Publication Date:
2011-12-14
Description:
Cytoplasmic dynein is a homodimeric AAA+ motor that transports a multitude of cargos toward the microtubule minus end. How the two catalytic head domains interact and move relative to each other during processive movement is unclear. Here, we tracked the relative positions of both heads with nanometer precision and directly observed the heads moving independently along the microtubule. The heads remained widely separated, and their stepping behavior varied as a function of interhead separation. One active head was sufficient for processive movement, and an active head could drag an inactive partner head forward. Thus, dynein moves processively without interhead coordination, a mechanism fundamentally distinct from the hand-over-hand stepping of kinesin and myosin.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033606/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4033606/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉DeWitt, Mark A -- Chang, Amy Y -- Combs, Peter A -- Yildiz, Ahmet -- GM08295/GM/NIGMS NIH HHS/ -- GM094522/GM/NIGMS NIH HHS/ -- R01 GM094522/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2012 Jan 13;335(6065):221-5. doi: 10.1126/science.1215804. Epub 2011 Dec 8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physics, University of California, Berkeley, CA 94720, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22157083" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphate/metabolism
;
Cytoplasm/*metabolism
;
Dyneins/*chemistry/*metabolism
;
Microtubules/*metabolism
;
Models, Biological
;
Models, Molecular
;
Protein Multimerization
;
Protein Structure, Tertiary
;
Recombinant Fusion Proteins/chemistry/metabolism
;
Saccharomyces cerevisiae Proteins/*chemistry/*metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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