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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 52 (1996), S. 3-14 
    ISSN: 0006-3592
    Keywords: skin substitute ; transplantation ; keratinocyte ; barrier function ; stratum corneum ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Skin tissue may be engineered in a variety of ways. Our cultured skin substitute (Graftskin, living skin equivalent or G-LSE), Apligraf™, is an organotypic culture of skin, containing both a “dermis” and “epidermis.” The epidermis is an important functional component of skin, responsible for biologic wound closure. The epidermis possesses a stratum corneum which develops with time in culture. The stratum corneum provides barrier function properties and gives the LSE improved strength and handling characteristics. Clinical experience indicated that the stratum corneum might play an important role in improving the clinical utility of the LSE. Handling and physical characteristics improved with time in culture. We examined the LSE at different stages of epidermal maturation for barrier function and ability to persist as a graft. LSE grafted onto athymic mice before significant development of barrier function did not withstand bandage removal at 7 days postgraft. LSE grafted after barrier function had been established in vitro were able to withstand bandage removal at day 7. Corneum lipid composition and structure are critical components for barrier function. Media modifications were used in an attempt to improve the fatty acid composition of the stratum corneum. The barrier developed more rapidly and was improved in a serum-free, lipid-supplemented condition. Lipid lamellar structure was improved with 10% of the stratum corneum exhibiting broad-narrow-broad lipid lamellar arrangements similar to human skin. Fatty acid metabolism was not appreciably altered. Barrier function in vitro was 4- to 10-fold more permeable than human skin. Epidermal differentiation does not compromise engraftment or the wound healing ability of the epidermis. The stratum corneum provides features beneficial for engraftment and clinical use. © 1996 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Publication Date: 1971-04-01
    Print ISSN: 0022-2488
    Electronic ISSN: 1089-7658
    Topics: Mathematics , Physics
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  • 3
    Publication Date: 2009-12-01
    Print ISSN: 0167-8655
    Electronic ISSN: 1872-7344
    Topics: Computer Science
    Published by Elsevier
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  • 4
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    In:  Other Sources
    Publication Date: 2011-08-24
    Description: An overview of the major concepts of Controlled Ecological Life Support System (CELSS) includes an identification of environmental factors, such as gravity levels, light levels, and growth volume, that influence the type of CELSS system that can be developed. Various plant growth systems are described together with their possible space applications. Life support functions performed by plants include food production, atmosphere regeneration, and water purification. Selected relationships between biological and physical-chemical life support techniques are considered as a part of these functions. Consumers in a CELSS may be humans, animals, or microorganisms, but nutritional, water, and atmosphere requirements of humans are emphasized in this report, as they are the primary requirement drivers for a CELSS design. The human role in waste generation is discussed as it affects plant nutrient availability. The role of waste management systems in recovering nutrients for plant growth and requirements for CELSS are defined for air, water, and food. Both physical and a biological nutrient recovery/waste disposal systems are examined. The separate subsystems of a CELSS are identified and discussed. Nutrient recovery, plant irradiation, automation, and facilities equipment and applications are reviewed with special attention to direct solar irradiation using fiber optics. These subsystems, along with other environmental control systems, such as thermal, humidity, and ventilation, are essential to plant growth in the space environment.
    Keywords: Man/System Technology and Life Support
    Type: HortScience : a publication of the American Society for Horticultural Science (ISSN 0018-5345); Volume 23; 2; 275-86
    Format: text
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  • 5
    Publication Date: 2019-06-28
    Description: Parametric data for six waste management subsystems considered for use on the Space Station are compared, i.e.: (1) dry incineration; (2) wet oxidation; (3) supercritical water oxidation; (4) vapor compression distillation; (5) thermoelectric integrated membrane evaporation system; and (6) vapor phase catalytic ammonia removal. The parameters selected for comparison are on-orbit weight and volume, resupply and return to Earth logistics, power consumption, and heat rejection. Trades studies are performed on subsystem parameters derived from the most recent literature. The Boeing Engineering Trade Study (BETS), an environmental control and life support system (ECLSS) trade study computer program developed by Boeing Aerospace Company, is used to properly size the subsystems under study. The six waste treatment subsystems modeled in this program are sized to process the wastes for a 90-day Space Station mission with an 8-person crew, and an emergency supply period of 28 days. The resulting subsystem parameters are compared not only on an individual subsystem level but also as part of an integrated ECLSS.
    Keywords: MAN/SYSTEM TECHNOLOGY AND LIFE SUPPORT
    Type: NASA-CR-177422 , NAS 1.26:177422 , BAC-37
    Format: application/pdf
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