NIGMS NIH HHS/ -- RC1 GM091791/GM/NIGMS NIH HHS/ -- T32 GM007499/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2013 Jun 21;340(6139):1445-7. doi: 10.1126/science.1234887. Epub 2013 May 23.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Genetics, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/23704373" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Cycle
;
Cytoplasm/*metabolism
;
Cytoplasmic Structures/*metabolism/*ultrastructure
;
Diffusion
;
Drosophila
;
Drosophila Proteins/*metabolism
;
Female
;
Giant Cells/ultrastructure
;
Green Fluorescent Proteins/*metabolism
;
Imaginal Discs/*metabolism/*ultrastructure
;
Microscopy, Electron
;
Mitosis
;
Ovarian Follicle/cytology/metabolism/ultrastructure
;
*Protein Transport
;
Recombination, Genetic
;
Transcription, Genetic
;
Transgenes
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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