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  • 1
    Electronic Resource
    Electronic Resource
    Abnormal Gene Expressions of Stroma Cells in Patients with Tuberous Sclerosis (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 615 (1991), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1991.tb37764.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genes, enzymes and membrane proteins of the nitrate respiration system ofEscherichia coli (1972)
    Springer
    The journal of membrane biology 9 (1972), S. 195-207 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A method was devised to isolate mutants carrying deletions through several genetic loci (chlD + andchlA +) which are involved in the membrane-bound nitrate respiratory complex ofEscherichia coli. Specific λ transducing phages were used to reintroduce these genes. Comparisons of membrane fractions from these transduced strains showed five membrane proteins that are necessary for the formation of an active nitrate respiration system. Two particular bacterial genes (chlD + andchlA +) were shown to control these five membrane proteins.Three of the proteins specified bychlA +, appear to be constitutively controlled and always present in the membrane ofE. coli irrespective of growth conditions, while the other two proteins, specified bychlD +, appear to be induced byanaerobic growth in the presence of nitrate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868052
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  • 3
    Electronic Resource
    Electronic Resource
    A colicin-tolerant mutant of Escherichia coli with reduced levels of cyclic AMP and a strong bias towards λ lysogeny (1973)
    Springer
    Molecular genetics and genomics 120 (1973), S. 1-16 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A colicin-tolerant mutant of Escherichia coli which is temperature-dependent for tolerance and unable to grow at 40° without the addition of salt, plates λ+ wild-type phage with a reduced frequency and very turbid plaques at 30°, failing to form any plaques at 40° even though the cells become phage infected. Phage mutants λcI, λcII, λcY42 and λvir form clear plaques and plate with equal efficiency on the mutant and its parent strain at both temperatures. The results show that over 95% of the phage infected cells become lysogenized after λ+ phage infection. In contrast, the temperate phage P1 formed normal turbid plaques with equal frequency on both bacterial strains, but phage ϕ80 forms normal turbid plaques at 30° and clear (non-lysogenic) plaques at 40°. An investigation of the mutant strain showed that it not only lacked a large molecular weight component from its cell envelope but also had reduced capacity to synthesize cyclic-3′,5′-AMP. It is proposed that the mutation(s) in the colicin-tolerant mutant gives rise to a number of alterations, one of which changes the cell envelope and leads to the reduction of intracellular cyclic AMP and the other giving rise to an altered RNA polymerase specificity. These alterations lead to a changed ratio of the products of the λ-genes involved in the control of lysogeny and give rise to a bias towards the lysogenic response. The cIII gene product of λ is not as essential to lysogeny as cII or cI and therefore possibly acts as an internal control of other λ functions (e.g. cro).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332980
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  • 4
    Electronic Resource
    Electronic Resource
    The E. coli cell surface: Isolation of λ transducing phages carrying the tolPAB cluster (1973)
    Springer
    Molecular genetics and genomics 121 (1973), S. 325-335 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The isolation of λ transducing phages carrying the tolPAB cluster is described. These genes map between gltA and gal in Escherichia coli, and thus are relatively close to attλ. To isolate these transducing phages, it was necessary to use a strain deleted of most of the intervening genes (nadA to chlD) between tolPAB and attλ. Using a lysogen of such a deletion strain, several defective λdtol phages were isolated that carry different amounts of the tolPAB cluster. All of these λdtolPAB phages were defective in both lysogenization and vegetative growth, and in this respect were similar to λdgal transducing phages. The usefulness of such specialized transducing phages in studying the cell surface is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00433231
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  • 5
    Electronic Resource
    Electronic Resource
    Chemically defined medium for the production of biologically active substances of CHO cells (1992)
    Springer
    Cytotechnology 10 (1992), S. 9-14 
    Details
    ISSN: 1573-0778
    Keywords: recombinant CHO ; hGH ; dhfr ; MTX ; chemically defined medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene. The cells grew well in the alpha MEM medium supplemented with 5% dialyzed fetal calf serum (dFCS), but not with less than 1% dFCS. Therefore we examined various medium components and obtained an improved medium which supported cell growth at low serum concentrations. The production of hGH by the cells was also enhanced in this medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376095
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  • 6
    Electronic Resource
    Electronic Resource
    A new culture system for the cultivation of mammalian cells for the production of several biologically active substances (1993)
    Springer
    Cytotechnology 13 (1993), S. 143-148 
    Details
    ISSN: 1573-0778
    Keywords: cell culture ; cell culture apparatus ; dialysis membrane ; perfusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We recently developed a new dialysis culture system (termed LIFROC-device) for the cultivation of lymphokine-activated killer cells (Murataet al., 1990, 1991). In the present study, we applied the LIFROC-device (400 ml culture vessel) to the cultivation of mammalian cells for the production of biologically active substances. We cultured mouse-mouse hybridoma TP-709, secreting anti-tissue plasminogen activator (tPA) monoclonal antibody (mAb), recombinant CHO GT19, secreting hGH, and human melanoma Bowes cells, secreting tPA. With the LIFROC-device, TP-709 grew to a maximal cell density of 3.8×106 cells/ml and and produced 480 μg/ml (192 mg in total) of mAb. GT19 reached a cell density of 2.2×106 cells/ml and produced 302 μg/ml (120 mg in total) of hGH. Bowes cells expanded to 4.4×106 cells/ml and secreted 8.5 μg/ml (3.3 mg in total) of tPA. The protein concentration in the culture broths of the LIFROC-device became 7–200 times higher than that of batch culture. Thus, the LIFROC-device can be applied to protein production as well as cell growth with high efficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00749941
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  • 7
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a DNA fragment containing various kinds of repetitive sequences located on human chromosome 21 (1993)
    Springer
    Journal of human genetics 38 (1993), S. 243-255 
    Details
    ISSN: 1435-232X
    Keywords: human chromosome 21 ; repetitive sequence ; retroposon ; mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In order to investigate the repetitive sequences located on human chromosome 21, we have isolated DNA fragments containing Alu sequences. One of the clones, p1, was chosen for further study, because it contained repetitive sequences different from the Alu sequence. Nucleotide sequence analysis of p1 indicates that p1 contains L1 and O-family sequences. Interestingly, when the L1 sequence was used as a probe, a discrete band of 5 kb was seen in HindIII-digested DNA from somatic cell hydbrids containing human chromosome 21 as the sole human chromosome. The L1 sequence was rearranged and was interrupted by O-family sequence, which wasd flanked by 6 bp target site dup litations. Since all three repetitive sequences are known to act as retroposons, these results imply that there is an integration hot spot on human chromosome 21. The sequence was mapped within 21q11–21.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01874135
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and characterization of tunicamycin resistant mutants from Chinese Hamster ovary cellsAbbreviations used: TM, tunicamycin; TMR, tunicamycin-resistant; CHO, Chinese hamster ovary; EMS, ethylmethanesulfonate; VSV, Vesicular stomatitis virus; PHA, phytohemagglutinin from phaseolus vulgaris; ConA, concanavalin A; NTE, 0.13 M NaCl, 50 mM Tris-hydrochloride and 0.01 M ethylenediamine tetraacetic acid. (1979)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 101 (1979), S. 149-156 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Stable clones selected for resistance to tunicamycin (TM) have been isolated from Chinese Hamster Ovary (CHO) cells. The TMR phenotype is stable for more than nine months in the absence of the drug. The morphology of TMR mutant varies from epitheloid to abnormally elongate. The mutants do not display cross-resistance for ConA but are slightly cross-resistant to PHA. Biochemically labeled membrane proteins and glycoprotein of Vesicular stomatitis virus (VSV) grown in the TMR mutants revealed that the incorporation of radioactive glucosamine was markedly reduced in the mutants. The results indicate that TMR cells are a novel type of membrane mutant.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041010117
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  • 9
    Electronic Resource
    Electronic Resource
    Alteration in cyclic adenosine 3′:5′-monophosphate binding proteins during the phenotypic change of mouse macrophages transformed by a temperature-sensitive mutant (tsA640) of SV40 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 127 (1986), S. 162-166 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: By using a photoaffinity ligand, cell extracts from transformed macrophages that were established by infection with temperature-sensitive mutants (tsA640) of simian virus 40 (SV40) were examined for cyclic adenosine 3′:5′-monophosphate (cAMP)-binding proteins. At the nonpermissive temperature for SV40 large T antigen, 39.0°C, no significant cAMP-binding proteins could be detected, such as primary mouse macrophages. At the permissive temperature of 33.0°C, cAMP-binding proteins appeared later than SV40 T antigen expression and cellular DNA synthesis. The profile of cAMP-binding proteins was similar to that of resting, but not proliferating, mouse clonal fibroblasts (BALB/c 3T3). These and previous results suggest that SV40 T antigen influences the expression of cAMP-binding proteins in tsA640-transformed macrophages; the large/small T antigen converts the profile of cAMP-binding proteins from macrophage to fibroblastic cells.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041270119
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  • 10
    Electronic Resource
    Electronic Resource
    Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 136 (1988), S. 421-430 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We compared proliferation and survival of various syngeneic transformed cell lines under conditions of depletion of 15 amino acids in Dulbecco-Eagle's medium. We used a normal fibroblast line 3Y1 and 22 transformed sublines of 3Y1 which had been induced by one of seven transforming agents-simian virus 40, mouse polyomavirus, adenovirus type 12, E1A gene of adenovirus type 12, cDNA of Harvey murine sarcoma virus, Rous sarcoma virus, or N-methyl-N′-nitro-N-nitrosoguanidine. Unlike other untransformed cells examined (mouse BALB/c-3T3 line, mouse NlH-3T3 line, and primary Fischer rat embryo fibroblasts), 3Y1 ceased to proliferate and accumulated in a viable state with a G1-phase DNA content under 14 singular deprivations of amino acid. None of the transformed 3Y1 lines completely arrested in the G1 phase of the cell cycle and each showed different levels of survival, depending on each transforming agent. As for transformed 3Y1 cells induced by a given virus or a given transforming gene, any one of the three sublines shared the same trend with respect to proliferation and survival. Transformed derivatives induced by N-methyl-N′-nitro-N-nitrosoguanidine showed almost the same trend in proliferation, but the patterns of survival were not uniform. Our observations suggest that the unique responses of 3Y1 to amino acid depletion are differently modified by different transforming agents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041360305
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