Publication Date:
1996-06-28
Description:
Human immunodeficiency virus-type 1 (HIV-1) entry requires fusion cofactors on the CD4+ target cell. Fusin, a heterotrimeric GTP-binding protein (G protein)-coupled receptor, serves as a cofactor for T cell line-tropic isolates. The chemokines RANTES, MIP-1alpha, and MIP-1beta, which suppress infection by macrophage-tropic isolates, selectively inhibited cell fusion mediated by the corresponding envelope glycoproteins (Envs). Recombinant CC CKR5, a G protein-coupled receptor for these chemokines, rendered CD4-expressing nonhuman cells fusion-competent preferentially with macrophage-tropic Envs. CC CKR5 messenger RNA was detected selectively in cell types susceptible to macrophage-tropic isolates. CC CKR5 is thus a fusion cofactor for macrophage-tropic HIV-1 strains.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Alkhatib, G -- Combadiere, C -- Broder, C C -- Feng, Y -- Kennedy, P E -- Murphy, P M -- Berger, E A -- New York, N.Y. -- Science. 1996 Jun 28;272(5270):1955-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, Maryland 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8658171" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Animals
;
Antigens, CD4/physiology
;
Cell Fusion
;
Chemokine CCL3
;
Chemokine CCL4
;
Chemokine CCL5/metabolism/pharmacology
;
Chemokines/*metabolism/pharmacology
;
Gene Products, env/physiology
;
Giant Cells/metabolism
;
HIV-1/pathogenicity/*physiology
;
HeLa Cells
;
Humans
;
Macrophage Inflammatory Proteins
;
Macrophages/*virology
;
Membrane Fusion
;
Mice
;
Monokines/metabolism/pharmacology
;
Receptors, CCR5
;
Receptors, Cytokine/*physiology
;
Receptors, HIV/*physiology
;
Recombinant Proteins/pharmacology
;
T-Lymphocytes/virology
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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